B6.Cg-Tg(Ins1-itTA)#Fto/Orl
| Status | Available to order |
| EMMA ID | EM:03106 |
| Citation information | RRID:IMSR_EM:03106 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(Ins1-itTA)#Fto/Orl |
| Alternative name | Tg(BAC-InstTAfto) |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Ins1-itTA)#Fto |
| Gene/Transgene symbol | Tg(Ins1-itTA)#Fto |
Information from provider
| Provider | Francois Tronche |
| Provider affiliation | Molecular Genetics Neurophysiology and Behavior, CNRS CNRS UMR7224 |
| Additional owner | Bernadette Bréant, INSERM |
| Genetic information | Mice from the BAC-InshtTA transgenic line express the tTA synthetic transcription factor into pancreatic beta-cells. When crossed with transgenic lines carrying a cDNA under the control of a tetO-dependent promoter, it allows its conditional expression, repressed in the presence of doxycycline. Efficiency was tested using target tetO reporter and was excellent. |
| Phenotypic information | We did not observe any phenotype in animals carrying Tg(BAC-InshtTA). |
| Breeding history | Backcrossed into C57BL/6 for five generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous males, wild-type C57BL/6J females |
Literature references
- Novel transgenic mice for inducible gene overexpression in pancreatic cells define glucocorticoid receptor-mediated regulations of beta cells.;Blondeau Bertrand, Sahly Iman, Massouridès Emmanuelle, Singh-Estivalet Amrit, Valtat Bérengère, Dorchene Delphine, Jaisser Frédéric, Bréant Bernadette, Tronche Francois, ;2012;PloS one;7;e30210; 22363422