MGI phenotypes (allele matching)
B6;Cg-Gjb5tm1Kwi/Cnrm
| Status | Available to order |
| EMMA ID | EM:02382 |
| Citation information | RRID:IMSR_EM:02382 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;Cg-Gjb5tm1Kwi/Cnrm |
| Alternative name | Cx31.1LacZ |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Gjb5tm1Kwi |
| Gene/Transgene symbol | Gjb5 |
Information from provider
| Provider | Klaus WILLECKE |
| Provider affiliation | Molekulargenetik, Institut fuer Genetik, Universitaet Bonn |
| Genetic information | Instead of the whole open reading frame of exon 2 of Cx31.1 (Gjb5), the coding region of the reporter gene LacZ is inserted. One loxP site, 3' of the poly(A)-signal, is remaining in the genome, upon deletion of the loxP-flanked HPRT-selection cassette. |
| Phenotypic information | Reduced knock-out survival. |
| Breeding history | After blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown offspring. This brown offspring was backcrossed once with pgk-cre mice (nearly 100% C57BL/6NCrl) to delete the selection cassette and after that more than two times with C57BL/6NCrl. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Backcrossed to C57BL/6J |
Disease and phenotype information
Literature references
- Characterization of connexin31.1-deficient mice reveals impaired placental development.;Zheng-Fischhöfer Qingyi, Kibschull Mark, Schnichels Marc, Kretz Markus, Petrasch-Parwez Elisabeth, Strotmann Jörg, Reucher Harald, Lynn Bruce D, Nagy James I, Lye Stephen J, Winterhager Elke, Willecke Klaus, ;2007;Developmental biology;312;258-71; 17961533