C57BL/6NTac-Asxl1tm1e(EUCOMM)Wtsi/Cnrm

Status

Available to order

EMMA IDEM:03996
International strain nameC57BL/6NTac-Asxl1tm1e(EUCOMM)Wtsi/Cnrm
Alternative nameEPD0080_1_B11
Strain typeTargeted Mutant Strains : Targeted Non-conditional
Allele/Transgene symbolAsxl1tm1e(EUCOMM)Wtsi
Gene/Transgene symbolAsxl1
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider CNR, Consiglio Nazionale delle Ricerche
Provider affiliationEMMA-Monterotondo Campus International Development, CNR, Consiglio Nazionale delle Ricerche
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0080_1_B11. For further details on the construction of this clone see the page at the IMPC portal. The targeted allele has lost the 3' loxP site. These mutations cannot be converted into conditional alleles. Click here for more information on EUCOMM final vectors.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy
Animals used for archivingheterozygous C57BL/6NTac, wild-type C57BL/6NTac
Stage of embryos2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • process of degenerative change / IMPC
  • decreased circulating glucose level / IMPC
  • hyperactivity / IMPC
  • decreased hemoglobin content / IMPC
  • impaired pupillary reflex / IMPC
  • decreased sacral vertebrae number / IMPC
  • decreased mean corpuscular hemoglobin / IMPC
  • increased lumbar vertebrae number / IMPC
  • decreased circulating iron level / IMPC
  • increased blood uric acid level / IMPC
  • vertebral fusion / IMPC
  • corneal opacity / IMPC
  • cataract / IMPC
  • decreased hematocrit / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • decreased mean corpuscular volume / IMPC
  • abnormal cornea morphology / IMPC
MGI phenotypes (gene matching)
  • cleft palate / MGI
  • increased leukocyte cell number / MGI
  • increased monocyte cell number / MGI
  • decreased leukocyte cell number / MGI
  • decreased neutrophil cell number / MGI
  • extramedullary hematopoiesis / MGI
  • increased bone marrow cell number / MGI
  • decreased bone marrow cell number / MGI
  • increased cell proliferation / MGI
  • abnormal craniofacial morphology / MGI
  • mandible hypoplasia / MGI
  • abnormal liver morphology / MGI
  • enlarged liver / MGI
  • abnormal spleen morphology / MGI
  • enlarged spleen / MGI
  • small spleen / MGI
  • spleen hyperplasia / MGI
  • abnormal thymus morphology / MGI
  • abnormal immune system cell morphology / MGI
  • decreased body weight / MGI
  • decreased body size / MGI
  • anophthalmia / MGI
  • microphthalmia / MGI
  • abnormal cornea morphology / MGI
  • corneal opacity / MGI
  • anemia / MGI
  • abnormal myelopoiesis / MGI
  • impaired hematopoiesis / MGI
  • increased malignant tumor incidence / MGI
  • increased leukemia incidence / MGI
  • increased sarcoma incidence / MGI
  • premature death / MGI
  • abnormal spleen white pulp morphology / MGI
  • abnormal bone marrow cell morphology/development / MGI
  • abnormal lymphopoiesis / MGI
  • abnormal megakaryocyte morphology / MGI
  • abnormal blood cell morphology/development / MGI
  • abnormal neutrophil physiology / MGI
  • opacity of vitreous body / MGI
  • abnormal immune system organ morphology / MGI
  • decreased hemoglobin content / MGI
  • vertebral transformation / MGI
  • abnormal hyoid bone morphology / MGI
  • thrombocytopenia / MGI
  • spleen atrophy / MGI
  • vertebral fusion / MGI
  • decreased lumbar vertebrae number / MGI
  • increased hematopoietic stem cell number / MGI
  • decreased hematopoietic stem cell number / MGI
  • decreased spleen weight / MGI
  • decreased lymphocyte cell number / MGI
  • decreased B cell number / MGI
  • decreased T cell number / MGI
  • abnormal neutrophil morphology / MGI
  • increased double-negative T cell number / MGI
  • polychromatophilia / MGI
  • abnormal skeleton morphology / MGI
  • decreased circulating glucose level / MGI
  • decreased mean corpuscular hemoglobin / MGI
  • increased apoptosis / MGI
  • abnormal common myeloid progenitor cell morphology / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • increased single-positive T cell number / MGI
  • decreased pre-B cell number / MGI
  • abnormal leukocyte morphology / MGI
  • decreased spleen red pulp amount / MGI
  • increased blood uric acid level / MGI
  • decreased erythroid progenitor cell number / MGI
  • eye opacity / MGI
  • increased sacral vertebrae number / MGI
  • increased number of Howell-Jolly bodies / MGI
  • myeloid hyperplasia / MGI
  • abnormal hematopoietic stem cell physiology / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • neonatal lethality, incomplete penetrance / MGI
  • lethality throughout fetal growth and development, complete penetrance / MGI
  • lethality throughout fetal growth and development, incomplete penetrance / MGI
  • lethality, complete penetrance / MGI

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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