B6.129P2-Nr3c1^tm2Gsc/Ieg

Status	Available to order
EMMA ID	EM:02124
International strain name	B6.129P2-Nr3c1^tm2Gsc/Ieg
Alternative name	GRflox
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Nr3c1^tm2Gsc
Gene/Transgene symbol	Nr3c1

Information from provider

Provider	Günther Schütz
Provider affiliation	Molecular Biology of the Cell I, German Cancer Research Center
Genetic information	Exon3 of the glucocorticoid receptor is flanked by loxP sites allowing Cre-dependent excision.
Phenotypic information	None.
Breeding history	Backcrossed in C57BL/6 for >10 generations.
References	Disruption of the glucocorticoid receptor gene in the nervous system results in reduced anxiety.;Tronche F, Kellendonk C, Kretz O, Gass P, Anlag K, Orban P C, Bock R, Klein R, Schütz G, ;1999;Nature genetics;23;99-103; 10471508

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Generalized glucocorticoid resistance syndrome / Orphanet_786

MGI phenotypes (gene matching)

abnormal liver physiology / MGI
abnormal adrenal gland morphology / MGI
enlarged adrenal glands / MGI
absent adrenal medulla / MGI
absent adrenergic chromaffin cells / MGI
enlarged adrenocortical cells / MGI
abnormal lung morphology / MGI
abnormal lung development / MGI
atelectasis / MGI
thick pulmonary interalveolar septum / MGI
cyanosis / MGI
absent circulating adrenaline / MGI
increased circulating corticosterone level / MGI
increased circulating adrenocorticotropin level / MGI
respiratory failure / MGI
respiratory distress / MGI
no abnormal phenotype detected / MGI
abnormal pulmonary alveolus morphology / MGI
abnormal pulmonary alveolus epithelial cell morphology / MGI
abnormal alveolocapillary membrane morphology / MGI
decreased circulating adrenocorticotropin level / MGI
decreased circulating corticosterone level / MGI
increased circulating aldosterone level / MGI
small adrenal glands / MGI
decreased circulating testosterone level / MGI
abnormal respiratory system development / MGI
decreased macrophage cell number / MGI
pulmonary hyperplasia / MGI
abnormal surfactant secretion / MGI
increased susceptibility to experimental autoimmune encephalomyelitis / MGI
increased mean systemic arterial blood pressure / MGI
abnormal osteoblast physiology / MGI
decreased B cell number / MGI
abnormal blood-brain barrier function / MGI
abnormal circulating corticosterone level / MGI
renal/urinary system phenotype / MGI
adipose tissue phenotype / MGI
homeostasis/metabolism phenotype / MGI
growth/size/body region phenotype / MGI
skeleton phenotype / MGI
increased renin activity / MGI
increased lung weight / MGI
decreased circulating noradrenaline level / MGI
impaired lung alveolus development / MGI
increased systemic arterial systolic blood pressure / MGI
increased circulating angiotensinogen level / MGI
decreased T cell apoptosis / MGI
decreased CD4-positive, alpha beta T cell number / MGI
decreased CD8-positive, alpha-beta T cell number / MGI
abnormal adrenal cortex morphology / MGI
enlarged adrenocortical cell nuclei / MGI
abnormal adrenal gland zona glomerulosa morphology / MGI
abnormal adrenal gland zona fasciculata morphology / MGI
abnormal adrenal gland zona reticularis morphology / MGI
thin adrenal cortex / MGI
abnormal osteoblast differentiation / MGI
abnormal adrenal chromaffin cell morphology / MGI
decreased survivor rate / MGI
abnormal physiological response to xenobiotic / MGI
decreased erythroid progenitor cell number / MGI
abnormal thymocyte apoptosis / MGI
increased liver triglyceride level / MGI
increased type II pneumocyte number / MGI
decreased type I pneumocyte number / MGI
abnormal branching involved in terminal bronchiole morphogenesis / MGI
postnatal lethality, complete penetrance / MGI
neonatal lethality, complete penetrance / MGI
neonatal lethality, incomplete penetrance / MGI
perinatal lethality, complete penetrance / MGI
perinatal lethality, incomplete penetrance / MGI
decreased noradrenaline level / MGI
decreased adrenaline level / MGI

Literature references

Disruption of the glucocorticoid receptor gene in the nervous system results in reduced anxiety.;Tronche F, Kellendonk C, Kretz O, Gass P, Anlag K, Orban P C, Bock R, Klein R, Schütz G, ;1999;Nature genetics;23;99-103; 10471508
Circadian variation in pulmonary inflammatory responses is independent of rhythmic glucocorticoid signaling in airway epithelial cells.;Ince Louise M, Zhang Zhenguang, Beesley Stephen, Vonslow Ryan M, Saer Ben R, Matthews Laura C, Begley Nicola, Gibbs Julie E, Ray David W, Loudon Andrew S I, ;2019;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;33;126-139; 29965797
Glucocorticoid receptor is required for foetal heart maturation.;Rog-Zielinska Eva A, Thomson Adrian, Kenyon Christopher J, Brownstein David G, Moran Carmel M, Szumska Dorota, Michailidou Zoi, Richardson Jennifer, Owen Elizabeth, Watt Alistair, Morrison Harris, Forrester Lesley M, Bhattacharya Shoumo, Holmes Megan C, Chapman Karen E, ;2013;Human molecular genetics;22;3269-82; 23595884
Antenatal dexamethasone treatment transiently alters diastolic function in the mouse fetal heart.;Agnew E J, Garcia-Burgos A, Richardson R V, Manos H, Thomson A J W, Sooy K, Just G, Homer N Z M, Moran C M, Brunton P J, Gray G A, Chapman K E, ;2019;The Journal of endocrinology;241;279-292; 31013474
Glucocorticoid receptor alters isovolumetric contraction and restrains cardiac fibrosis.;Richardson Rachel V, Batchen Emma J, Thomson Adrian J W, Darroch Rowan, Pan Xinlu, Rog-Zielinska Eva A, Wyrzykowska Wiktoria, Scullion Kathleen, Al-Dujaili Emad A S, Diaz Mary E, Moran Carmel M, Kenyon Christopher J, Gray Gillian A, Chapman Karen E, ;2017;The Journal of endocrinology;232;437-450; 28057868
Glucocorticoids promote structural and functional maturation of foetal cardiomyocytes: a role for PGC-1α.;Rog-Zielinska E A, Craig M-A, Manning J R, Richardson R V, Gowans G J, Dunbar D R, Gharbi K, Kenyon C J, Holmes M C, Hardie D G, Smith G L, Chapman K E, ;2015;Cell death and differentiation;22;1106-16; 25361084
Hypothalamic AgRP neurons exert top-down control on systemic TNF-α release during endotoxemia.;Boutagouga Boudjadja Mehdi, Culotta Isabella, De Paula Gabriela C, Harno Erika, Hunter Jenna, Cavalcanti-de-Albuquerque João Paulo, Luckman Simon M, Hepworth Matthew, White Anne, Aviello Gabriella, D'Agostino Giuseppe, ;2022;Current biology : CB;32;4699-4706.e4; 36182699

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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B6.129P2-Nr3c1tm2Gsc/Ieg