| Status | Available to order |
| EMMA ID | EM:02384 |
| Citation information | RRID:IMSR_EM:02384 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2(Cg)-Gjb3tm2.2Kwi/Cnrm |
| Alternative name | Cx31floxdNCx31F137L |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Gjb3tm2.2Kwi |
| Gene/Transgene symbol | Gjb3 |
| Provider | Klaus WILLECKE |
| Provider affiliation | Molekulargenetik, Institut fuer Genetik, Universitaet Bonn |
| Genetic information | After cre recombinase-mediated deletion of the Cx31 (Gjb3) coding region, the point mutation Cx31-F137L is expressed under the control of the endogenous Cx31 promoter. One frt site between the two loxP sites is remaining in the genome, upon deletion of the frt sites-flanked neomycin-selection cassette. |
| Phenotypic information | Not in the loxP flanked state. After ubiquitous expression of Cx31-F137L, heterozygous mice have skin abnormalities and homozygous mice are not viable. |
| Breeding history | After blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown offspring. This brown offspring was backcrossed once with flp recombinase deleter mice (nearly 100% C57BL/6NCrl) to delete the selection cassette and after that more than three times with C57BL/6NCrl. |
| References |
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| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Backcrossed to C57BL/6J |
Orphanet associated rare diseases, based on orthologous gene matching
