B6.129P2-Gjd2tm4Kwi/Cnrm
Status | Available to order |
EMMA ID | EM:02510 |
International strain name | B6.129P2-Gjd2tm4Kwi/Cnrm |
Alternative name | Cx36flox(CFP) |
Strain type | Targeted Mutant Strains : Conditional mutation |
Allele/Transgene symbol | Gjd2tm4Kwi |
Gene/Transgene symbol | Gjd2 |
Information from provider
Provider | Klaus WILLECKE |
Provider affiliation | Molekulargenetik, Institut fuer Genetik, Universitaet Bonn |
Genetic information | Instead of exon 1 and 2 of Cx36 (Gjd2) a loxP sites-flanked construct is inserted containing both exons and the intron of Cx36 and a frt sites-flanked neomycin selection cassette. After cre recombinase activity a cyan fluorescent protein reporter gene is expressed under the control of the endogenous Cx36 promoter. |
Phenotypic information | No major defects were observed in the floxed state. After cre rembinase-mediated deletion homozygous Cx36 knock-out mice show visual transmission defects and reduced gamma oscillation. |
Breeding history | After blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown coat coloured offspring. This brown offspring was backcrossed to C57BL/6NCrl more than three times. |
References |
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Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Breeding at archiving centre | Backcrossed to C57BL/6J |
Disease and phenotype information
MGI phenotypes (allele matching)
MGI phenotypes (gene matching)
- abnormal cued conditioning behavior / MGI
- abnormal CNS synaptic transmission / MGI
- abnormal nervous system electrophysiology / MGI
- abnormal inhibitory postsynaptic potential / MGI
- abnormal excitatory postsynaptic potential / MGI
- no phenotypic analysis / MGI
- decreased insulin secretion / MGI
- abnormal single cell response / MGI
- abnormal nerve fiber response / MGI
- abnormal pancreatic beta cell physiology / MGI
- abnormal insulin secretion / MGI
- nervous system phenotype / MGI
- abnormal brain wave pattern / MGI
- abnormal retinal ganglion layer morphology / MGI
- endocrine/exocrine gland phenotype / MGI
- digestive/alimentary phenotype / MGI
- behavior/neurological phenotype / MGI
- vision/eye phenotype / MGI
- abnormal action potential / MGI
Literature references
- A new conditional mouse mutant reveals specific expression and functions of connexin36 in neurons and pancreatic beta-cells.;Wellershaus Kerstin, Degen Joachim, Deuchars Jim, Theis Martin, Charollais Anne, Caille Dorothée, Gauthier Benoit, Janssen-Bienhold Ulrike, Sonntag Stephan, Herrera Pedro, Meda Paolo, Willecke Klaus, ;2008;Experimental cell research;314;997-1012; 18258229
- Amacrine Cells Forming Gap Junctions With Intrinsically Photosensitive Retinal Ganglion Cells: ipRGC Types, Neuromodulator Contents, and Connexin Isoform.;Harrison Krystal R, Chervenak Andrew P, Resnick Sarah M, Reifler Aaron N, Wong Kwoon Y, ;2021;Investigative ophthalmology & visual science;62;10; 33410914
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