B6.129S2-Atp1b2tm1Msch/Cnrm

Status

Available to order

EMMA IDEM:00023
Citation informationRRID:IMSR_EM:00023 

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International strain nameB6.129S2-Atp1b2tm1Msch/Cnrm
Alternative nameAMOG-KO
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolAtp1b2tm1Msch
Gene/Transgene symbolAtp1b2

Information from provider

ProviderMelitta Schachner
Provider affiliationCenter for molecular neurobiology Hamburg, Center for molecular neurobiology Hamburg
Genetic informationThe neo gene was inserted into the first exon of the Atp1b2 (AMOG/2) genomic clone G7sH resulting in two in-frame stop codons.
Phenotypic informationHomozygous animals die 17-18 d after birth. They display motor uncoordination at 15 d of age. Pathological traits of the nervous system (17-18 d after birth): enlarged ventricles, degenerating photoreceptor cells, swelling and degeneration of astrocytic endfeet in brain stem.
Breeding historyBackcrossed to C57BL/6 (more than 5 generations).
References
  • Degeneration of neural cells in the central nervous system of mice deficient in the gene for the adhesion molecule on Glia, the beta 2 subunit of murine Na,K-ATPase.;Magyar J P, Bartsch U, Wang Z Q, Howells N, Aguzzi A, Wagner E F, Schachner M, ;1994;The Journal of cell biology;127;835-45; 7525597

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy
Animals used for archivingheterozygous C57BL/6 males, wild-type C57BL/6 females
Stage of embryos2-cell

Disease and phenotype information

MGI phenotypes (allele matching)
  • tremors / MGI
  • forelimb paralysis / MGI
  • abnormal cerebellar granule layer morphology / MGI
  • abnormal retinal photoreceptor morphology / MGI
  • retinal degeneration / MGI
  • impaired coordination / MGI
  • impaired righting response / MGI
  • nervous system phenotype / MGI
  • eyelids fail to open / MGI
  • abnormal brainstem morphology / MGI
  • vision/eye phenotype / MGI
  • abnormal food intake / MGI
  • retinal photoreceptor degeneration / MGI
  • thin retinal outer nuclear layer / MGI
  • retinal outer nuclear layer degeneration / MGI
  • enlarged lateral ventricles / MGI
  • enlarged third ventricle / MGI
  • short photoreceptor inner segment / MGI
  • short photoreceptor outer segment / MGI
  • postnatal lethality, complete penetrance / MGI
MGI phenotypes (gene matching)
  • tremors / MGI
  • forelimb paralysis / MGI
  • abnormal cerebellar granule layer morphology / MGI
  • abnormal retinal photoreceptor morphology / MGI
  • retinal degeneration / MGI
  • impaired coordination / MGI
  • impaired righting response / MGI
  • nervous system phenotype / MGI
  • eyelids fail to open / MGI
  • abnormal brainstem morphology / MGI
  • vision/eye phenotype / MGI
  • abnormal food intake / MGI
  • abnormal retinal neuronal layer morphology / MGI
  • retinal photoreceptor degeneration / MGI
  • thin retinal outer nuclear layer / MGI
  • retinal outer nuclear layer degeneration / MGI
  • enlarged lateral ventricles / MGI
  • enlarged third ventricle / MGI
  • short photoreceptor inner segment / MGI
  • short photoreceptor outer segment / MGI
  • postnatal lethality, complete penetrance / MGI
  • increased retinal apoptosis / MGI

Literature references

  • Degeneration of neural cells in the central nervous system of mice deficient in the gene for the adhesion molecule on Glia, the beta 2 subunit of murine Na,K-ATPase.;Magyar J P, Bartsch U, Wang Z Q, Howells N, Aguzzi A, Wagner E F, Schachner M, ;1994;The Journal of cell biology;127;835-45; 7525597

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

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Practical information

Genotyping protocol

Example health report
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Material Transfer Agreement (MTA)
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Legally binding conditions for the transfer

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