MGI phenotypes (allele matching)
- no phenotypic analysis / MGI
B6;129P2-Gjd3tm1.1Kwi/Cnrm
| Status | Available to order |
| EMMA ID | EM:01915 |
| Citation information | RRID:IMSR_EM:01915 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129P2-Gjd3tm1.1Kwi/Cnrm |
| Alternative name | Cx30.2 flox |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Gjd3tm1.1Kwi |
| Gene/Transgene symbol | Gjd3 |
Information from provider
| Provider | Klaus WILLECKE |
| Provider affiliation | Molekulargenetik, Institut fuer Genetik, Universitaet Bonn |
| Genetic information | Instead of the whole open reading frame of exon 2 of Cx30.2 (Gjd3, formerly Gjc1) a loxP sites-flanked construct is inserted, containing the exon 2 of Cx30.2 and, 3' of the poly(A)-signal), one frt sites-flanked pgk-neomycin selection cassette. After the second loxP site a lacZ reporter gene cassette is inserted. After cre recombinase-mediated deletion of Cx30.2, lacZ is expressed under the control of the endogenous Cx30.2 promoter. |
| Phenotypic information | None in the loxP-flanked state. After cre recombinase-mediated deletion homozygous lacZ/lacZ mice are nearly normal. |
| Breeding history | After blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown coat colored offspring. This brown offspring was backcrossed to C57BL/6NCrl more than 3 times. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Backcrossed to C57BL/6J |
Disease and phenotype information
MGI phenotypes (gene matching)
- no phenotypic analysis / MGI
- abnormal impulse conducting system conduction / MGI
- nervous system phenotype / MGI
- cardiovascular system phenotype / MGI
- abnormal atrioventricular node conduction / MGI
- abnormal heart electrocardiography waveform feature / MGI
- shortened PR interval / MGI
- mortality/aging / MGI
- shortened PQ interval / MGI
Literature references
- Connexin30.2 containing gap junction channels decelerate impulse propagation through the atrioventricular node.;Kreuzberg Maria M, Schrickel Jan W, Ghanem Alexander, Kim Jung-Sun, Degen Joachim, Janssen-Bienhold Ulrike, Lewalter Thorsten, Tiemann Klaus, Willecke Klaus, ;2006;Proceedings of the National Academy of Sciences of the United States of America;103;5959-64; 16571663