C57BL/6JSfdAnu-Card11unm/ApbH

Status

Available to order

EMMA IDEM:02174
International strain nameC57BL/6JSfdAnu-Card11unm/ApbH
Alternative nameUnmodulated, C57BL/6Apb-Card11/Apb
Strain typeInduced Mutant Strains : Chemically-induced
Allele/Transgene symbolCard11unm
Gene/Transgene symbolCard11

Information from provider

ProviderChris Goodnow
Provider affiliationThe Australian National University
Genetic informationThis strain carries a T to A (L305Q) point mutation. Functional assays involving serological tests and two-step protein immunization have identified strains with defects in autoimmunity, changes in T cell help for antibody, Th1/Th2 antibody polarization, humoral memory and affinity maturation, and T cell-independent antibody responses. This led to the discovery of a key regulator of antibody responses and T cell costimulation, Card11/Carma1, where a single amino acid substitution revealed separate roles in lymphocyte activation and in immunoregulation. In the missense strain, defects in the latter lead to atopic dermatitis / hyper-IgE disease, in contrast to knockout mice analysed in parallel which only show the lymphocyte activation roles of this key signal-scaffolding molecule.
Phenotypic informationThis strain carries B and T cell response defects; atopic dermatitis; high IgM on IgD+ cells, also slightly increased CD21; reduced peritoneal B1 cells; severely affected immunity mediated by Th1 cells, selective defects in BCR and TCR signalling to JNK and NFkB. The mice make poor antibody responses, and as they age develop atopic dermatitis and hyper-IgE, with red itchy ears weepy eyes, progressing to badly eroded skin from itching, at which point they need to be put down.
References
  • Identifying the MAGUK protein Carma-1 as a central regulator of humoral immune responses and atopy by genome-wide mouse mutagenesis.;Jun Jesse E, Wilson Lauren E, Vinuesa Carola G, Lesage Sylvie, Blery Mathieu, Miosge Lisa A, Cook Matthew C, Kucharska Edyta M, Hara Hiromitsu, Penninger Josef M, Domashenz Heather, Hong Nancy A, Glynne Richard J, Nelms Keats A, Goodnow Christopher C, ;2003;Immunity;18;751-62; 12818157

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (gene matching)
  • small spleen / IMPC
  • abnormal spleen morphology / IMPC
  • increased T cell number / IMPC
  • small testis / IMPC
  • abnormal testis morphology / IMPC
  • decreased effector memory CD8-positive, alpha-beta T cell number / IMPC
  • abnormal thymus morphology / IMPC
  • increased T-helper cell number / IMPC
  • decreased CD4-positive, CD25-positive, alpha-beta regulatory T cell number / IMPC
  • decreased spleen weight / IMPC
  • decreased effector memory T-helper cell number / IMPC
  • increased freezing behavior / IMPC
  • increased CD4-positive, alpha-beta T cell number / IMPC
  • increased CD8-positive, naive alpha-beta T cell number / IMPC
  • increased CD8-positive, alpha-beta T cell number / IMPC
  • enlarged thymus / IMPC
  • increased lymphocyte cell number / IMPC
  • decreased locomotor activity / IMPC
  • increased leukocyte cell number / IMPC
  • hydrometra / IMPC
  • abnormal uterus morphology / IMPC
  • decreased circulating alkaline phosphatase level / IMPC
MGI phenotypes (allele matching)
  • spleen hyperplasia / MGI
  • dermatitis / MGI
  • abnormal immune system physiology / MGI
  • decreased IgM level / MGI
  • abnormal T cell activation / MGI
  • increased inflammatory response / MGI
  • abnormal B cell differentiation / MGI
  • decreased immunoglobulin level / MGI
  • increased immunoglobulin level / MGI
  • increased IgE level / MGI
  • erythroderma / MGI
  • decreased B-1 B cell number / MGI
  • lymph node hyperplasia / MGI
  • decreased IgG3 level / MGI
MGI phenotypes (gene matching)
  • spleen hyperplasia / MGI
  • dermatitis / MGI
  • abnormal immune system physiology / MGI
  • decreased IgG level / MGI
  • decreased IgM level / MGI
  • decreased IgA level / MGI
  • abnormal T cell activation / MGI
  • increased inflammatory response / MGI
  • lung inflammation / MGI
  • abnormal B cell differentiation / MGI
  • abnormal T cell differentiation / MGI
  • decreased airway responsiveness / MGI
  • abnormal double-negative T cell morphology / MGI
  • abnormal B cell physiology / MGI
  • decreased immunoglobulin level / MGI
  • increased immunoglobulin level / MGI
  • decreased IgE level / MGI
  • increased IgE level / MGI
  • abnormal lymphocyte morphology / MGI
  • abnormal cytokine secretion / MGI
  • erythroderma / MGI
  • decreased regulatory T cell number / MGI
  • increased B-1 B cell number / MGI
  • decreased B-1 B cell number / MGI
  • abnormal cytotoxic T cell physiology / MGI
  • decreased B cell proliferation / MGI
  • abnormal T cell proliferation / MGI
  • decreased T cell proliferation / MGI
  • abnormal B cell proliferation / MGI
  • immune system phenotype / MGI
  • decreased NK T cell number / MGI
  • decreased NK cell number / MGI
  • decreased memory T cell number / MGI
  • increased CD4-positive, alpha beta T cell number / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • abnormal T-helper 2 cell differentiation / MGI
  • lymph node hyperplasia / MGI
  • increased B-1a cell number / MGI
  • decreased B-1a cell number / MGI
  • increased B-1b cell number / MGI
  • absent B-1a cells / MGI
  • decreased mature B cell number / MGI
  • absent immature B cells / MGI
  • decreased gamma-delta T cell number / MGI
  • abnormal CD4-positive, CD25-positive, alpha-beta regulatory T cell morphology / MGI
  • decreased IgG1 level / MGI
  • decreased IgG2a level / MGI
  • decreased IgG2b level / MGI
  • decreased IgG3 level / MGI
  • decreased interferon-gamma secretion / MGI
  • decreased circulating interleukin-4 level / MGI
  • decreased circulating interleukin-5 level / MGI
  • abnormal interleukin-2 secretion / MGI
  • decreased interleukin-2 secretion / MGI
  • decreased IgG2c level / MGI
  • abnormal NK cell physiology / MGI
  • impaired humoral immune response / MGI

Literature references

  • Identifying the MAGUK protein Carma-1 as a central regulator of humoral immune responses and atopy by genome-wide mouse mutagenesis.;Jun Jesse E, Wilson Lauren E, Vinuesa Carola G, Lesage Sylvie, Blery Mathieu, Miosge Lisa A, Cook Matthew C, Kucharska Edyta M, Hara Hiromitsu, Penninger Josef M, Domashenz Heather, Hong Nancy A, Glynne Richard J, Nelms Keats A, Goodnow Christopher C, ;2003;Immunity;18;751-62; 12818157

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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