B6Rcc.Cg-Cd44^tm1.1Ugu Apc^Min/Cnrm

Status	Available to order
EMMA ID	EM:02001
Citation information	RRID:IMSR_EM:02001 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6Rcc.Cg-Cd44^tm1.1Ugu Apc^Min/Cnrm
Alternative name	C57 BL/6RCC Min/+ CD44v6v7-/-
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Cd44^tm1.1Ugu, Apc^Min
Gene/Transgene symbol	Cd44, Apc

Information from provider

Provider	Ursula Günthert
Provider affiliation	Institut für Pathologie, University of Basel
Genetic information	Ethylnitrosurea induced mutation. A transversion point mutation in the Apc (adenomatosis polyposis coli) gene that alters nucleotide 2549 from a T to an A and converts codon 850 from one encoding a leucine to a stop codon, generating the mutant allele Apc^Min(adenomatosis polyposis coli; multiple intestinal neoplasia). A loxP site was inserted 3' of exon v6 and a neomycin resistance cassette, with a 5' loxP site, was inserted 5' of exon v6 of the Cd44 gene. During insertion, 1.6 kb of sequence was lost in the exon v7 region; cre recombinase was transiently expressed in ES cells allowing the removal of neomycin cassette and exon v6. Mice lack both exon v6 and v7.
Phenotypic information	Min/+: Development of early stages of colon carcinoma: polyps. Cd44v6v7-/-: Expression of Cd44v6 is an independent prognostic factor for a poor prognosis in colon carcinoma. Expression of Cd44v6 isoforms has been shown to be an independent prognostic factor for a poor prognosis in human colon carcinoma (Mulder et al., 1994). To determine whether and to what extent Cd44v6/v7-containing isoforms are involved in the early stages of adenoma formation, we crossbred Apc^Min mice with mice bearing deletions for Cd44v6/v7. While Min/+ x Cd44v6/v7 wild-type mice developed on the average more than 50 adenomas at an age of 12-18 weeks, Min/+ x Cd44v6/v7 knock-out mice developed fewer than 10 adenomas, and many had no polyps at all. Moreover, the life span of Min/+ x Cd44v6/v7 null mice increased substantially to more than 60 weeks (p
Breeding history	10 generations backcrossed onto C57BL/6Rcc; currently maintained as C57BL/6Rcc Min/+ Cd44v6v7-/-.
References	Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330 A dominant mutation that predisposes to multiple intestinal neoplasia in the mouse.;Moser A R, Pitot H C, Dove W F, ;1990;Science (New York, N.Y.);247;322-4; 2296722

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Desmoid tumor / Orphanet_873
Cenani-Lenz syndrome / Orphanet_3258
Turcot syndrome with polyposis / Orphanet_99818
Gastric adenocarcinoma and proximal polyposis of the stomach / Orphanet_314022
Gardner syndrome / Orphanet_79665
APC-related attenuated familial adenomatous polyposis / Orphanet_247806

MGI phenotypes (allele matching)

abnormal mammary gland development / MGI
abnormal ovarian folliculogenesis / MGI
abnormal ovarian follicle morphology / MGI
absent mature ovarian follicles / MGI
absent corpus luteum / MGI
abnormal vagina epithelium morphology / MGI
decreased body weight / MGI
increased incidence of induced tumors / MGI
ovary atrophy / MGI
uterus atrophy / MGI
reproductive system phenotype / MGI
adrenal gland hyperplasia / MGI
absent nipple / MGI
increased mammary gland tumor incidence / MGI
mammary gland hyperplasia / MGI
anemia / MGI
premature death / MGI
increased intestinal adenoma incidence / MGI
increased intestinal adenocarcinoma incidence / MGI
abnormal pregnancy / MGI
extended life span / MGI
aneuploidy / MGI
increased incidence of tumors by chemical induction / MGI
decreased intestinal adenoma incidence / MGI
decreased hematocrit / MGI
hyperlipidemia / MGI
melena / MGI
rectal prolapse / MGI
abnormal intestinal mucosa morphology / MGI
increased mammary adenocarcinoma incidence / MGI
intestinal obstruction / MGI
abnormal intestinal goblet cell morphology / MGI
abnormal large intestine crypts of Lieberkuhn morphology / MGI
intestine polyps / MGI
abnormal enterocyte proliferation / MGI
decreased T cell number / MGI
decreased NK cell number / MGI
decreased splenocyte number / MGI
neoplasm / MGI
increased circulating triglyceride level / MGI
increased circulating free fatty acid level / MGI
postnatal growth retardation / MGI
increased colonic adenoma incidence / MGI
hepatic steatosis / MGI
decreased macrophage cell number / MGI
increased prostaglandin level / MGI
abnormal embryo development / MGI
abnormal embryo size / MGI
abnormal egg cylinder morphology / MGI
abnormal cell physiology / MGI
embryonic lethality, complete penetrance / MGI
decreased embryo size / MGI
embryonic lethality between implantation and placentation / MGI
absent mandible / MGI
abnormal brain development / MGI
acrania / MGI
perinatal lethality, complete penetrance / MGI
lethality throughout fetal growth and development, incomplete penetrance / MGI
absent midbrain / MGI
absent forebrain / MGI
decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
decreased T cell proliferation / MGI
abnormal digestive system physiology / MGI
immune system phenotype / MGI
abnormal T-helper 1 physiology / MGI
abnormal T-helper 2 physiology / MGI

MGI phenotypes (gene matching)

absent mandible / MGI
decreased hematocrit / MGI
increased cell proliferation / MGI
abnormal digestive system morphology / MGI
gastrointestinal hemorrhage / MGI
abnormal foregut morphology / MGI
abnormal crypts of Lieberkuhn morphology / MGI
rectal prolapse / MGI
abnormal intestinal mucosa morphology / MGI
abnormal mammary gland development / MGI
mammary gland hyperplasia / MGI
abnormal brain development / MGI
abnormal floor plate morphology / MGI
abnormal ovarian folliculogenesis / MGI
abnormal ovarian follicle morphology / MGI
absent mature ovarian follicles / MGI
absent corpus luteum / MGI
abnormal vagina epithelium morphology / MGI
dermal cysts / MGI
decreased body weight / MGI
decreased body size / MGI
increased metastatic potential / MGI
abnormal nursing / MGI
hunched posture / MGI
hyperlipidemia / MGI
increased circulating triglyceride level / MGI
increased circulating free fatty acid level / MGI
anemia / MGI
extended life span / MGI
abnormal embryo development / MGI
abnormal embryo size / MGI
decreased embryo size / MGI
incomplete embryo turning / MGI
postnatal growth retardation / MGI
increased mammary adenocarcinoma incidence / MGI
neoplasm / MGI
increased pilomatricoma incidence / MGI
increased tumor incidence / MGI
increased incidence of induced tumors / MGI
increased colon adenoma incidence / MGI
premature death / MGI
abnormal developmental patterning / MGI
abnormal embryonic tissue morphology / MGI
abnormal neural tube morphology / MGI
no abnormal phenotype detected / MGI
increased intestinal adenoma incidence / MGI
hepatic steatosis / MGI
increased trichoepithelioma incidence / MGI
increased intestinal adenocarcinoma incidence / MGI
no phenotypic analysis / MGI
abnormal egg cylinder morphology / MGI
acrania / MGI
colon polyps / MGI
intestinal obstruction / MGI
melena / MGI
rectal hemorrhage / MGI
gastric polyps / MGI
increased hepatocellular carcinoma incidence / MGI
epidermal cyst / MGI
decreased tumor growth/size / MGI
abnormal tumor morphology / MGI
abnormal intestinal goblet cell morphology / MGI
increased tumor growth/size / MGI
increased osteosarcoma incidence / MGI
decreased macrophage cell number / MGI
abnormal embryonic epiblast morphology / MGI
aneuploidy / MGI
abnormal primitive node morphology / MGI
cardia bifida / MGI
increased incidence of tumors by chemical induction / MGI
split notochord / MGI
ovary atrophy / MGI
abnormal large intestine crypts of Lieberkuhn morphology / MGI
uterus atrophy / MGI
decreased T cell number / MGI
increased circulating cholesterol level / MGI
cellular phenotype / MGI
reproductive system phenotype / MGI
abnormal cell physiology / MGI
increased apoptosis / MGI
abnormal hindbrain development / MGI
absent male preputial gland / MGI
intestine polyps / MGI
increased liver tumor incidence / MGI
decreased NK cell number / MGI
abnormal anterior visceral endoderm morphology / MGI
embryonic lethality / MGI
abnormal enterocyte proliferation / MGI
adrenal gland hyperplasia / MGI
abnormal mesendoderm development / MGI
increased small intestine adenocarcinoma incidence / MGI
increased large intestine adenocarcinoma incidence / MGI
decreased splenocyte number / MGI
abnormal pregnancy / MGI
absent nipple / MGI
increased prostaglandin level / MGI
embryonic lethality between implantation and placentation / MGI
increased gastrointestinal tumor incidence / MGI
increased mammary gland tumor incidence / MGI
increased skin tumor incidence / MGI
increased stomach tumor incidence / MGI
increased osteoma incidence / MGI
increased desmoid tumor incidence / MGI
gastrointestinal tract polyps / MGI
increased adenoma incidence / MGI
altered tumor pathology / MGI
mortality/aging / MGI
postnatal lethality, complete penetrance / MGI
postnatal lethality, incomplete penetrance / MGI
perinatal lethality, complete penetrance / MGI
prenatal lethality, complete penetrance / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
embryonic lethality between implantation and somite formation, incomplete penetrance / MGI
lethality throughout fetal growth and development, incomplete penetrance / MGI
absent midbrain / MGI
embryonic-extraembryonic boundary constriction / MGI
absent forebrain / MGI
rostral-caudal axis duplication / MGI
rostral body truncation / MGI
absent neural folds / MGI
abnormal head development / MGI
decreased intestinal adenoma incidence / MGI
abnormal endocardial heart tube morphology / MGI
decreased mast cell number / MGI
altered response to myocardial infarction / MGI
abnormal cell morphology / MGI
decreased mast cell histamine storage / MGI
abnormal uterus morphology / MGI
thin epidermis / MGI
abnormal lipid level / MGI
abnormal vasculogenesis / MGI
abnormal digestive system physiology / MGI
impaired wound healing / MGI
impaired macrophage phagocytosis / MGI
liver inflammation / MGI
lung inflammation / MGI
abnormal definitive hematopoiesis / MGI
no abnormal phenotype detected / MGI
abnormal CD4-positive, alpha beta T cell morphology / MGI
abnormal neutrophil physiology / MGI
short tibia / MGI
impaired skin barrier function / MGI
delayed wound healing / MGI
decreased myocardial infarction size / MGI
abnormal leukocyte migration / MGI
peritoneal inflammation / MGI
decreased susceptibility to induced arthritis / MGI
decreased tumor growth/size / MGI
abnormal leukocyte adhesion / MGI
abnormal physiological neovascularization / MGI
increased susceptibility to induced arthritis / MGI
enhanced coordination / MGI
decreased cholesterol level / MGI
abnormal vascular endothelial cell physiology / MGI
decreased susceptibility to kidney reperfusion injury / MGI
abnormal miniature excitatory postsynaptic currents / MGI
decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
increased mean systemic arterial blood pressure / MGI
abnormal CD8-positive, alpha beta T cell morphology / MGI
abnormal response to infection / MGI
increased susceptibility to parasitic infection / MGI
impaired natural killer cell mediated cytotoxicity / MGI
decreased acute inflammation / MGI
increased acute inflammation / MGI
decreased T cell proliferation / MGI
digestive/alimentary phenotype / MGI
immune system phenotype / MGI
hematopoietic system phenotype / MGI
abnormal CD4-positive, alpha-beta T cell physiology / MGI
abnormal T-helper 1 physiology / MGI
abnormal T-helper 2 physiology / MGI
choroidal neovascularization / MGI
decreased circulating creatinine level / MGI
decreased blood urea nitrogen level / MGI
abnormal vascular smooth muscle physiology / MGI
decreased angiogenesis / MGI
decreased susceptibility to type IV hypersensitivity reaction / MGI
abnormal cell physiology / MGI
abnormal vascular endothelial cell morphology / MGI
abnormal involution of the mammary gland / MGI
increased diameter of tibia / MGI
abnormal osteoclast differentiation / MGI
increased circulating tumor necrosis factor level / MGI
decreased circulating tumor necrosis factor level / MGI
increased tumor necrosis factor secretion / MGI
abnormal interleukin secretion / MGI
increased circulating interferon-gamma level / MGI
increased circulating interleukin-2 level / MGI
increased interleukin-1 beta secretion / MGI
increased interleukin-10 secretion / MGI
increased interleukin-6 secretion / MGI
impaired neutrophil recruitment / MGI
abnormal chemokine secretion / MGI
decreased transforming growth factor level / MGI
decreased sensitivity to induced cell death / MGI
abnormal epidermal lamellar body morphology / MGI
increased sensitivity to induced morbidity/mortality / MGI
abnormal circulating chemokine level / MGI
abnormal circulating cytokine level / MGI
decreased activation-induced cell death of T cells / MGI
increased mitochondria size / MGI
abnormal mitochondrial crista morphology / MGI
decreased fibroblast proliferation / MGI
impaired leukocyte tethering or rolling / MGI

Literature references

Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330
A dominant mutation that predisposes to multiple intestinal neoplasia in the mouse.;Moser A R, Pitot H C, Dove W F, ;1990;Science (New York, N.Y.);247;322-4; 2296722

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Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

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B6Rcc.Cg-Cd44tm1.1Ugu ApcMin/Cnrm