IMPC phenotypes (gene matching)
B6.129P2-Gjc1tm1Kwi/Cnrm
| Status | Available to order |
| EMMA ID | EM:00331 |
| Citation information | RRID:IMSR_EM:00331 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Gjc1tm1Kwi/Cnrm |
| Alternative name | Cx45 KO (LacZ) |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Gjc1tm1Kwi |
| Gene/Transgene symbol | Gjc1 |
Information from provider
| Provider | Klaus WILLECKE |
| Provider affiliation | Molekulargenetik, Institut fuer Genetik, Universitaet Bonn |
| Genetic information | Exon 3, including the whole open reading frame of Cx45 (Gjc1), is homologously replaced by the lacZ open reading frame. The selection marker gene (neo) is still in the genome. |
| Phenotypic information | Embryonic lethal at 10.5 dpc, caused by endothelium malformation. |
| Breeding history | After blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown coat coloured offspring. This brown offspring was backcrossed to C57BL/6NCrl more than three times. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Backcrossed to C57BL/6J |
Disease and phenotype information
MGI phenotypes (gene matching)
- abnormal heart development / MGI
- abnormal heart looping / MGI
- enlarged heart / MGI
- absent trabeculae carneae / MGI
- absent atrioventricular cushions / MGI
- impaired hematopoiesis / MGI
- decreased embryo size / MGI
- embryonic growth arrest / MGI
- thin myocardium / MGI
- no phenotypic analysis / MGI
- decreased cardiac output / MGI
- embryonic growth retardation / MGI
- small placenta / MGI
- decreased cardiac muscle contractility / MGI
- increased apoptosis / MGI
- heart block / MGI
- embryonic lethality during organogenesis, complete penetrance / MGI
- increased embryonic neuroepithelium apoptosis / MGI
Literature references
- Defective vascular development in connexin 45-deficient mice.;Krüger O, Plum A, Kim J S, Winterhager E, Maxeiner S, Hallas G, Kirchhoff S, Traub O, Lamers W H, Willecke K, ;2000;Development (Cambridge, England);127;4179-93; 10976050