129-Ncf1^tm1Hbd/H

Status	Available to order
EMMA ID	EM:00255
International strain name	129-Ncf1^tm1Hbd/H
Alternative name	p47 phox
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Ncf1^tm1Hbd
Gene/Transgene symbol	Ncf1

Information from provider

Provider	Jurgen Roes
Provider affiliation	Windeyer Institute of Medical Sciences, University College London
Genetic information	Disruption of the Ncf1 gene by insertion of neo gene in exon 3.
Phenotypic information	Moderate breeder, impaired innate immunity.
References	Ym1 is a neutrophil granule protein that crystallizes in p47phox-deficient mice.;Harbord Marcus, Novelli Marco, Canas Benito, Power David, Davis Christine, Godovac-Zimmermann Jasminka, Roes Jurgen, Segal Anthony W, ;2002;The Journal of biological chemistry;277;5468-75; 11733538

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI allele-associated human disease models

chronic granulomatous disease / DOID:3265

Orphanet associated rare diseases, based on orthologous gene matching

Chronic granulomatous disease / Orphanet_379

IMPC phenotypes (gene matching)

abnormal heart morphology / IMPC
abnormal behavior / IMPC
decreased thigmotaxis / IMPC
enlarged spleen / IMPC
abnormal spleen morphology / IMPC
abnormal lymph node morphology / IMPC
enlarged lymph nodes / IMPC
abnormal eye morphology / IMPC
hyperplasia / IMPC
decreased anxiety-related response / IMPC
small kidney / IMPC
abnormal kidney morphology / IMPC
increased heart atrium size / IMPC
abnormal skin morphology / IMPC
abnormal lung morphology / IMPC
single kidney / IMPC
enlarged kidney / IMPC
decreased locomotor activity / IMPC

MGI phenotypes (allele matching)

immune system phenotype / MGI
abnormal lung morphology / MGI
skin lesions / MGI
abnormal neutrophil physiology / MGI
granulomatous inflammation / MGI
abnormal bile duct morphology / MGI
liver abscess / MGI
abnormal vascular endothelial cell physiology / MGI

MGI phenotypes (gene matching)

abnormal angiogenesis / MGI
abnormal heart morphology / MGI
altered response to myocardial infarction / MGI
abnormal lung morphology / MGI
skin lesions / MGI
abnormal spatial learning / MGI
reduced long term potentiation / MGI
abnormal cardiovascular system physiology / MGI
cardiac hypertrophy / MGI
abnormal postnatal growth / MGI
abnormal inflammatory response / MGI
abnormal respiratory system physiology / MGI
abnormal tracheal smooth muscle morphology / MGI
increased susceptibility to infection / MGI
decreased susceptibility to viral infection / MGI
abnormal macrophage physiology / MGI
abnormal neutrophil physiology / MGI
granulomatous inflammation / MGI
dilated heart left ventricle / MGI
increased systemic arterial blood pressure / MGI
abnormal bile duct morphology / MGI
abnormal cardiac muscle contractility / MGI
enhanced long term potentiation / MGI
decreased vasoconstriction / MGI
increased angiotensin I-converting enzyme activity / MGI
abnormal locomotor activation / MGI
liver abscess / MGI
increased circulating renin level / MGI
abnormal intestinal goblet cell morphology / MGI
oxidative stress / MGI
abnormal vascular endothelial cell physiology / MGI
abnormal vascular wound healing / MGI
abnormal response to infection / MGI
impaired granulocyte bactericidal activity / MGI
increased acute inflammation / MGI
immune system phenotype / MGI
abnormal vascular smooth muscle physiology / MGI
impaired cued conditioning behavior / MGI
increased sensitivity to induced morbidity/mortality / MGI
increased lung compliance / MGI
decreased airway resistance / MGI

Literature references

Ym1 is a neutrophil granule protein that crystallizes in p47phox-deficient mice.;Harbord Marcus, Novelli Marco, Canas Benito, Power David, Davis Christine, Godovac-Zimmermann Jasminka, Roes Jurgen, Segal Anthony W, ;2002;The Journal of biological chemistry;277;5468-75; 11733538
Inflammatory monocytes and neutrophils are licensed to kill during memory responses in vivo.;Narni-Mancinelli Emilie, Soudja Saidi M'Homa, Crozat Karine, Dalod Marc, Gounon Pierre, Geissmann Frédéric, Lauvau Grégoire, ;2011;PLoS pathogens;7;e1002457; 22241983
Molecular insights of p47phox phosphorylation dynamics in the regulation of NADPH oxidase activation and superoxide production.;Meijles Daniel N, Fan Lampson M, Howlin Brendan J, Li Jian-Mei, ;2014;The Journal of biological chemistry;289;22759-22770; 24970888
p47^phox-Dependent Oxidant Signalling through ASK1, MKK3/6 and MAPKs in Angiotensin II-Induced Cardiac Hypertrophy and Apoptosis.;Liu Fangfei, Fan Lampson M, Geng Li, Li Jian-Mei, ;2021;Antioxidants (Basel, Switzerland);10;102269; 34572995
Inhibition of endothelial Nox2 activation by LMH001 protects mice from angiotensin II-induced vascular oxidative stress, hypertension and aortic aneurysm.;Fan Lampson M, Liu Fangfei, Du Junjie, Geng Li, Li Jian-Mei, ;2022;Redox biology;51;; 35276443

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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129-Ncf1tm1Hbd/H