- spleen hypoplasia / MGI
- small testis / MGI
- postnatal growth retardation / MGI
- thymus hypoplasia / MGI
- reduced female fertility / MGI
- male infertility / MGI
- delayed vaginal opening / MGI
- small uterus / MGI
- homeostasis/metabolism phenotype / MGI
- hematopoietic system phenotype / MGI
- postnatal lethality, incomplete penetrance / MGI
B6.129P2-Sp4tm1Sus/Cnrm
Status | Available to order |
EMMA ID | EM:02431 |
International strain name | B6.129P2-Sp4tm1Sus/Cnrm |
Alternative name | Sp4 knockout |
Strain type | Targeted Mutant Strains : Knock-out |
Allele/Transgene symbol | Sp4tm1Sus |
Gene/Transgene symbol | Sp4 |
Information from provider
Provider | Sjaak Philipsen |
Provider affiliation | Genetics, Erasmus MC |
Genetic information | A targeting vector was designed to replace sequences that encode the N-terminal activation domains of Sp4 protein (amino acids 4-557). This was obtained by replacing exons 2 and 3 of the mouse Sp4 gene by IRES-LacZ-polyA/PGK-neo sequences in the targeting vector. The lacZ gene is expressed under control of the endogenous Sp4 promoter. |
Phenotypic information | Increased mortality in the first weeks after birth, possibly due to cardiac arrest. Infertile; the animals are not interested in reproductive activities. Heterozygote Sp4 knockouts have no known phenotype. |
Breeding history | The line was backcrossed to C57BL/6 for more than 20 generations. |
References |
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Information from EMMA
Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
MGI phenotypes (allele matching)
MGI phenotypes (gene matching)
- spleen hypoplasia / MGI
- abnormal hippocampus morphology / MGI
- abnormal dentate gyrus morphology / MGI
- small testis / MGI
- decreased body size / MGI
- reduced male mating frequency / MGI
- abnormal locomotor behavior / MGI
- hyperactivity / MGI
- impaired coordination / MGI
- decreased exploration in new environment / MGI
- reduced long term potentiation / MGI
- abnormal startle reflex / MGI
- irregular heartbeat / MGI
- postnatal growth retardation / MGI
- thymus hypoplasia / MGI
- reduced female fertility / MGI
- male infertility / MGI
- premature death / MGI
- gliosis / MGI
- reduced sensorimotor gating / MGI
- delayed vaginal opening / MGI
- small uterus / MGI
- decreased vertical activity / MGI
- abnormal impulse conducting system conduction / MGI
- nervous system phenotype / MGI
- impaired passive avoidance behavior / MGI
- abnormal heartbeat / MGI
- absent heartbeat / MGI
- abnormal Purkinje fiber morphology / MGI
- homeostasis/metabolism phenotype / MGI
- endocrine/exocrine gland phenotype / MGI
- cardiovascular system phenotype / MGI
- reproductive system phenotype / MGI
- hematopoietic system phenotype / MGI
- abnormal spatial reference memory / MGI
- slow postnatal weight gain / MGI
- ventricular tachycardia / MGI
- impaired contextual conditioning behavior / MGI
- impaired cued conditioning behavior / MGI
- ventricular premature beat / MGI
- atrioventricular block / MGI
- sinoatrial block / MGI
- sinus bradycardia / MGI
- postnatal lethality, incomplete penetrance / MGI
- perinatal lethality, incomplete penetrance / MGI
- impaired spatial learning / MGI
Literature references
- Complex phenotype of mice homozygous for a null mutation in the Sp4 transcription factor gene.;Göllner H, Bouwman P, Mangold M, Karis A, Braun H, Rohner I, Del Rey A, Besedovsky H O, Meinhardt A, van den Broek M, Cutforth T, Grosveld F, Philipsen S, Suske G, ;2001;Genes to cells : devoted to molecular & cellular mechanisms;6;689-97; 11532028
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