MGI phenotypes (allele matching)
B6Rcc.Cg-Ptprca Cd44tm2.1Ugu/Cnrm
| Status | Available to order |
| EMMA ID | EM:01997 |
| International strain name | B6Rcc.Cg-Ptprca Cd44tm2.1Ugu/Cnrm |
| Alternative name | C57 BL/6RCC CD44v10-/- Ly5.1 |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Cd44tm2.1Ugu, Ptprca |
| Gene/Transgene symbol | Cd44, Ptprc |
Information from provider
| Provider | Ursula Günthert |
| Provider affiliation | Institut für Pathologie, University of Basel |
| Genetic information | The mouse Cd44 variant region was isolated from a 129SV genomic library. Two 34 bp loxP sites were inserted in direct repeats into a single BstEII site 5' of exon v10 and at the 3' end of the neo resistance cassette, which was then inserted into the single BstXI site 3' of exon v10. The targeting vector was transfected in ES cells and homologous recombinant clones injected into C57BL/6 blastocysts. Chimaeric male offspring was then mated with C57BL/6 cre recombinase deleter females to allow the removal of the loxP flanked region. Offspring was genotyped by PCR using exon v10 flanking oligos and analysing for the deletion of the loxP targeted region. Ptprca (or Cd45.1 or Ly5.1): spontaneous mutation in the Ptprc (Cd45) gene, distinguishing it from Cd45.2 congenic strains. |
| Phenotypic information | Cd44v10-/-: Strongly reduced symptoms in autoimmune diseases and haemopoiesis. Ptprca (or Cd45.1 or Ly5.1): used as congenic marker for transplant studies. |
| Breeding history | 10 generations backcrossed to C57BL/6Rcc. Currently bred as C57BL/6Rcc Cd44v10-/- Ly5.1 |
| References |
|
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- T-B+ severe combined immunodeficiency due to CD45 deficiency / Orphanet_169157
MGI phenotypes (gene matching)
- decreased mast cell number / MGI
- altered response to myocardial infarction / MGI
- abnormal cell morphology / MGI
- decreased mast cell histamine storage / MGI
- abnormal uterus morphology / MGI
- thin epidermis / MGI
- abnormal lipid level / MGI
- abnormal vasculogenesis / MGI
- abnormal digestive system physiology / MGI
- impaired wound healing / MGI
- impaired macrophage phagocytosis / MGI
- liver inflammation / MGI
- lung inflammation / MGI
- abnormal definitive hematopoiesis / MGI
- no abnormal phenotype detected / MGI
- abnormal CD4-positive, alpha beta T cell morphology / MGI
- abnormal neutrophil physiology / MGI
- short tibia / MGI
- impaired skin barrier function / MGI
- delayed wound healing / MGI
- decreased myocardial infarction size / MGI
- abnormal leukocyte migration / MGI
- peritoneal inflammation / MGI
- decreased susceptibility to induced arthritis / MGI
- decreased tumor growth/size / MGI
- abnormal leukocyte adhesion / MGI
- abnormal physiological neovascularization / MGI
- increased susceptibility to induced arthritis / MGI
- enhanced coordination / MGI
- decreased cholesterol level / MGI
- abnormal vascular endothelial cell physiology / MGI
- decreased susceptibility to kidney reperfusion injury / MGI
- abnormal miniature excitatory postsynaptic currents / MGI
- decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
- increased mean systemic arterial blood pressure / MGI
- abnormal CD8-positive, alpha beta T cell morphology / MGI
- abnormal response to infection / MGI
- increased susceptibility to parasitic infection / MGI
- impaired natural killer cell mediated cytotoxicity / MGI
- decreased acute inflammation / MGI
- increased acute inflammation / MGI
- decreased T cell proliferation / MGI
- digestive/alimentary phenotype / MGI
- immune system phenotype / MGI
- hematopoietic system phenotype / MGI
- abnormal CD4-positive, alpha-beta T cell physiology / MGI
- abnormal T-helper 1 physiology / MGI
- abnormal T-helper 2 physiology / MGI
- choroidal neovascularization / MGI
- decreased circulating creatinine level / MGI
- decreased blood urea nitrogen level / MGI
- abnormal vascular smooth muscle physiology / MGI
- decreased angiogenesis / MGI
- decreased susceptibility to type IV hypersensitivity reaction / MGI
- abnormal cell physiology / MGI
- abnormal vascular endothelial cell morphology / MGI
- abnormal involution of the mammary gland / MGI
- increased diameter of tibia / MGI
- abnormal osteoclast differentiation / MGI
- increased circulating tumor necrosis factor level / MGI
- decreased circulating tumor necrosis factor level / MGI
- increased tumor necrosis factor secretion / MGI
- abnormal interleukin secretion / MGI
- increased circulating interferon-gamma level / MGI
- increased circulating interleukin-2 level / MGI
- increased interleukin-1 beta secretion / MGI
- increased interleukin-10 secretion / MGI
- increased interleukin-6 secretion / MGI
- impaired neutrophil recruitment / MGI
- abnormal chemokine secretion / MGI
- decreased transforming growth factor level / MGI
- decreased sensitivity to induced cell death / MGI
- abnormal epidermal lamellar body morphology / MGI
- increased sensitivity to induced morbidity/mortality / MGI
- abnormal circulating chemokine level / MGI
- abnormal circulating cytokine level / MGI
- decreased activation-induced cell death of T cells / MGI
- increased mitochondria size / MGI
- abnormal mitochondrial crista morphology / MGI
- decreased fibroblast proliferation / MGI
- impaired leukocyte tethering or rolling / MGI
- abnormal long bone metaphysis morphology / MGI
- abnormal leukocyte cell number / MGI
- increased neutrophil cell number / MGI
- decreased leukocyte cell number / MGI
- extramedullary hematopoiesis / MGI
- abnormal erythropoiesis / MGI
- enlarged spleen / MGI
- spleen hyperplasia / MGI
- enlarged lymph nodes / MGI
- decreased thymocyte number / MGI
- abnormal immune system cell morphology / MGI
- abnormal myelination / MGI
- abnormal oligodendrocyte morphology / MGI
- abnormal osteoclast physiology / MGI
- abnormal digestion / MGI
- abnormal immune system physiology / MGI
- abnormal humoral immune response / MGI
- thymus hypoplasia / MGI
- arrested T cell differentiation / MGI
- abnormal T cell activation / MGI
- liver inflammation / MGI
- lung inflammation / MGI
- premature death / MGI
- abnormal T cell differentiation / MGI
- no abnormal phenotype detected / MGI
- abnormal bone marrow cell morphology/development / MGI
- abnormal lymphopoiesis / MGI
- abnormal double-positive T cell morphology / MGI
- increased susceptibility to viral infection / MGI
- abnormal T cell physiology / MGI
- abnormal B cell number / MGI
- increased IgA level / MGI
- abnormal immune system organ morphology / MGI
- glomerulonephritis / MGI
- increased urine protein level / MGI
- abnormal cytokine secretion / MGI
- no phenotypic analysis / MGI
- kidney failure / MGI
- oliguria / MGI
- abnormal thymocyte activation / MGI
- abnormal T cell subpopulation ratio / MGI
- increased anti-double stranded DNA antibody level / MGI
- abnormal T cell selection / MGI
- abnormal positive T cell selection / MGI
- increased B-1 B cell number / MGI
- abnormal osteoclast morphology / MGI
- increased B cell number / MGI
- increased T cell number / MGI
- decreased B cell number / MGI
- decreased T cell number / MGI
- abnormal response to infection / MGI
- diarrhea / MGI
- decreased cytotoxic T cell cytolysis / MGI
- increased double-negative T cell number / MGI
- decreased double-positive T cell number / MGI
- decreased B cell proliferation / MGI
- decreased T cell proliferation / MGI
- increased susceptibility to autoimmune disorder / MGI
- immune system phenotype / MGI
- hematopoietic system phenotype / MGI
- decreased susceptibility to type I hypersensitivity reaction / MGI
- CNS inflammation / MGI
- increased T cell apoptosis / MGI
- abnormal T cell morphology / MGI
- increased NK cell number / MGI
- abnormal memory T cell number / MGI
- increased memory T cell number / MGI
- decreased memory T cell number / MGI
- absent T cells / MGI
- decreased CD4-positive, alpha beta T cell number / MGI
- abnormal CD4-positive T cell differentiation / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- abnormal CD8-positive, alpha-beta T cell differentiation / MGI
- increased single-positive T cell number / MGI
- decreased single-positive T cell number / MGI
- increased plasma cell number / MGI
- lymph node hyperplasia / MGI
- abnormal granulocyte differentiation / MGI
- increased dendritic cell number / MGI
- decreased B-1a cell number / MGI
- increased B-1b cell number / MGI
- decreased B-1b cell number / MGI
- decreased follicular B cell number / MGI
- increased transitional stage B cell number / MGI
- abnormal follicular B cell physiology / MGI
- decreased B-2 B cell number / MGI
- decreased mature B cell number / MGI
- abnormal B cell activation / MGI
- abnormal leukocyte morphology / MGI
- decreased gamma-delta intraepithelial T cell number / MGI
- abnormal CD4-positive, alpha-beta intraepithelial T cell morphology / MGI
- increased spleen red pulp amount / MGI
- increased spleen white pulp amount / MGI
- increased IgG2a level / MGI
- increased plasmacytoid dendritic cell number / MGI
- abnormal chemokine secretion / MGI
- decreased memory B cell number / MGI
- decreased mast cell degranulation / MGI
- abnormal intraepithelial T cell morphology / MGI
- abnormal intraepithelial T cell number / MGI
- decreased oligodendrocyte number / MGI
- increased DN3 thymocyte number / MGI
- decreased DN4 thymocyte number / MGI
- abnormal NK cell physiology / MGI
- mortality/aging / MGI
- decreased CD4-positive, alpha-beta memory T cell number / MGI
- decreased CD8-positive, alpha-beta memory T cell number / MGI
- lethality at weaning, incomplete penetrance / MGI
- increased alpha-beta T cell number / MGI
- decreased CD8-positive, naive alpha-beta T cell number / MGI
Literature references
- Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330