B6.129S2-Nr1d1^tm1Schb/Cnrm

Status	Available to order
EMMA ID	EM:02487
Citation information	RRID:IMSR_EM:02487 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129S2-Nr1d1^tm1Schb/Cnrm
Alternative name	REV-ERBalpha-KO
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Nr1d1^tm1Schb
Gene/Transgene symbol	Nr1d1

Information from provider

Provider	Ueli Schibler
Provider affiliation	University of Geneva
Genetic information	A genomic fragment containing part of exon 2, exons 3 and 4 and part of exon 5 was replaced with an in-frame lacZ gene and a neomycin selection cassette. Northern blot analysis demonstrated that no detectable transcript is expressed from this allele, and Western blot analysis confirmed that no detectable protein is made from this allele.
Phenotypic information	Homozygotes for targeted null mutations exhibit altered circadian behavior and mild alterations in cytoarchitecture of the cerebellum. Litter size is reduced by half in mutant females.
Breeding history	Backcrossed to C57BL/6J background for more than 6 generations.
References	The orphan nuclear receptor REV-ERBalpha controls circadian transcription within the positive limb of the mammalian circadian oscillator.;Preitner Nicolas, Damiola Francesca, Lopez-Molina Luis, Zakany Joszef, Duboule Denis, Albrecht Urs, Schibler Ueli, ;2002;Cell;110;251-60; 12150932
Homozygous fertile	no
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

IMPC phenotypes (gene matching)

abnormal retina inner nuclear layer morphology / IMPC
dysplasia / IMPC
enlarged heart / IMPC
decreased NK cell number / IMPC
increased mean corpuscular volume / IMPC
decreased total retina thickness / IMPC
unresponsive to tactile stimuli / IMPC
decreased total body fat amount / IMPC
preweaning lethality, incomplete penetrance / IMPC
abnormal eye posterior chamber depth / IMPC
increased bone mineral content / IMPC
decreased circulating alkaline phosphatase level / IMPC
increased circulating iron level / IMPC
decreased hemoglobin content / IMPC
abnormal eye anterior chamber depth / IMPC
abnormal eye morphology / IMPC
decreased cardiac output / IMPC
metaplasia / IMPC
decreased thigmotaxis / IMPC
hyperactivity / IMPC

MGI phenotypes (allele matching)

decreased litter size / MGI
shortened circadian behavior period / MGI
advanced circadian behavior phase / MGI
increased circulating LDL cholesterol level / MGI
increased circulating HDL cholesterol level / MGI
abnormal bile salt homeostasis / MGI
decreased liver triglyceride level / MGI
decreased liver cholesterol level / MGI

MGI phenotypes (gene matching)

increased circulating LDL cholesterol level / MGI
abnormal cerebellum morphology / MGI
abnormal cerebellum development / MGI
abnormal cerebellum external granule cell layer morphology / MGI
abnormal cerebellar molecular layer / MGI
decreased body weight / MGI
increased circulating HDL cholesterol level / MGI
reduced male fertility / MGI
reduced female fertility / MGI
decreased litter size / MGI
no phenotypic analysis / MGI
abnormal cerebellar granule cell morphology / MGI
increased oxygen consumption / MGI
abnormal bile salt homeostasis / MGI
increased body temperature / MGI
abnormal Purkinje cell dendrite morphology / MGI
abnormal Purkinje cell differentiation / MGI
decreased liver triglyceride level / MGI
increased skeletal muscle fiber size / MGI
abnormal skeletal muscle fiber type ratio / MGI
decreased sensitivity to induced morbidity/mortality / MGI
decreased liver cholesterol level / MGI
increased core body temperature / MGI
abnormal circadian temperature homeostasis / MGI
postnatal lethality, incomplete penetrance / MGI
shortened circadian behavior period / MGI
advanced circadian behavior phase / MGI
decreased susceptibility to induced hypothermia / MGI
increased adipocyte glucose uptake / MGI

Literature references

The orphan nuclear receptor REV-ERBalpha controls circadian transcription within the positive limb of the mammalian circadian oscillator.;Preitner Nicolas, Damiola Francesca, Lopez-Molina Luis, Zakany Joszef, Duboule Denis, Albrecht Urs, Schibler Ueli, ;2002;Cell;110;251-60; 12150932
TH17 cell differentiation is regulated by the circadian clock.;Yu Xiaofei, Rollins Darcy, Ruhn Kelly A, Stubblefield Jeremy J, Green Carla B, Kashiwada Masaki, Rothman Paul B, Takahashi Joseph S, Hooper Lora V, ;2013;Science (New York, N.Y.);342;727-30; 24202171

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

B6.129S2-Nr1d1tm1Schb/Cnrm