C57BL/6NTac-Cpeb4^{tm1a(EUCOMM)Wtsi}/Cnrm

Status	Available to order
EMMA ID	EM:04002
International strain name	C57BL/6NTac-Cpeb4^{tm1a(EUCOMM)Wtsi}/Cnrm
Alternative name	EPD0060_4_E10
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Cpeb4^{tm1a(EUCOMM)Wtsi}
Gene/Transgene symbol	Cpeb4
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	CNR, Consiglio Nazionale delle Ricerche
Provider affiliation	EMMA-Monterotondo Campus International Development, CNR, Consiglio Nazionale delle Ricerche
Genetic information	This mouse line originates from EUCOMM ES clone EPD0060_4_E10. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	Immune translational control by CPEB4 regulates intestinal inflammation resolution and colorectal cancer development.;Sibilio Annarita, Suñer Clara, Fernández-Alfara Marcos, Martín Judit, Berenguer Antonio, Calon Alexandre, Chanes Veronica, Millanes-Romero Alba, Fernández-Miranda Gonzalo, Batlle Eduard, Fernández Mercedes, Méndez Raúl, ;2022;iScience;25;103790; 35243213

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

IMPC phenotypes (allele matching)

increased circulating alkaline phosphatase level / IMPC
preweaning lethality, complete penetrance / IMPC

IMPC phenotypes (gene matching)

preweaning lethality, complete penetrance / IMPC
abnormal seminal vesicle morphology / IMPC
decreased circulating calcium level / IMPC
increased circulating alkaline phosphatase level / IMPC

MGI phenotypes (allele matching)

abnormal erythropoiesis / MGI
abnormal neuromuscular synapse morphology / MGI
decreased body weight / MGI
decreased body size / MGI
impaired coordination / MGI
abnormal suckling behavior / MGI
respiratory distress / MGI
nervous system phenotype / MGI
abnormal axon extension / MGI
muscle phenotype / MGI
abnormal cell physiology / MGI
abnormal neurite morphology / MGI
decreased survivor rate / MGI
absent gastric milk in neonates / MGI
postnatal lethality, incomplete penetrance / MGI
neonatal lethality, incomplete penetrance / MGI
lethality during fetal growth through weaning, incomplete penetrance / MGI
abnormal fetal derived definitive erythrocyte morphology / MGI
decreased fetal derived definitive erythrocyte cell number / MGI
abnormal phrenic nerve innervation pattern to diaphragm / MGI

MGI phenotypes (gene matching)

abnormal erythropoiesis / MGI
abnormal neuromuscular synapse morphology / MGI
decreased body weight / MGI
decreased body size / MGI
impaired coordination / MGI
abnormal suckling behavior / MGI
respiratory distress / MGI
no abnormal phenotype detected / MGI
nervous system phenotype / MGI
abnormal axon extension / MGI
muscle phenotype / MGI
behavior/neurological phenotype / MGI
abnormal cell physiology / MGI
abnormal neurite morphology / MGI
decreased survivor rate / MGI
absent gastric milk in neonates / MGI
abnormal dendritic spine morphology / MGI
postnatal lethality, incomplete penetrance / MGI
neonatal lethality, incomplete penetrance / MGI
lethality during fetal growth through weaning, incomplete penetrance / MGI
abnormal fetal derived definitive erythrocyte morphology / MGI
decreased fetal derived definitive erythrocyte cell number / MGI
abnormal phrenic nerve innervation pattern to diaphragm / MGI

Literature references

Immune translational control by CPEB4 regulates intestinal inflammation resolution and colorectal cancer development.;Sibilio Annarita, Suñer Clara, Fernández-Alfara Marcos, Martín Judit, Berenguer Antonio, Calon Alexandre, Chanes Veronica, Millanes-Romero Alba, Fernández-Miranda Gonzalo, Batlle Eduard, Fernández Mercedes, Méndez Raúl, ;2022;iScience;25;103790; 35243213
Impaired neurodevelopment by the low complexity domain of CPEB4 reveals a convergent pathway with neurodegeneration.;Shin Jihae, Salameh Johnny S, Richter Joel D, ;2016;Scientific reports;6;29395; 27381259
Antitumor T-cell function requires CPEB4-mediated adaptation to chronic endoplasmic reticulum stress.;Fernández-Alfara Marcos, Sibilio Annarita, Martin Judit, Tusquets Uxó Elsa, Malumbres Marina, Alcalde Victor, Chanes Verónica, Cañellas-Socias Adrià, Palomo-Ponce Sergio, Batlle Eduard, Méndez Raúl, ;2023;The EMBO journal;42;e111494; 36919984

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C57BL/6NTac-Cpeb4tm1a(EUCOMM)Wtsi/Cnrm