B6Rcc.Cg-Spp1^tm1Rit Apc^Min/Cnrm

Status	Available to order
EMMA ID	EM:02002
International strain name	B6Rcc.Cg-Spp1^tm1Rit Apc^Min/Cnrm
Alternative name	C57 BL/6RCC Min/+ OPN-/-
Strain type	Induced Mutant Strains : Chemically-induced
Allele/Transgene symbol	Apc^Min, Spp1^tm1Rit
Gene/Transgene symbol	Apc, Spp1

Information from provider

Provider	Ursula Günthert
Provider affiliation	Institut für Pathologie, University of Basel
Genetic information	Ethylnitrosurea induced mutation. A transversion point mutation in the Apc (adenomatosis polyposis coli) gene that alters nucleotide 2549 from a T to an A and converts codon 850 from one encoding a leucine to a stop codon, generating the mutant allele Apc^Min (adenomatosis polyposis coli; multiple intestinal neoplasia). The neomycin cassette from pMC1neo was inserted into the EagI site in exon 6 of the Spp1 (secreted phosphoprotein 1; formerly: Opn, osteopontin) gene (a 4.8 kb fragment from 129 mice), that had been cloned into pBluescript (Rittling et al., 1998).
Phenotypic information	Min/+: development of early stages of colon carcinoma: polyps. Opn (Spp1) -/-: influence on colon carcinoma not known.
References	A dominant mutation that predisposes to multiple intestinal neoplasia in the mouse.;Moser A R, Pitot H C, Dove W F, ;1990;Science (New York, N.Y.);247;322-4; 2296722 Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitro.;Rittling S R, Matsumoto H N, McKee M D, Nanci A, An X R, Novick K E, Kowalski A J, Noda M, Denhardt D T, ;1998;Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research;13;1101-11; 9661074

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Desmoid tumor / Orphanet_873
Cenani-Lenz syndrome / Orphanet_3258
Turcot syndrome with polyposis / Orphanet_99818
Gastric adenocarcinoma and proximal polyposis of the stomach / Orphanet_314022
Gardner syndrome / Orphanet_79665
APC-related attenuated familial adenomatous polyposis / Orphanet_247806

IMPC phenotypes (gene matching)

anophthalmia / IMPC

MGI phenotypes (allele matching)

abnormal mammary gland development / MGI
abnormal ovarian folliculogenesis / MGI
abnormal ovarian follicle morphology / MGI
absent mature ovarian follicles / MGI
absent corpus luteum / MGI
abnormal vagina epithelium morphology / MGI
decreased body weight / MGI
increased incidence of induced tumors / MGI
ovary atrophy / MGI
uterus atrophy / MGI
reproductive system phenotype / MGI
adrenal gland hyperplasia / MGI
absent nipple / MGI
increased mammary gland tumor incidence / MGI
mammary gland hyperplasia / MGI
anemia / MGI
premature death / MGI
increased intestinal adenoma incidence / MGI
increased intestinal adenocarcinoma incidence / MGI
abnormal pregnancy / MGI
extended life span / MGI
aneuploidy / MGI
increased incidence of tumors by chemical induction / MGI
decreased intestinal adenoma incidence / MGI
decreased hematocrit / MGI
hyperlipidemia / MGI
melena / MGI
rectal prolapse / MGI
abnormal intestinal mucosa morphology / MGI
increased mammary adenocarcinoma incidence / MGI
intestinal obstruction / MGI
abnormal intestinal goblet cell morphology / MGI
abnormal large intestine crypts of Lieberkuhn morphology / MGI
intestine polyps / MGI
abnormal enterocyte proliferation / MGI
decreased T cell number / MGI
decreased NK cell number / MGI
decreased splenocyte number / MGI
neoplasm / MGI
increased circulating triglyceride level / MGI
increased circulating free fatty acid level / MGI
postnatal growth retardation / MGI
increased colonic adenoma incidence / MGI
hepatic steatosis / MGI
decreased macrophage cell number / MGI
increased prostaglandin level / MGI
abnormal embryo development / MGI
abnormal embryo size / MGI
abnormal egg cylinder morphology / MGI
abnormal cell physiology / MGI
embryonic lethality, complete penetrance / MGI
decreased embryo size / MGI
embryonic lethality between implantation and placentation / MGI
absent mandible / MGI
abnormal brain development / MGI
acrania / MGI
perinatal lethality, complete penetrance / MGI
lethality throughout fetal growth and development, incomplete penetrance / MGI
absent midbrain / MGI
absent forebrain / MGI
abnormal liver morphology / MGI
decreased acute inflammation / MGI
decreased tumor necrosis factor secretion / MGI
decreased interferon-gamma secretion / MGI
decreased physiological sensitivity to xenobiotic / MGI
increased bone mineral density / MGI
abnormal osteoclast physiology / MGI
abnormal cardiovascular system physiology / MGI
abnormal inflammatory response / MGI
decreased susceptibility to viral infection / MGI
dilated renal tubules / MGI
decreased heart weight / MGI
delayed wound healing / MGI
nervous system phenotype / MGI
abnormal physiological neovascularization / MGI
renal tubular necrosis / MGI
increased osteoclast cell number / MGI
decreased osteoclast cell number / MGI
decreased bone resorption / MGI
abnormal osteoblast physiology / MGI
abnormal response to infection / MGI
decreased cardiac muscle contractility / MGI
increased susceptibility to injury / MGI
skeleton phenotype / MGI
hyporesponsive to tactile stimuli / MGI
increased circulating creatinine level / MGI
increased blood urea nitrogen level / MGI
decreased susceptibility to type IV hypersensitivity reaction / MGI
increased interleukin-10 secretion / MGI
decreased interleukin-12 secretion / MGI
increased interleukin-4 secretion / MGI
decreased sensitivity to induced cell death / MGI
increased trabecular bone thickness / MGI
mortality/aging / MGI
decreased fibroblast chemotaxis / MGI
increased bone marrow cell number / MGI
abnormal blood cell morphology/development / MGI
abnormal bone mineralization / MGI
increased hematopoietic stem cell number / MGI
increased sensitivity to induced morbidity/mortality / MGI

MGI phenotypes (gene matching)

absent mandible / MGI
decreased hematocrit / MGI
increased cell proliferation / MGI
abnormal digestive system morphology / MGI
gastrointestinal hemorrhage / MGI
abnormal foregut morphology / MGI
abnormal crypts of Lieberkuhn morphology / MGI
rectal prolapse / MGI
abnormal intestinal mucosa morphology / MGI
abnormal mammary gland development / MGI
mammary gland hyperplasia / MGI
abnormal brain development / MGI
abnormal floor plate morphology / MGI
abnormal ovarian folliculogenesis / MGI
abnormal ovarian follicle morphology / MGI
absent mature ovarian follicles / MGI
absent corpus luteum / MGI
abnormal vagina epithelium morphology / MGI
dermal cysts / MGI
decreased body weight / MGI
decreased body size / MGI
increased metastatic potential / MGI
abnormal nursing / MGI
hunched posture / MGI
hyperlipidemia / MGI
increased circulating triglyceride level / MGI
increased circulating free fatty acid level / MGI
anemia / MGI
extended life span / MGI
abnormal embryo development / MGI
abnormal embryo size / MGI
decreased embryo size / MGI
incomplete embryo turning / MGI
postnatal growth retardation / MGI
increased mammary adenocarcinoma incidence / MGI
neoplasm / MGI
increased pilomatricoma incidence / MGI
increased tumor incidence / MGI
increased incidence of induced tumors / MGI
increased colon adenoma incidence / MGI
premature death / MGI
abnormal developmental patterning / MGI
abnormal embryonic tissue morphology / MGI
abnormal neural tube morphology / MGI
no abnormal phenotype detected / MGI
increased intestinal adenoma incidence / MGI
hepatic steatosis / MGI
increased trichoepithelioma incidence / MGI
increased intestinal adenocarcinoma incidence / MGI
no phenotypic analysis / MGI
abnormal egg cylinder morphology / MGI
acrania / MGI
colon polyps / MGI
intestinal obstruction / MGI
melena / MGI
rectal hemorrhage / MGI
gastric polyps / MGI
increased hepatocellular carcinoma incidence / MGI
epidermal cyst / MGI
decreased tumor growth/size / MGI
abnormal tumor morphology / MGI
abnormal intestinal goblet cell morphology / MGI
increased tumor growth/size / MGI
increased osteosarcoma incidence / MGI
decreased macrophage cell number / MGI
abnormal embryonic epiblast morphology / MGI
aneuploidy / MGI
abnormal primitive node morphology / MGI
cardia bifida / MGI
increased incidence of tumors by chemical induction / MGI
split notochord / MGI
ovary atrophy / MGI
abnormal large intestine crypts of Lieberkuhn morphology / MGI
uterus atrophy / MGI
decreased T cell number / MGI
increased circulating cholesterol level / MGI
cellular phenotype / MGI
reproductive system phenotype / MGI
abnormal cell physiology / MGI
increased apoptosis / MGI
abnormal hindbrain development / MGI
absent male preputial gland / MGI
intestine polyps / MGI
increased liver tumor incidence / MGI
decreased NK cell number / MGI
abnormal anterior visceral endoderm morphology / MGI
embryonic lethality / MGI
abnormal enterocyte proliferation / MGI
adrenal gland hyperplasia / MGI
abnormal mesendoderm development / MGI
increased small intestine adenocarcinoma incidence / MGI
increased large intestine adenocarcinoma incidence / MGI
decreased splenocyte number / MGI
abnormal pregnancy / MGI
absent nipple / MGI
increased prostaglandin level / MGI
embryonic lethality between implantation and placentation / MGI
increased gastrointestinal tumor incidence / MGI
increased mammary gland tumor incidence / MGI
increased skin tumor incidence / MGI
increased stomach tumor incidence / MGI
increased osteoma incidence / MGI
increased desmoid tumor incidence / MGI
gastrointestinal tract polyps / MGI
increased adenoma incidence / MGI
altered tumor pathology / MGI
mortality/aging / MGI
postnatal lethality, complete penetrance / MGI
postnatal lethality, incomplete penetrance / MGI
perinatal lethality, complete penetrance / MGI
prenatal lethality, complete penetrance / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
embryonic lethality between implantation and somite formation, incomplete penetrance / MGI
lethality throughout fetal growth and development, incomplete penetrance / MGI
absent midbrain / MGI
embryonic-extraembryonic boundary constriction / MGI
absent forebrain / MGI
rostral-caudal axis duplication / MGI
rostral body truncation / MGI
absent neural folds / MGI
abnormal head development / MGI
decreased intestinal adenoma incidence / MGI
abnormal endocardial heart tube morphology / MGI
increased bone mineral density / MGI
abnormal long bone metaphysis morphology / MGI
abnormal microglial cell morphology / MGI
decreased hematocrit / MGI
decreased leukocyte cell number / MGI
abnormal blood flow velocity / MGI
abnormal blood vessel physiology / MGI
increased bone marrow cell number / MGI
altered response to myocardial infarction / MGI
abnormal liver morphology / MGI
abnormal substantia nigra morphology / MGI
abnormal osteoclast physiology / MGI
abnormal cardiovascular system physiology / MGI
abnormal hematopoietic system physiology / MGI
abnormal vasodilation / MGI
abnormal blood vessel morphology / MGI
abnormal immune system physiology / MGI
altered susceptibility to infection / MGI
abnormal inflammatory response / MGI
decreased susceptibility to viral infection / MGI
decreased susceptibility to bacterial infection / MGI
increased susceptibility to bacterial infection / MGI
abnormal blood cell morphology/development / MGI
abnormal leukocyte physiology / MGI
abnormal macrophage morphology / MGI
abnormal macrophage physiology / MGI
granulomatous inflammation / MGI
increased heart rate / MGI
decreased circulating triglyceride level / MGI
dilated renal tubules / MGI
decreased circulating insulin level / MGI
decreased heart weight / MGI
decreased systemic arterial blood pressure / MGI
decreased hemoglobin content / MGI
increased insulin sensitivity / MGI
abnormal bone mineralization / MGI
delayed wound healing / MGI
abnormal leukocyte migration / MGI
nephrocalcinosis / MGI
nervous system phenotype / MGI
abnormal physiological neovascularization / MGI
impaired macrophage chemotaxis / MGI
decreased macrophage cell number / MGI
decreased lean body mass / MGI
abnormal striatum morphology / MGI
renal tubular necrosis / MGI
decreased incidence of tumors by chemical induction / MGI
increased hematopoietic stem cell number / MGI
decreased susceptibility to experimental autoimmune uveoretinitis / MGI
lung cysts / MGI
abnormal vascular wound healing / MGI
increased osteoclast cell number / MGI
decreased osteoclast cell number / MGI
decreased bone resorption / MGI
abnormal osteoblast physiology / MGI
increased lymphocyte cell number / MGI
abnormal wound healing / MGI
abnormal response to infection / MGI
decreased acute inflammation / MGI
decreased cardiac muscle contractility / MGI
increased susceptibility to injury / MGI
improved glucose tolerance / MGI
decreased susceptibility to atherosclerosis / MGI
renal/urinary system phenotype / MGI
homeostasis/metabolism phenotype / MGI
reproductive system phenotype / MGI
skeleton phenotype / MGI
increased macrophage cell number / MGI
hyporesponsive to tactile stimuli / MGI
increased circulating creatinine level / MGI
decreased circulating glucose level / MGI
increased blood urea nitrogen level / MGI
decreased susceptibility to type IV hypersensitivity reaction / MGI
increased lung weight / MGI
calcified aortic valve / MGI
decreased systemic arterial systolic blood pressure / MGI
decreased urine sodium level / MGI
decreased dendritic cell number / MGI
decreased tumor necrosis factor secretion / MGI
decreased interferon-gamma secretion / MGI
decreased circulating interleukin-6 level / MGI
increased interleukin-10 secretion / MGI
decreased interleukin-12 secretion / MGI
increased interleukin-4 secretion / MGI
abnormal chemokine level / MGI
increased physiological sensitivity to xenobiotic / MGI
decreased physiological sensitivity to xenobiotic / MGI
decreased sensitivity to induced cell death / MGI
increased neuron number / MGI
increased trabecular bone thickness / MGI
crystalluria / MGI
increased sensitivity to induced morbidity/mortality / MGI
decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
increased respiratory quotient / MGI
abnormal macrophage chemotaxis / MGI
mortality/aging / MGI
decreased susceptibility to dopaminergic neuron neurotoxicity / MGI
decreased urine chloride ion level / MGI
decreased fibroblast chemotaxis / MGI

Literature references

A dominant mutation that predisposes to multiple intestinal neoplasia in the mouse.;Moser A R, Pitot H C, Dove W F, ;1990;Science (New York, N.Y.);247;322-4; 2296722
Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitro.;Rittling S R, Matsumoto H N, McKee M D, Nanci A, An X R, Novick K E, Kowalski A J, Noda M, Denhardt D T, ;1998;Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research;13;1101-11; 9661074

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6Rcc.Cg-Spp1tm1Rit ApcMin/Cnrm