B6;D2-Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey/Orl
| Status | Available to order |
| EMMA ID | EM:01971 |
| Citation information | RRID:IMSR_EM:01971 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;D2-Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey/Orl |
| Alternative name | Lk(TG)11 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey |
| Gene/Transgene symbol | Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey |
Information from provider
| Provider | Barbara Bertocci |
| Provider affiliation | INSERM 783 ex373 |
| Genetic information | The Lk(TG)11 transgene is based on the mouse immunoglobulin light chain Vkox-Jk5 rearranged transgene (Sharpe et al. 1991). A run of eleven TG dinucleotides was inserted in the KpnI restriction site at the border of the complementarity determining region 1 (CDR1) and framework region 2 (FR2) of the variable region and in HpaI site at the beginning of the constant region. The insertion of the dinucleotide repeats creates a STOP codon in the CDR2, rendering the transgene non-functional. |
| Phenotypic information | None |
| References |
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Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
Literature references
- Probing immunoglobulin gene hypermutation with microsatellites suggests a nonreplicative short patch DNA synthesis process.;Bertocci B, Quint L, Delbos F, Garcia C, Reynaud C A, Weill J C, ;1998;Immunity;9;257-65; 9729046