B6.129P2-Irf8^tm1Hor/Kctt

Status	Available to order
EMMA ID	EM:02414
International strain name	B6.129P2-Irf8^tm1Hor/Kctt
Alternative name	Irf8-/-
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Irf8^tm1Hor
Gene/Transgene symbol	Irf8

Information from provider

Provider	Ivan Horak
Provider affiliation	Leibniz-Institut für Molekulare Pharmakologie
Genetic information	A PGK-neomycin gene was inserted into the second exon of the Irf8 gene, disrupting sequences encoding the DNA binding domain.
Phenotypic information	Abnormal hematopoiesis.
References	Crucial role of interferon consensus sequence binding protein, but neither of interferon regulatory factor 1 nor of nitric oxide synthesis for protection against murine listeriosis.;Fehr T, Schoedon G, Odermatt B, Holtschke T, Schneemann M, Bachmann M F, Mak T W, Horak I, Zinkernagel R M, ;1997;The Journal of experimental medicine;185;921-31; 9120398 Interferon consensus sequence binding protein-deficient mice display impaired resistance to intracellular infection due to a primary defect in interleukin 12 p40 induction.;Scharton-Kersten T, Contursi C, Masumi A, Sher A, Ozato K, ;1997;The Journal of experimental medicine;186;1523-34; 9348310 Interferon (IFN) consensus sequence-binding protein, a transcription factor of the IFN regulatory factor family, regulates immune responses in vivo through control of interleukin 12 expression.;Giese N A, Gabriele L, Doherty T M, Klinman D M, Tadesse-Heath L, Contursi C, Epstein S L, Morse H C, ;1997;The Journal of experimental medicine;186;1535-46; 9348311 Lack of interferon consensus sequence binding protein (ICSBP) transcripts in human myeloid leukemias.;Schmidt M, Nagel S, Proba J, Thiede C, Ritter M, Waring J F, Rosenbauer F, Huhn D, Wittig B, Horak I, Neubauer A, ;1998;Blood;91;22-9; 9414265 Altered development and cytokine responses of myeloid progenitors in the absence of transcription factor, interferon consensus sequence binding protein.;Scheller M, Foerster J, Heyworth C M, Waring J F, Löhler J, Gilmore G L, Shadduck R K, Dexter T M, Horak I, ;1999;Blood;94;3764-71; 10572090 ICSBP is essential for the development of mouse type I interferon-producing cells and for the generation and activation of CD8alpha(+) dendritic cells.;Schiavoni Giovanna, Mattei Fabrizio, Sestili Paola, Borghi Paola, Venditti Massimo, Morse Herbert C, Belardelli Filippo, Gabriele Lucia, ;2002;The Journal of experimental medicine;196;1415-25; 12461077 AML1-ETO inhibits maturation of multiple lymphohematopoietic lineages and induces myeloblast transformation in synergy with ICSBP deficiency.;Schwieger Maike, Löhler Jürgen, Friel Jutta, Scheller Marina, Horak Ivan, Stocking Carol, ;2002;The Journal of experimental medicine;196;1227-40; 12417632 Essential role for ICSBP in the in vivo development of murine CD8alpha + dendritic cells.;Aliberti Julio, Schulz Oliver, Pennington Daniel J, Tsujimura Hideki, Reis e Sousa Caetano, Ozato Keiko, Sher Alan, ;2003;Blood;101;305-10; 12393690 Immunodeficiency and chronic myelogenous leukemia-like syndrome in mice with a targeted mutation of the ICSBP gene.;Holtschke T, Löhler J, Kanno Y, Fehr T, Giese N, Rosenbauer F, Lou J, Knobeloch K P, Gabriele L, Waring J F, Bachmann M F, Zinkernagel R M, Morse H C, Ozato K, Horak I, ;1996;Cell;87;307-17; 8861914

Information from EMMA

Archiving centre	Karolinska Institutet, Stockholm, Sweden

Disease and phenotype information

MGI allele-associated human disease models

familial chronic myelocytic leukemia-like syndrome / DOID:0060761

Orphanet associated rare diseases, based on orthologous gene matching

Mendelian susceptibility to mycobacterial diseases due to partial IRF8 deficiency / Orphanet_319600

IMPC phenotypes (gene matching)

increased circulating sodium level / IMPC
thrombocytopenia / IMPC
increased circulating total protein level / IMPC
decreased circulating free fatty acids level / IMPC
decreased fasting circulating glucose level / IMPC
decreased erythrocyte cell number / IMPC
increased mean corpuscular volume / IMPC
increased mean corpuscular hemoglobin / IMPC

MGI phenotypes (allele matching)

premature death / MGI
abnormal definitive hematopoiesis / MGI
increased chronic myelocytic leukemia incidence / MGI
increased B-1b cell number / MGI
decreased follicular B cell number / MGI
increased marginal zone B cell number / MGI
increased leukocyte cell number / MGI
abnormal erythropoiesis / MGI
increased bone marrow cell number / MGI
increased granulocyte number / MGI
enlarged liver / MGI
enlarged spleen / MGI
spleen hyperplasia / MGI
enlarged lymph nodes / MGI
abnormal megakaryocyte morphology / MGI
increased susceptibility to viral infection / MGI
increased immunoglobulin level / MGI
increased lymphocyte cell number / MGI
increased B cell number / MGI
decreased lymphocyte cell number / MGI
decreased B cell number / MGI
decreased T cell number / MGI
abnormal cytotoxic T cell physiology / MGI
increased macrophage cell number / MGI
abnormal plasma cell number / MGI
increased mature B cell number / MGI
increased spleen red pulp amount / MGI
decreased interferon-gamma secretion / MGI
increased sensitivity to induced morbidity/mortality / MGI
increased lymphoma incidence / MGI
abnormal humoral immune response / MGI

MGI phenotypes (gene matching)

increased leukocyte cell number / MGI
increased neutrophil cell number / MGI
decreased monocyte cell number / MGI
abnormal erythropoiesis / MGI
increased bone marrow cell number / MGI
increased granulocyte number / MGI
enlarged liver / MGI
enlarged spleen / MGI
spleen hyperplasia / MGI
enlarged lymph nodes / MGI
abnormal myelopoiesis / MGI
abnormal humoral immune response / MGI
premature death / MGI
abnormal definitive hematopoiesis / MGI
no abnormal phenotype detected / MGI
abnormal megakaryocyte morphology / MGI
increased susceptibility to viral infection / MGI
increased immunoglobulin level / MGI
no phenotypic analysis / MGI
decreased macrophage cell number / MGI
increased lymphocyte cell number / MGI
increased B cell number / MGI
decreased lymphocyte cell number / MGI
decreased B cell number / MGI
decreased T cell number / MGI
abnormal cytotoxic T cell physiology / MGI
increased macrophage cell number / MGI
increased chronic myelocytic leukemia incidence / MGI
abnormal plasma cell number / MGI
increased B-1b cell number / MGI
increased follicular B cell number / MGI
decreased follicular B cell number / MGI
increased marginal zone B cell number / MGI
increased transitional stage B cell number / MGI
increased mature B cell number / MGI
increased spleen red pulp amount / MGI
abnormal interferon secretion / MGI
decreased interferon-gamma secretion / MGI
increased sensitivity to induced morbidity/mortality / MGI
increased lymphoma incidence / MGI

Literature references

Crucial role of interferon consensus sequence binding protein, but neither of interferon regulatory factor 1 nor of nitric oxide synthesis for protection against murine listeriosis.;Fehr T, Schoedon G, Odermatt B, Holtschke T, Schneemann M, Bachmann M F, Mak T W, Horak I, Zinkernagel R M, ;1997;The Journal of experimental medicine;185;921-31; 9120398
Interferon consensus sequence binding protein-deficient mice display impaired resistance to intracellular infection due to a primary defect in interleukin 12 p40 induction.;Scharton-Kersten T, Contursi C, Masumi A, Sher A, Ozato K, ;1997;The Journal of experimental medicine;186;1523-34; 9348310
Interferon (IFN) consensus sequence-binding protein, a transcription factor of the IFN regulatory factor family, regulates immune responses in vivo through control of interleukin 12 expression.;Giese N A, Gabriele L, Doherty T M, Klinman D M, Tadesse-Heath L, Contursi C, Epstein S L, Morse H C, ;1997;The Journal of experimental medicine;186;1535-46; 9348311
Lack of interferon consensus sequence binding protein (ICSBP) transcripts in human myeloid leukemias.;Schmidt M, Nagel S, Proba J, Thiede C, Ritter M, Waring J F, Rosenbauer F, Huhn D, Wittig B, Horak I, Neubauer A, ;1998;Blood;91;22-9; 9414265
Altered development and cytokine responses of myeloid progenitors in the absence of transcription factor, interferon consensus sequence binding protein.;Scheller M, Foerster J, Heyworth C M, Waring J F, Löhler J, Gilmore G L, Shadduck R K, Dexter T M, Horak I, ;1999;Blood;94;3764-71; 10572090
ICSBP is essential for the development of mouse type I interferon-producing cells and for the generation and activation of CD8alpha(+) dendritic cells.;Schiavoni Giovanna, Mattei Fabrizio, Sestili Paola, Borghi Paola, Venditti Massimo, Morse Herbert C, Belardelli Filippo, Gabriele Lucia, ;2002;The Journal of experimental medicine;196;1415-25; 12461077
AML1-ETO inhibits maturation of multiple lymphohematopoietic lineages and induces myeloblast transformation in synergy with ICSBP deficiency.;Schwieger Maike, Löhler Jürgen, Friel Jutta, Scheller Marina, Horak Ivan, Stocking Carol, ;2002;The Journal of experimental medicine;196;1227-40; 12417632
Essential role for ICSBP in the in vivo development of murine CD8alpha + dendritic cells.;Aliberti Julio, Schulz Oliver, Pennington Daniel J, Tsujimura Hideki, Reis e Sousa Caetano, Ozato Keiko, Sher Alan, ;2003;Blood;101;305-10; 12393690
Immunodeficiency and chronic myelogenous leukemia-like syndrome in mice with a targeted mutation of the ICSBP gene.;Holtschke T, Löhler J, Kanno Y, Fehr T, Giese N, Rosenbauer F, Lou J, Knobeloch K P, Gabriele L, Waring J F, Bachmann M F, Zinkernagel R M, Morse H C, Ozato K, Horak I, ;1996;Cell;87;307-17; 8861914
Interferon regulatory factor 8 protects against cerebral ischaemic-reperfusion injury.;Xiang Mei, Wang Lang, Guo Sen, Lu Yan-Yun, Lei Hao, Jiang Ding-Sheng, Zhang Yan, Liu Yi, Zhou Yan, Zhang Xiao-Dong, Li Hongliang, ;2014;Journal of neurochemistry;129;988-1001; 24528256
IRF8 suppresses pathological cardiac remodelling by inhibiting calcineurin signalling.;Jiang Ding-Sheng, Wei Xiang, Zhang Xiao-Fei, Liu Yu, Zhang Yan, Chen Ke, Gao Lu, Zhou Heng, Zhu Xue-Hai, Liu Peter P, Bond Lau Wayne, Ma Xinliang, Zou Yunzeng, Zhang Xiao-Dong, Fan Guo-Chang, Li Hongliang, ;2014;Nature communications;5;3303; 24526256
Interferon regulatory factor 8 modulates phenotypic switching of smooth muscle cells by regulating the activity of myocardin.;Zhang Shu-Min, Gao Lu, Zhang Xiao-Fei, Zhang Ran, Zhu Li-Hua, Wang Pi-Xiao, Tian Song, Yang Da, Chen Ke, Huang Ling, Zhang Xiao-Dong, Li Hongliang, ;2014;Molecular and cellular biology;34;400-14; 24248596

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6.129P2-Irf8tm1Hor/Kctt