MGI phenotypes (allele matching)
B6.129S7-Aox4tm1Enga/Cnrm
| Status | Available to order |
| EMMA ID | EM:02590 |
| Citation information | RRID:IMSR_EM:02590 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129S7-Aox4tm1Enga/Cnrm |
| Alternative name | C57BL/6-AOH2_KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Aox4tm1Enga |
| Gene/Transgene symbol | Aox4 |
Information from provider
| Provider | Enrico Garattini |
| Provider affiliation | Biochimica e farmacologia molecolare, Istituto di Ricerche Farmacologiche "Mario Negri" |
| Genetic information | Targeting construct is derived from 129/Sv DNA. Exon 22 and surrounding intron sequences were replaced by the neomycin resistance gene. |
| Phenotypic information | Knock-out animals are viable and fertile, although they have an absolute deficit of retinaldehyde oxidase activity in the Harderian gland, decreased retinoic acid levels, and down-regulation of retinoid-dependent genes. Moreover they show thickening of the epidermis in basal conditions and after UV light exposure. |
| Breeding history | The knock-out line was obtained upon ES cell injection in B6D2F1 blastocyst and successively backcrossed to C57BL/6N for 9 generations. Heterozygous animals were maintained on C57BL/6N background in SPF facility. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Backcrossed to C57BL/6J |
Disease and phenotype information
Literature references
- Role of the molybdoflavoenzyme aldehyde oxidase homolog 2 in the biosynthesis of retinoic acid: generation and characterization of a knockout mouse.;Terao Mineko, Kurosaki Mami, Barzago Maria Monica, Fratelli Maddalena, Bagnati Renzo, Bastone Antonio, Giudice Chiara, Scanziani Eugenio, Mancuso Alessandra, Tiveron Cecilia, Garattini Enrico, ;2009;Molecular and cellular biology;29;357-77; 18981221