STOCK Mgmttm1Iz Tg(CRP-cre)1Iz/Cnrm

Status

Available to order

EMMA IDEM:00262
Citation informationRRID:IMSR_EM:00262 

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International strain nameSTOCK Mgmttm1Iz Tg(CRP-cre)1Iz/Cnrm
Alternative namefloxed(mgmt)-Tg/hCRP-Cre
Strain typeTransgenic Strains
Allele/Transgene symbolTg(CRP-cre)1Iz, Mgmttm1Iz
Gene/Transgene symbolTg(CRP-cre)1Iz, Mgmt

Information from provider

ProviderManfred Rajewsky
Provider affiliationInstitut fuer Zellbiologie (Tumorforschung), Universitaetsklinikum Essen
Genetic informationThe cre recombinase gene was cloned under control of the human C-reactive protein (hCRP) promoter. The hCRP-promoter is inducible by lipopolysaccharide (LPS) or interleukin 6. Upon induction the cre gene is strongly expressed in hepatocytes, exclusively. The amount of cre-mediated deletion can be controlled by the amount of LPS injected. The percentage of deletion is in the range of 13 to 45%. Exon V of Mgmt was flanked by two loxP-sites. This exon can be spliced out by cre recombinase thereby inactivating the DNA repair activity of the MGMT protein.
Phenotypic informationNo major defects observed. The Tg(CRP-cre) transgene allows the liver-specific, inducible expression of cre recombinase.
References
  • Conditional liver-specific "knock-out" of the murine genes encoding 06-Alkylguanine-DNA Alkyltransferase (AGT) and AP-Endonuclease (APE).;Schweer T, Rajewsky MF;1998;J Biomol Struct Dyn.;16;180-1 (Abstr.);

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

IMPC phenotypes (gene matching)
  • decreased prepulse inhibition / IMPC
MGI phenotypes (gene matching)
  • increased T cell derived lymphoma incidence / MGI
  • increased susceptibility to injury / MGI
  • increased circulating glucose level / MGI
  • increased physiological sensitivity to xenobiotic / MGI
  • small pancreatic islets / MGI
  • increased sensitivity to induced morbidity/mortality / MGI

Literature references

  • Conditional liver-specific "knock-out" of the murine genes encoding 06-Alkylguanine-DNA Alkyltransferase (AGT) and AP-Endonuclease (APE).;Schweer T, Rajewsky MF;1998;J Biomol Struct Dyn.;16;180-1 (Abstr.);

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

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Practical information

Example health report
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