B6Rcc.Cg-Cd44^tm1.1Ugu Apc^Min/Cnrm

Status	Available to order
EMMA ID	EM:02001
International strain name	B6Rcc.Cg-Cd44^tm1.1Ugu Apc^Min/Cnrm
Alternative name	C57 BL/6RCC Min/+ CD44v6v7-/-
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Cd44^tm1.1Ugu, Apc^Min
Gene/Transgene symbol	Cd44, Apc

Information from provider

Provider	Ursula Günthert
Provider affiliation	Institut für Pathologie, University of Basel
Genetic information	Ethylnitrosurea induced mutation. A transversion point mutation in the Apc (adenomatosis polyposis coli) gene that alters nucleotide 2549 from a T to an A and converts codon 850 from one encoding a leucine to a stop codon, generating the mutant allele Apc^Min(adenomatosis polyposis coli; multiple intestinal neoplasia). A loxP site was inserted 3' of exon v6 and a neomycin resistance cassette, with a 5' loxP site, was inserted 5' of exon v6 of the Cd44 gene. During insertion, 1.6 kb of sequence was lost in the exon v7 region; cre recombinase was transiently expressed in ES cells allowing the removal of neomycin cassette and exon v6. Mice lack both exon v6 and v7.
Phenotypic information	Min/+: Development of early stages of colon carcinoma: polyps. Cd44v6v7-/-: Expression of Cd44v6 is an independent prognostic factor for a poor prognosis in colon carcinoma. Expression of Cd44v6 isoforms has been shown to be an independent prognostic factor for a poor prognosis in human colon carcinoma (Mulder et al., 1994). To determine whether and to what extent Cd44v6/v7-containing isoforms are involved in the early stages of adenoma formation, we crossbred Apc^Min mice with mice bearing deletions for Cd44v6/v7. While Min/+ x Cd44v6/v7 wild-type mice developed on the average more than 50 adenomas at an age of 12-18 weeks, Min/+ x Cd44v6/v7 knock-out mice developed fewer than 10 adenomas, and many had no polyps at all. Moreover, the life span of Min/+ x Cd44v6/v7 null mice increased substantially to more than 60 weeks (p
Breeding history	10 generations backcrossed onto C57BL/6Rcc; currently maintained as C57BL/6Rcc Min/+ Cd44v6v7-/-.
References	Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330 A dominant mutation that predisposes to multiple intestinal neoplasia in the mouse.;Moser A R, Pitot H C, Dove W F, ;1990;Science (New York, N.Y.);247;322-4; 2296722

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Desmoid tumor / Orphanet_873
Cenani-Lenz syndrome / Orphanet_3258
Turcot syndrome with polyposis / Orphanet_99818
Gastric adenocarcinoma and proximal polyposis of the stomach / Orphanet_314022
Gardner syndrome / Orphanet_79665
APC-related attenuated familial adenomatous polyposis / Orphanet_247806

MGI phenotypes (allele matching)

abnormal mammary gland development / MGI
abnormal ovarian folliculogenesis / MGI
abnormal ovarian follicle morphology / MGI
absent mature ovarian follicles / MGI
absent corpus luteum / MGI
abnormal vagina epithelium morphology / MGI
decreased body weight / MGI
increased incidence of induced tumors / MGI
ovary atrophy / MGI
uterus atrophy / MGI
reproductive system phenotype / MGI
adrenal gland hyperplasia / MGI
absent nipple / MGI
increased mammary gland tumor incidence / MGI
mammary gland hyperplasia / MGI
anemia / MGI
premature death / MGI
increased intestinal adenoma incidence / MGI
increased intestinal adenocarcinoma incidence / MGI
abnormal pregnancy / MGI
extended life span / MGI
aneuploidy / MGI
increased incidence of tumors by chemical induction / MGI
decreased intestinal adenoma incidence / MGI
decreased hematocrit / MGI
hyperlipidemia / MGI
melena / MGI
rectal prolapse / MGI
abnormal intestinal mucosa morphology / MGI
increased mammary adenocarcinoma incidence / MGI
intestinal obstruction / MGI
abnormal intestinal goblet cell morphology / MGI
abnormal large intestine crypts of Lieberkuhn morphology / MGI
intestine polyps / MGI
abnormal enterocyte proliferation / MGI
decreased T cell number / MGI
decreased NK cell number / MGI
decreased splenocyte number / MGI
neoplasm / MGI
increased circulating triglyceride level / MGI
increased circulating free fatty acid level / MGI
postnatal growth retardation / MGI
increased colonic adenoma incidence / MGI
hepatic steatosis / MGI
decreased macrophage cell number / MGI
increased prostaglandin level / MGI
abnormal embryo development / MGI
abnormal embryo size / MGI
abnormal egg cylinder morphology / MGI
abnormal cell physiology / MGI
embryonic lethality, complete penetrance / MGI
decreased embryo size / MGI
embryonic lethality between implantation and placentation / MGI
absent mandible / MGI
abnormal brain development / MGI
acrania / MGI
perinatal lethality, complete penetrance / MGI
lethality throughout fetal growth and development, incomplete penetrance / MGI
absent midbrain / MGI
absent forebrain / MGI
decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
decreased T cell proliferation / MGI
abnormal digestive system physiology / MGI
immune system phenotype / MGI
abnormal T-helper 1 physiology / MGI
abnormal T-helper 2 physiology / MGI

MGI phenotypes (gene matching)

absent mandible / MGI
decreased hematocrit / MGI
increased cell proliferation / MGI
abnormal digestive system morphology / MGI
gastrointestinal hemorrhage / MGI
abnormal foregut morphology / MGI
abnormal crypts of Lieberkuhn morphology / MGI
rectal prolapse / MGI
abnormal intestinal mucosa morphology / MGI
abnormal mammary gland development / MGI
mammary gland hyperplasia / MGI
abnormal brain development / MGI
abnormal floor plate morphology / MGI
abnormal ovarian folliculogenesis / MGI
abnormal ovarian follicle morphology / MGI
absent mature ovarian follicles / MGI
absent corpus luteum / MGI
abnormal vagina epithelium morphology / MGI
dermal cysts / MGI
decreased body weight / MGI
decreased body size / MGI
increased metastatic potential / MGI
abnormal nursing / MGI
hunched posture / MGI
hyperlipidemia / MGI
increased circulating triglyceride level / MGI
increased circulating free fatty acid level / MGI
anemia / MGI
extended life span / MGI
abnormal embryo development / MGI
abnormal embryo size / MGI
decreased embryo size / MGI
incomplete embryo turning / MGI
postnatal growth retardation / MGI
increased mammary adenocarcinoma incidence / MGI
neoplasm / MGI
increased pilomatricoma incidence / MGI
increased tumor incidence / MGI
increased incidence of induced tumors / MGI
increased colon adenoma incidence / MGI
premature death / MGI
abnormal developmental patterning / MGI
abnormal embryonic tissue morphology / MGI
abnormal neural tube morphology / MGI
no abnormal phenotype detected / MGI
increased intestinal adenoma incidence / MGI
hepatic steatosis / MGI
increased trichoepithelioma incidence / MGI
increased intestinal adenocarcinoma incidence / MGI
no phenotypic analysis / MGI
abnormal egg cylinder morphology / MGI
acrania / MGI
colon polyps / MGI
intestinal obstruction / MGI
melena / MGI
rectal hemorrhage / MGI
gastric polyps / MGI
increased hepatocellular carcinoma incidence / MGI
epidermal cyst / MGI
decreased tumor growth/size / MGI
abnormal tumor morphology / MGI
abnormal intestinal goblet cell morphology / MGI
increased tumor growth/size / MGI
increased osteosarcoma incidence / MGI
decreased macrophage cell number / MGI
abnormal embryonic epiblast morphology / MGI
aneuploidy / MGI
abnormal primitive node morphology / MGI
cardia bifida / MGI
increased incidence of tumors by chemical induction / MGI
split notochord / MGI
ovary atrophy / MGI
abnormal large intestine crypts of Lieberkuhn morphology / MGI
uterus atrophy / MGI
decreased T cell number / MGI
increased circulating cholesterol level / MGI
cellular phenotype / MGI
reproductive system phenotype / MGI
abnormal cell physiology / MGI
increased apoptosis / MGI
abnormal hindbrain development / MGI
absent male preputial gland / MGI
intestine polyps / MGI
increased liver tumor incidence / MGI
decreased NK cell number / MGI
abnormal anterior visceral endoderm morphology / MGI
embryonic lethality / MGI
abnormal enterocyte proliferation / MGI
adrenal gland hyperplasia / MGI
abnormal mesendoderm development / MGI
increased small intestine adenocarcinoma incidence / MGI
increased large intestine adenocarcinoma incidence / MGI
decreased splenocyte number / MGI
abnormal pregnancy / MGI
absent nipple / MGI
increased prostaglandin level / MGI
embryonic lethality between implantation and placentation / MGI
increased gastrointestinal tumor incidence / MGI
increased mammary gland tumor incidence / MGI
increased skin tumor incidence / MGI
increased stomach tumor incidence / MGI
increased osteoma incidence / MGI
increased desmoid tumor incidence / MGI
gastrointestinal tract polyps / MGI
increased adenoma incidence / MGI
altered tumor pathology / MGI
mortality/aging / MGI
postnatal lethality, complete penetrance / MGI
postnatal lethality, incomplete penetrance / MGI
perinatal lethality, complete penetrance / MGI
prenatal lethality, complete penetrance / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
embryonic lethality between implantation and somite formation, incomplete penetrance / MGI
lethality throughout fetal growth and development, incomplete penetrance / MGI
absent midbrain / MGI
embryonic-extraembryonic boundary constriction / MGI
absent forebrain / MGI
rostral-caudal axis duplication / MGI
rostral body truncation / MGI
absent neural folds / MGI
abnormal head development / MGI
decreased intestinal adenoma incidence / MGI
abnormal endocardial heart tube morphology / MGI
decreased mast cell number / MGI
altered response to myocardial infarction / MGI
abnormal cell morphology / MGI
decreased mast cell histamine storage / MGI
abnormal uterus morphology / MGI
thin epidermis / MGI
abnormal lipid level / MGI
abnormal vasculogenesis / MGI
abnormal digestive system physiology / MGI
impaired wound healing / MGI
impaired macrophage phagocytosis / MGI
liver inflammation / MGI
lung inflammation / MGI
abnormal definitive hematopoiesis / MGI
no abnormal phenotype detected / MGI
abnormal CD4-positive, alpha beta T cell morphology / MGI
abnormal neutrophil physiology / MGI
short tibia / MGI
impaired skin barrier function / MGI
delayed wound healing / MGI
decreased myocardial infarction size / MGI
abnormal leukocyte migration / MGI
peritoneal inflammation / MGI
decreased susceptibility to induced arthritis / MGI
decreased tumor growth/size / MGI
abnormal leukocyte adhesion / MGI
abnormal physiological neovascularization / MGI
increased susceptibility to induced arthritis / MGI
enhanced coordination / MGI
decreased cholesterol level / MGI
abnormal vascular endothelial cell physiology / MGI
decreased susceptibility to kidney reperfusion injury / MGI
abnormal miniature excitatory postsynaptic currents / MGI
decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
increased mean systemic arterial blood pressure / MGI
abnormal CD8-positive, alpha beta T cell morphology / MGI
abnormal response to infection / MGI
increased susceptibility to parasitic infection / MGI
impaired natural killer cell mediated cytotoxicity / MGI
decreased acute inflammation / MGI
increased acute inflammation / MGI
decreased T cell proliferation / MGI
digestive/alimentary phenotype / MGI
immune system phenotype / MGI
hematopoietic system phenotype / MGI
abnormal CD4-positive, alpha-beta T cell physiology / MGI
abnormal T-helper 1 physiology / MGI
abnormal T-helper 2 physiology / MGI
choroidal neovascularization / MGI
decreased circulating creatinine level / MGI
decreased blood urea nitrogen level / MGI
abnormal vascular smooth muscle physiology / MGI
decreased angiogenesis / MGI
decreased susceptibility to type IV hypersensitivity reaction / MGI
abnormal cell physiology / MGI
abnormal vascular endothelial cell morphology / MGI
abnormal involution of the mammary gland / MGI
increased diameter of tibia / MGI
abnormal osteoclast differentiation / MGI
increased circulating tumor necrosis factor level / MGI
decreased circulating tumor necrosis factor level / MGI
increased tumor necrosis factor secretion / MGI
abnormal interleukin secretion / MGI
increased circulating interferon-gamma level / MGI
increased circulating interleukin-2 level / MGI
increased interleukin-1 beta secretion / MGI
increased interleukin-10 secretion / MGI
increased interleukin-6 secretion / MGI
impaired neutrophil recruitment / MGI
abnormal chemokine secretion / MGI
decreased transforming growth factor level / MGI
decreased sensitivity to induced cell death / MGI
abnormal epidermal lamellar body morphology / MGI
increased sensitivity to induced morbidity/mortality / MGI
abnormal circulating chemokine level / MGI
abnormal circulating cytokine level / MGI
decreased activation-induced cell death of T cells / MGI
increased mitochondria size / MGI
abnormal mitochondrial crista morphology / MGI
decreased fibroblast proliferation / MGI
impaired leukocyte tethering or rolling / MGI

Literature references

Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330
A dominant mutation that predisposes to multiple intestinal neoplasia in the mouse.;Moser A R, Pitot H C, Dove W F, ;1990;Science (New York, N.Y.);247;322-4; 2296722

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Example health report
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Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6Rcc.Cg-Cd44tm1.1Ugu ApcMin/Cnrm