B6;129S7-Bdkrb2^tm1Jfh/Cnrm

Status	Available to order
EMMA ID	EM:02483
Citation information	RRID:IMSR_EM:02483 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;129S7-Bdkrb2^tm1Jfh/Cnrm
Alternative name	B2 knockout
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Bdkrb2^tm1Jfh
Gene/Transgene symbol	Bdkrb2

Information from provider

Provider	J. Fred Hess
Provider affiliation	Department of Neuroscience Drug Discovery, Merck Research Laboratories
Genetic information	Targeting construct is derived from 129/SvEvJ DNA. The coding sequence of the B2 (Bdkrb2) receptor, which lies on a single exon (exon 4), was replaced by a PGKneo cassette.
Phenotypic information	Adult and juvenile B2 (Bdkrb2) knockout animals are indistinguishable from wild type littermates by visual inspection. No abnormalities were seen in either the gross pathology or the histopathology.
Breeding history	The animals were backcrossed on a C57BL/6J background.
References	Targeted disruption of a B2 bradykinin receptor gene in mice eliminates bradykinin action in smooth muscle and neurons.;Borkowski J A, Ransom R W, Seabrook G R, Trumbauer M, Chen H, Hill R G, Strader C D, Hess J F, ;1995;The Journal of biological chemistry;270;13706-10; 7775424
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI phenotypes (allele matching)

decreased bone mineral density / MGI
abnormal kidney cortex morphology / MGI
kidney cortex cysts / MGI
abnormal kidney development / MGI
premature death / MGI
abnormal kidney morphology / MGI
abnormal kidney physiology / MGI
abnormal renal tubule morphology / MGI
dilated renal tubules / MGI
abnormal Leydig cell morphology / MGI
abnormal juxtaglomerular apparatus morphology / MGI
abnormal nephron morphology / MGI
abnormal proximal convoluted tubule morphology / MGI
abnormal renal glomerulus morphology / MGI
renal/urinary system phenotype / MGI
homeostasis/metabolism phenotype / MGI
cardiovascular system phenotype / MGI
behavior/neurological phenotype / MGI
increased circulating creatinine level / MGI
increased blood urea nitrogen level / MGI
decreased blood urea nitrogen level / MGI
abnormal renal tubule epithelium morphology / MGI
increased sensitivity to induced morbidity/mortality / MGI
postnatal lethality, incomplete penetrance / MGI
abnormal juxtaglomerular cell morphology / MGI
abnormal glomerular capillary morphology / MGI
abnormal kidney medullary ray morphology / MGI
increased glomerular capsule space / MGI
abnormal mitochondrial chromosome morphology / MGI
decreased urine nitrite level / MGI

MGI phenotypes (gene matching)

decreased bone mineral density / MGI
abnormal kidney cortex morphology / MGI
kidney cortex cysts / MGI
abnormal kidney development / MGI
premature death / MGI
abnormal kidney morphology / MGI
abnormal kidney physiology / MGI
no abnormal phenotype detected / MGI
abnormal renal tubule morphology / MGI
dilated renal tubules / MGI
abnormal Leydig cell morphology / MGI
abnormal juxtaglomerular apparatus morphology / MGI
abnormal nephron morphology / MGI
abnormal proximal convoluted tubule morphology / MGI
abnormal renal glomerulus morphology / MGI
renal/urinary system phenotype / MGI
homeostasis/metabolism phenotype / MGI
cardiovascular system phenotype / MGI
behavior/neurological phenotype / MGI
increased circulating creatinine level / MGI
increased blood urea nitrogen level / MGI
decreased blood urea nitrogen level / MGI
abnormal renal tubule epithelium morphology / MGI
increased sensitivity to induced morbidity/mortality / MGI
postnatal lethality, incomplete penetrance / MGI
abnormal juxtaglomerular cell morphology / MGI
abnormal glomerular capillary morphology / MGI
abnormal kidney medullary ray morphology / MGI
increased glomerular capsule space / MGI
abnormal mitochondrial chromosome morphology / MGI
decreased urine nitrite level / MGI

Literature references

Targeted disruption of a B2 bradykinin receptor gene in mice eliminates bradykinin action in smooth muscle and neurons.;Borkowski J A, Ransom R W, Seabrook G R, Trumbauer M, Chen H, Hill R G, Strader C D, Hess J F, ;1995;The Journal of biological chemistry;270;13706-10; 7775424

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Example health report
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Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6;129S7-Bdkrb2tm1Jfh/Cnrm