C57BL/6-Dpp4tm1Nwa/Orl

Status

Available to order

EMMA IDEM:02577
International strain nameC57BL/6-Dpp4tm1Nwa/Orl
Alternative nameC57Bl/6_DPP4-/-
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolDpp4tm1Nwa
Gene/Transgene symbolDpp4

Information from provider

ProviderDidier MARGUET
Provider affiliationCentre D
Genetic informationDeletion in the CD26/Dpp4 gene which inactivated the expression of the DPP4 enzyme. A 2.3-kb PstI-SphI fragment containing exons 1 and 2, was replaced with the non floxed PGKNeo cassette.
Phenotypic informationEnhanced insulin secretion and improved glucose tolerance in mice lacking CD26/Dpp4.
Breeding historySince its origin, the strain has been inbred on C57BL/6 strain.
References
  • Enhanced insulin secretion and improved glucose tolerance in mice lacking CD26.;Marguet D, Baggio L, Kobayashi T, Bernard A M, Pierres M, Nielsen P F, Ribel U, Watanabe T, Drucker D J, Wagtmann N, ;2000;Proceedings of the National Academy of Sciences of the United States of America;97;6874-9; 10823914
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivinghomozygous C57BL/6J
Breeding at archiving centreWe have frozen sperm under two protocols: - MTG protocol (heterozygous males) - CARD protocol (homozygous males). Please inform us if you have a preference for one of them when you submit a request.

Disease and phenotype information

IMPC phenotypes (gene matching)
  • abnormal auditory brainstem response / IMPC
  • decreased lymphocyte cell number / IMPC
  • improved glucose tolerance / IMPC
MGI phenotypes (allele matching)
  • decreased body weight / MGI
  • increased circulating insulin level / MGI
  • increased circulating glucagon level / MGI
  • increased insulin secretion / MGI
  • improved glucose tolerance / MGI
MGI phenotypes (gene matching)
  • decreased body weight / MGI
  • increased circulating insulin level / MGI
  • increased circulating glucagon level / MGI
  • increased insulin secretion / MGI
  • improved glucose tolerance / MGI

Literature references

  • Enhanced insulin secretion and improved glucose tolerance in mice lacking CD26.;Marguet D, Baggio L, Kobayashi T, Bernard A M, Pierres M, Nielsen P F, Ribel U, Watanabe T, Drucker D J, Wagtmann N, ;2000;Proceedings of the National Academy of Sciences of the United States of America;97;6874-9; 10823914
  • Prediabetes blunts DPP4 genetic control of postprandial glycaemia and insulin secretion.;Patarrão Rita S, Duarte Nádia, Coelho Inês, Ward Joey, Ribeiro Rogério T, Meneses Maria João, Andrade Rita, Costa João, Correia Isabel, Boavida José Manuel, Duarte Rui, Gardete-Correia Luís, Medina José Luís, Pell Jill, Petrie John, Raposo João F, Macedo Maria Paula, Penha-Gonçalves Carlos, ;2022;Diabetologia;65;861-871; 35190847
  • Protocols for staining of bile canalicular and sinusoidal networks of human, mouse and pig livers, three-dimensional reconstruction and quantification of tissue microarchitecture by image processing and analysis.;Hammad Seddik, Hoehme Stefan, Friebel Adrian, von Recklinghausen Iris, Othman Amnah, Begher-Tibbe Brigitte, Reif Raymond, Godoy Patricio, Johann Tim, Vartak Amruta, Golka Klaus, Bucur Petru O, Vibert Eric, Marchan Rosemarie, Christ Bruno, Dooley Steven, Meyer Christoph, Ilkavets Iryna, Dahmen Uta, Dirsch Olaf, Böttger Jan, Gebhardt Rolf, Drasdo Dirk, Hengstler Jan G, ;2014;Archives of toxicology;88;1161-83; 24748404
  • Dipeptidyl Peptidase-4 Is a Pro-Recovery Mediator During Acute Hepatotoxic Damage and Mirrors Severe Shifts in Kupffer Cells.;Duarte Nádia, Coelho Inês, Holovanchuk Denys, Inês Almeida Joana, Penha-Gonçalves Carlos, Paula Macedo Maria, ;2018;Hepatology communications;2;1080-1094; 30202822

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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