MGI phenotypes (allele matching)
B6.129P2-Gjd2tm4Kwi/Cnrm
| Status | Available to order |
| EMMA ID | EM:02510 |
| Citation information | RRID:IMSR_EM:02510 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Gjd2tm4Kwi/Cnrm |
| Alternative name | Cx36flox(CFP) |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Gjd2tm4Kwi |
| Gene/Transgene symbol | Gjd2 |
Information from provider
| Provider | Klaus WILLECKE |
| Provider affiliation | Molekulargenetik, Institut fuer Genetik, Universitaet Bonn |
| Genetic information | Instead of exon 1 and 2 of Cx36 (Gjd2) a loxP sites-flanked construct is inserted containing both exons and the intron of Cx36 and a frt sites-flanked neomycin selection cassette. After cre recombinase activity a cyan fluorescent protein reporter gene is expressed under the control of the endogenous Cx36 promoter. |
| Phenotypic information | No major defects were observed in the floxed state. After cre rembinase-mediated deletion homozygous Cx36 knock-out mice show visual transmission defects and reduced gamma oscillation. |
| Breeding history | After blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown coat coloured offspring. This brown offspring was backcrossed to C57BL/6NCrl more than three times. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Backcrossed to C57BL/6J |
Disease and phenotype information
MGI phenotypes (gene matching)
- abnormal cued conditioning behavior / MGI
- abnormal CNS synaptic transmission / MGI
- abnormal nervous system electrophysiology / MGI
- abnormal inhibitory postsynaptic potential / MGI
- abnormal excitatory postsynaptic potential / MGI
- no phenotypic analysis / MGI
- decreased insulin secretion / MGI
- abnormal single cell response / MGI
- abnormal nerve fiber response / MGI
- abnormal pancreatic beta cell physiology / MGI
- abnormal insulin secretion / MGI
- nervous system phenotype / MGI
- abnormal brain wave pattern / MGI
- abnormal retinal ganglion layer morphology / MGI
- endocrine/exocrine gland phenotype / MGI
- digestive/alimentary phenotype / MGI
- behavior/neurological phenotype / MGI
- vision/eye phenotype / MGI
- abnormal action potential / MGI
Literature references
- A new conditional mouse mutant reveals specific expression and functions of connexin36 in neurons and pancreatic beta-cells.;Wellershaus Kerstin, Degen Joachim, Deuchars Jim, Theis Martin, Charollais Anne, Caille Dorothée, Gauthier Benoit, Janssen-Bienhold Ulrike, Sonntag Stephan, Herrera Pedro, Meda Paolo, Willecke Klaus, ;2008;Experimental cell research;314;997-1012; 18258229
- Amacrine Cells Forming Gap Junctions With Intrinsically Photosensitive Retinal Ganglion Cells: ipRGC Types, Neuromodulator Contents, and Connexin Isoform.;Harrison Krystal R, Chervenak Andrew P, Resnick Sarah M, Reifler Aaron N, Wong Kwoon Y, ;2021;Investigative ophthalmology & visual science;62;10; 33410914