B6N;D2-Tg(S100b-EGFP)11Lgrv/Orl
| Status | Available to order |
| EMMA ID | EM:02272 |
| Citation information | RRID:IMSR_EM:02272 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6N;D2-Tg(S100b-EGFP)11Lgrv/Orl |
| Alternative name | pS100B-EGFP |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(S100b-EGFP)11Lgrv |
| Gene/Transgene symbol | Tg(S100b-EGFP)11Lgrv |
Information from provider
| Provider | Catherine Legraverend |
| Provider affiliation | institut de genomique fonctionnelle |
| Genetic information | A 4.8Kb S100B promoter-containing sequence (-1669/+3106) was derived from Swiss mouse genomic DNA by genotyping using the Expand Long Template genotyping System (Boehringer Mannheim, Germany). The 4.8Kb S100B genomic fragment was subcloned in the TA-cloning pGEM-T Easy Vector (Promega, Madison, WI), retrieved by EcoRI digestion and finally ligated into the pEGFP-1 promoterless expression vector (Clontech Laboratories, Palo Alto, CA) and entirely sequenced on both strands by the method of Sanger. The pBG transgene was excised by double BglII/AflII digestion, electrophoresed on agarose and extracted-purified with a WIZARD kit (Promega). |
| Phenotypic information | All pS100B-EGFP mice are fertile and showed no apparent abnormality in development or behavior upon visual inspection at up to 12 months of age. |
| Breeding history | Fertilized (C57BL/6N x DBA/2N) pronuclear-stage embryos were microinjected at the S.E.A.T transgenic facilities of the CNRS (Villejuif, France). Four founders were back-crossed to C57BL/6N mice. |
| References |
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Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
Literature references
- Visualization of S100B-positive neurons and glia in the central nervous system of EGFP transgenic mice.;Vives Virginie, Alonso Gérard, Solal Anne Cohen, Joubert Dominique, Legraverend Catherine, ;2003;The Journal of comparative neurology;457;404-19; 12561079
- Spatial and temporal expression of S100B in cells of oligodendrocyte lineage.;Hachem S, Aguirre A, Vives V, Marks A, Gallo V, Legraverend C, ;2005;Glia;51;81-97; 15782413
- S100B expression defines a state in which GFAP-expressing cells lose their neural stem cell potential and acquire a more mature developmental stage.;Raponi Eric, Agenes Fabien, Delphin Christian, Assard Nicole, Baudier Jacques, Legraverend Catherine, Deloulme Jean-Christophe, ;2007;Glia;55;165-77; 17078026
- Expression of S100B during embryonic development of the mouse cerebellum.;Hachem Sabira, Laurenson Anne-Sophie, Hugnot Jean-Philippe, Legraverend Catherine, ;2007;BMC developmental biology;7;17; 17362503