MGI phenotypes (allele matching)
B6JIcoCrl.129P2-Rad52tm1Aps/Cnrm
| Status | Available to order |
| EMMA ID | EM:02592 |
| Citation information | RRID:IMSR_EM:02592 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6JIcoCrl.129P2-Rad52tm1Aps/Cnrm |
| Alternative name | RAD52 mutant |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Rad52tm1Aps |
| Gene/Transgene symbol | Rad52 |
Information from provider
| Provider | Albert Pastink |
| Provider affiliation | IHB, LUMC |
| Genetic information | To inactivate the mouse Rad52 gene, a targeting construct was generated in which exon 3, that is located in the conserved part of the gene, was replaced by a neomycin resistance marker. |
| Phenotypic information | Homozygous Rad52 mutant animals do not show gross abnormalities or defects in fertility or in the immune system. At the cellular level, inactivation of both Rad52 alleles results in a reduced frequency of homologous recombination. Unlike yeast Rad52 mutant cells, ablation of Rad52 in mouse cells does not lead to an increased sensitivity to DNA damaging agents. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Literature references
- Targeted inactivation of mouse RAD52 reduces homologous recombination but not resistance to ionizing radiation.;Rijkers T, Van Den Ouweland J, Morolli B, Rolink A G, Baarends W M, Van Sloun P P, Lohman P H, Pastink A, ;1998;Molecular and cellular biology;18;6423-9; 9774658
- Inactivation of RAD52 aggravates RAD54 defects in mice but not in Schizosaccharomyces pombe.;de Vries Femke A T, Zonneveld José B M, van Duijn-Goedhart Annemarie, Roodbergen Marianne, Boei Jan, van Buul Paul P W, Essers Jeroen, van Steeg Harry, van Zeeland Albert A, van Benthem Jan, Pastink Albert, ;2005;DNA repair;4;1121-8; 16009599
- Suppression of retroviral infection by the RAD52 DNA repair protein.;Lau Alan, Kanaar Roland, Jackson Stephen P, O'Connor Mark J, ;2004;The EMBO journal;23;3421-9; 15297876