B6.129-Tgm2^tm1Gml/Cnrm

Status	Available to order
EMMA ID	EM:02283
International strain name	B6.129-Tgm2^tm1Gml/Cnrm
Alternative name	TG2KO
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Tgm2^tm1Gml
Gene/Transgene symbol	Tgm2

Information from provider

Provider	Gerry Melino
Provider affiliation	University of Tor Vergata
Genetic information	The targeting vector was constructed by cloning an ~4-kb EcoRV/BamHI fragment containing the sequence from intron 6 to exon 9, into the pPNT vector, 3' of the neomycin resistance gene between the XbaI and BamHI unique sites. An ~2-kb fragment containing intron 3 was generated by genotyping using primers designed on the basis of the sequence of exons 4 and 5. This fragment was cloned into the XhoI site of the pPNT vector 5' of the neomycin resistance gene. This construct deletes 1.2 kb containing part of exon 5, intron 5, exon 6, and a small piece of intron 6 up to the EcoRV site.
Phenotypic information	Defects in glucose tolerance; defects on phagocytosis-associated crosstalk between macrophages and apoptotic cells; defective function of mitochondrial respiratory complex I.
Breeding history	Backcrossed for 10 generations.
References	Role of transglutaminase 2 in glucose tolerance: knockout mice studies and a putative mutation in a MODY patient.;Bernassola Francesca, Federici Massimo, Corazzari Marco, Terrinoni Alessandro, Hribal Marta L, De Laurenzi Vincenzo, Ranalli Marco, Massa Ornella, Sesti Giorgio, McLean W H Irwin, Citro Gennaro, Barbetti Fabrizio, Melino Gerry, ;2002;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;16;1371-8; 12205028 Transglutaminase 2-/- mice reveal a phagocytosis-associated crosstalk between macrophages and apoptotic cells.;Szondy Zsuzsa, Sarang Zsolt, Molnar Peter, Nemeth Tamas, Piacentini Mauro, Mastroberardino Pier Giorgio, Falasca Laura, Aeschlimann Daniel, Kovacs Judit, Kiss Ildiko, Szegezdi Eva, Lakos Gabriella, Rajnavolgyi Eva, Birckbichler Paul J, Melino Gerry, Fesus Laszlo, ;2003;Proceedings of the National Academy of Sciences of the United States of America;100;7812-7; 12810961 Transglutaminase 2 ablation leads to defective function of mitochondrial respiratory complex I affecting neuronal vulnerability in experimental models of extrapyramidal disorders.;Battaglia Giuseppe, Farrace Maria Grazia, Mastroberardino Pier Giorgio, Viti Irene, Fimia Gian Maria, Van Beeumen Jozef, Devreese Bart, Melino Gennaro, Molinaro Gemma, Busceti Carla Letizia, Biagioni Francesca, Nicoletti Ferdinando, Piacentini Mauro, ;2007;Journal of neurochemistry;100;36-49; 17064362

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI allele-associated human disease models

maturity-onset diabetes of the young / DOID:0050524

MGI phenotypes (allele matching)

altered response to myocardial infarction / MGI
abnormal cardiovascular system physiology / MGI
premature death / MGI
disorganized myocardium / MGI
increased myocardial infarction size / MGI
increased tumor growth/size / MGI
renal/urinary system phenotype / MGI
cardiovascular system phenotype / MGI
immune system phenotype / MGI
increased macrophage cell number / MGI
ventricular fibrillation / MGI
decreased susceptibility to endotoxin shock / MGI
decreased sensitivity to induced morbidity/mortality / MGI
abnormal mitochondrial ATP synthesis coupled electron transport / MGI
decreased hematocrit / MGI
hyperglycemia / MGI
decreased circulating insulin level / MGI
decreased erythrocyte cell number / MGI
increased insulin sensitivity / MGI
impaired glucose tolerance / MGI
increased physiological sensitivity to xenobiotic / MGI
increased sensitivity to induced morbidity/mortality / MGI
abnormal thymus morphology / MGI
impaired macrophage phagocytosis / MGI
homeostasis/metabolism phenotype / MGI

MGI phenotypes (gene matching)

decreased hematocrit / MGI
altered response to myocardial infarction / MGI
abnormal thymus morphology / MGI
increased body weight / MGI
abnormal cardiovascular system physiology / MGI
hyperglycemia / MGI
impaired macrophage phagocytosis / MGI
premature death / MGI
disorganized myocardium / MGI
abnormal T cell physiology / MGI
decreased circulating insulin level / MGI
decreased erythrocyte cell number / MGI
increased insulin sensitivity / MGI
no phenotypic analysis / MGI
increased myocardial infarction size / MGI
abnormal cell adhesion / MGI
increased tumor growth/size / MGI
decreased susceptibility to injury / MGI
impaired glucose tolerance / MGI
renal/urinary system phenotype / MGI
homeostasis/metabolism phenotype / MGI
growth/size/body region phenotype / MGI
cardiovascular system phenotype / MGI
immune system phenotype / MGI
increased macrophage cell number / MGI
ventricular fibrillation / MGI
decreased susceptibility to endotoxin shock / MGI
increased physiological sensitivity to xenobiotic / MGI
increased sensitivity to induced morbidity/mortality / MGI
decreased sensitivity to induced morbidity/mortality / MGI
abnormal mitochondrial ATP synthesis coupled electron transport / MGI

Literature references

Role of transglutaminase 2 in glucose tolerance: knockout mice studies and a putative mutation in a MODY patient.;Bernassola Francesca, Federici Massimo, Corazzari Marco, Terrinoni Alessandro, Hribal Marta L, De Laurenzi Vincenzo, Ranalli Marco, Massa Ornella, Sesti Giorgio, McLean W H Irwin, Citro Gennaro, Barbetti Fabrizio, Melino Gerry, ;2002;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;16;1371-8; 12205028
Transglutaminase 2-/- mice reveal a phagocytosis-associated crosstalk between macrophages and apoptotic cells.;Szondy Zsuzsa, Sarang Zsolt, Molnar Peter, Nemeth Tamas, Piacentini Mauro, Mastroberardino Pier Giorgio, Falasca Laura, Aeschlimann Daniel, Kovacs Judit, Kiss Ildiko, Szegezdi Eva, Lakos Gabriella, Rajnavolgyi Eva, Birckbichler Paul J, Melino Gerry, Fesus Laszlo, ;2003;Proceedings of the National Academy of Sciences of the United States of America;100;7812-7; 12810961
Transglutaminase 2 ablation leads to defective function of mitochondrial respiratory complex I affecting neuronal vulnerability in experimental models of extrapyramidal disorders.;Battaglia Giuseppe, Farrace Maria Grazia, Mastroberardino Pier Giorgio, Viti Irene, Fimia Gian Maria, Van Beeumen Jozef, Devreese Bart, Melino Gennaro, Molinaro Gemma, Busceti Carla Letizia, Biagioni Francesca, Nicoletti Ferdinando, Piacentini Mauro, ;2007;Journal of neurochemistry;100;36-49; 17064362
Niche-specific requirement for hyphal wall protein 1 in virulence of Candida albicans.;Staab Janet F, Datta Kausik, Rhee Peter, ;2013;PloS one;8;e80842; 24260489

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6.129-Tgm2tm1Gml/Cnrm