| Status | Available to order |
| EMMA ID | EM:02431 |
| Citation information | RRID:IMSR_EM:02431 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Sp4tm1Sus/Cnrm |
| Alternative name | Sp4 knockout |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Sp4tm1Sus |
| Gene/Transgene symbol | Sp4 |
| Provider | Sjaak Philipsen |
| Provider affiliation | Genetics, Erasmus MC |
| Genetic information | A targeting vector was designed to replace sequences that encode the N-terminal activation domains of Sp4 protein (amino acids 4-557). This was obtained by replacing exons 2 and 3 of the mouse Sp4 gene by IRES-LacZ-polyA/PGK-neo sequences in the targeting vector. The lacZ gene is expressed under control of the endogenous Sp4 promoter. |
| Phenotypic information | Increased mortality in the first weeks after birth, possibly due to cardiac arrest. Infertile; the animals are not interested in reproductive activities. Heterozygote Sp4 knockouts have no known phenotype. |
| Breeding history | The line was backcrossed to C57BL/6 for more than 20 generations. |
| References |
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| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
