B6.Cg-Tg(Ins1-itTA)#Fto/Orl
| Status | Available to order |
| EMMA ID | EM:03106 |
| International strain name | B6.Cg-Tg(Ins1-itTA)#Fto/Orl |
| Alternative name | Tg(BAC-InstTAfto) |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Ins1-itTA)#Fto |
| Gene/Transgene symbol | Tg(Ins1-itTA)#Fto |
Information from provider
| Provider | Francois Tronche |
| Provider affiliation | Molecular Genetics Neurophysiology and Behavior, CNRS CNRS UMR7224 |
| Additional owner | Bernadette Bréant, INSERM |
| Genetic information | Mice from the BAC-InshtTA transgenic line express the tTA synthetic transcription factor into pancreatic beta-cells. When crossed with transgenic lines carrying a cDNA under the control of a tetO-dependent promoter, it allows its conditional expression, repressed in the presence of doxycycline. Efficiency was tested using target tetO reporter and was excellent. |
| Phenotypic information | We did not observe any phenotype in animals carrying Tg(BAC-InshtTA). |
| Breeding history | Backcrossed into C57BL/6 for five generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous , wild-type C57BL/6J |
Literature references
- Novel transgenic mice for inducible gene overexpression in pancreatic cells define glucocorticoid receptor-mediated regulations of beta cells.;Blondeau Bertrand, Sahly Iman, Massouridès Emmanuelle, Singh-Estivalet Amrit, Valtat Bérengère, Dorchene Delphine, Jaisser Frédéric, Bréant Bernadette, Tronche Francois, ;2012;PloS one;7;e30210; 22363422