B6N;D2-Tg(S100b-EGFP)11Lgrv/Orl
| Status | Available to order |
| EMMA ID | EM:02272 |
| International strain name | B6N;D2-Tg(S100b-EGFP)11Lgrv/Orl |
| Alternative name | pS100B-EGFP |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(S100b-EGFP)11Lgrv |
| Gene/Transgene symbol | Tg(S100b-EGFP)11Lgrv |
Information from provider
| Provider | Catherine Legraverend |
| Provider affiliation | institut de genomique fonctionnelle |
| Genetic information | A 4.8Kb S100B promoter-containing sequence (-1669/+3106) was derived from Swiss mouse genomic DNA by genotyping using the Expand Long Template genotyping System (Boehringer Mannheim, Germany). The 4.8Kb S100B genomic fragment was subcloned in the TA-cloning pGEM-T Easy Vector (Promega, Madison, WI), retrieved by EcoRI digestion and finally ligated into the pEGFP-1 promoterless expression vector (Clontech Laboratories, Palo Alto, CA) and entirely sequenced on both strands by the method of Sanger. The pBG transgene was excised by double BglII/AflII digestion, electrophoresed on agarose and extracted-purified with a WIZARD kit (Promega). |
| Phenotypic information | All pS100B-EGFP mice are fertile and showed no apparent abnormality in development or behavior upon visual inspection at up to 12 months of age. |
| Breeding history | Fertilized (C57BL/6N x DBA/2N) pronuclear-stage embryos were microinjected at the S.E.A.T transgenic facilities of the CNRS (Villejuif, France). Four founders were back-crossed to C57BL/6N mice. |
| References |
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Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
Literature references
- Visualization of S100B-positive neurons and glia in the central nervous system of EGFP transgenic mice.;Vives Virginie, Alonso Gérard, Solal Anne Cohen, Joubert Dominique, Legraverend Catherine, ;2003;The Journal of comparative neurology;457;404-19; 12561079
- Spatial and temporal expression of S100B in cells of oligodendrocyte lineage.;Hachem S, Aguirre A, Vives V, Marks A, Gallo V, Legraverend C, ;2005;Glia;51;81-97; 15782413
- S100B expression defines a state in which GFAP-expressing cells lose their neural stem cell potential and acquire a more mature developmental stage.;Raponi Eric, Agenes Fabien, Delphin Christian, Assard Nicole, Baudier Jacques, Legraverend Catherine, Deloulme Jean-Christophe, ;2007;Glia;55;165-77; 17078026
- Expression of S100B during embryonic development of the mouse cerebellum.;Hachem Sabira, Laurenson Anne-Sophie, Hugnot Jean-Philippe, Legraverend Catherine, ;2007;BMC developmental biology;7;17; 17362503