MGI phenotypes (allele matching)
B6Rcc.129-Cd44tm2.1Ugu/Cnrm
| Status | Available to order |
| EMMA ID | EM:01996 |
| International strain name | B6Rcc.129-Cd44tm2.1Ugu/Cnrm |
| Alternative name | C57BL/6RCC CD44v10-/- |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Cd44tm2.1Ugu |
| Gene/Transgene symbol | Cd44 |
Information from provider
| Provider | Ursula Günthert |
| Provider affiliation | Institut für Pathologie, University of Basel |
| Genetic information | The mouse Cd44 variant region was isolated from a 129SV genomic library. Two 34 bp loxP sites were inserted in direct repeats into a single BstEII site 5' of exon v10 and at the 3' end of the neo resistance cassette, which was then inserted into the single BstXI site 3' of exon v10. The targeting vector was transfected in ES cells and homologous recombinant clones injected into C57BL/6 blastocysts. Chimaeric male offspring was then mated with C57BL/6 cre recombinase deleter females to allow the removal of the loxP flanked region. Offspring was genotyped by PCR using exon v10 flanking oligos and analysing for the deletion of the loxP targeted region. |
| Phenotypic information | Cd44v10-/-: homozygous mice are viable and fertile, display strongly reduced symptoms in autoimmune diseases and defects in haemopoiesis. |
| Breeding history | 10 generations backcrossed to C57BL/6Rcc. Currently bred as C57BL/6Rcc Cd44v10-/- |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
MGI phenotypes (gene matching)
- decreased mast cell number / MGI
- altered response to myocardial infarction / MGI
- abnormal cell morphology / MGI
- decreased mast cell histamine storage / MGI
- abnormal uterus morphology / MGI
- thin epidermis / MGI
- abnormal lipid level / MGI
- abnormal vasculogenesis / MGI
- abnormal digestive system physiology / MGI
- impaired wound healing / MGI
- impaired macrophage phagocytosis / MGI
- liver inflammation / MGI
- lung inflammation / MGI
- abnormal definitive hematopoiesis / MGI
- no abnormal phenotype detected / MGI
- abnormal CD4-positive, alpha beta T cell morphology / MGI
- abnormal neutrophil physiology / MGI
- short tibia / MGI
- impaired skin barrier function / MGI
- delayed wound healing / MGI
- decreased myocardial infarction size / MGI
- abnormal leukocyte migration / MGI
- peritoneal inflammation / MGI
- decreased susceptibility to induced arthritis / MGI
- decreased tumor growth/size / MGI
- abnormal leukocyte adhesion / MGI
- abnormal physiological neovascularization / MGI
- increased susceptibility to induced arthritis / MGI
- enhanced coordination / MGI
- decreased cholesterol level / MGI
- abnormal vascular endothelial cell physiology / MGI
- decreased susceptibility to kidney reperfusion injury / MGI
- abnormal miniature excitatory postsynaptic currents / MGI
- decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
- increased mean systemic arterial blood pressure / MGI
- abnormal CD8-positive, alpha beta T cell morphology / MGI
- abnormal response to infection / MGI
- increased susceptibility to parasitic infection / MGI
- impaired natural killer cell mediated cytotoxicity / MGI
- decreased acute inflammation / MGI
- increased acute inflammation / MGI
- decreased T cell proliferation / MGI
- digestive/alimentary phenotype / MGI
- immune system phenotype / MGI
- hematopoietic system phenotype / MGI
- abnormal CD4-positive, alpha-beta T cell physiology / MGI
- abnormal T-helper 1 physiology / MGI
- abnormal T-helper 2 physiology / MGI
- choroidal neovascularization / MGI
- decreased circulating creatinine level / MGI
- decreased blood urea nitrogen level / MGI
- abnormal vascular smooth muscle physiology / MGI
- decreased angiogenesis / MGI
- decreased susceptibility to type IV hypersensitivity reaction / MGI
- abnormal cell physiology / MGI
- abnormal vascular endothelial cell morphology / MGI
- abnormal involution of the mammary gland / MGI
- increased diameter of tibia / MGI
- abnormal osteoclast differentiation / MGI
- increased circulating tumor necrosis factor level / MGI
- decreased circulating tumor necrosis factor level / MGI
- increased tumor necrosis factor secretion / MGI
- abnormal interleukin secretion / MGI
- increased circulating interferon-gamma level / MGI
- increased circulating interleukin-2 level / MGI
- increased interleukin-1 beta secretion / MGI
- increased interleukin-10 secretion / MGI
- increased interleukin-6 secretion / MGI
- impaired neutrophil recruitment / MGI
- abnormal chemokine secretion / MGI
- decreased transforming growth factor level / MGI
- decreased sensitivity to induced cell death / MGI
- abnormal epidermal lamellar body morphology / MGI
- increased sensitivity to induced morbidity/mortality / MGI
- abnormal circulating chemokine level / MGI
- abnormal circulating cytokine level / MGI
- decreased activation-induced cell death of T cells / MGI
- increased mitochondria size / MGI
- abnormal mitochondrial crista morphology / MGI
- decreased fibroblast proliferation / MGI
- impaired leukocyte tethering or rolling / MGI
Literature references
- Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330