B6;D2-Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey/Orl
| Status | Available to order |
| EMMA ID | EM:01971 |
| International strain name | B6;D2-Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey/Orl |
| Alternative name | Lk(TG)11 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey |
| Gene/Transgene symbol | Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey |
Information from provider
| Provider | Barbara Bertocci |
| Provider affiliation | INSERM 783 ex373 |
| Genetic information | The Lk(TG)11 transgene is based on the mouse immunoglobulin light chain Vkox-Jk5 rearranged transgene (Sharpe et al. 1991). A run of eleven TG dinucleotides was inserted in the KpnI restriction site at the border of the complementarity determining region 1 (CDR1) and framework region 2 (FR2) of the variable region and in HpaI site at the beginning of the constant region. The insertion of the dinucleotide repeats creates a STOP codon in the CDR2, rendering the transgene non-functional. |
| Phenotypic information | None |
| References |
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Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
Literature references
- Probing immunoglobulin gene hypermutation with microsatellites suggests a nonreplicative short patch DNA synthesis process.;Bertocci B, Quint L, Delbos F, Garcia C, Reynaud C A, Weill J C, ;1998;Immunity;9;257-65; 9729046