B6;129S1-Tnftm1Ljo/Orl

Status

Available to order

EMMA IDEM:00186
International strain nameB6;129S1-Tnftm1Ljo/Orl
Alternative nameTNF-KO
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolTnftm1Ljo
Gene/Transgene symbolTnf

Information from provider

ProviderM Marino
Provider affiliationLudwig Institute for Cancer Research
Phenotypic informationTumor necrosis factor (TNF) plays a critical role in anti-tumor activity and proinflammatory actions. Tnf-/- mice are generated by a targeted mutation of the Tnf gene (insertion of a PGK-neo cassette and acquisition of a novel BamHI site). Tnf-/- mice develop normally and exhibit no consistent histological or morphological abnormalities. Sex ratios, litter size and weight gain of Tnf-/- mice are similar to Tnf+/+ littermates.
References
  • Characterization of tumor necrosis factor-deficient mice.;Marino M W, Dunn A, Grail D, Inglese M, Noguchi Y, Richards E, Jungbluth A, Wada H, Moore M, Williamson B, Basu S, Old L J, ;1997;Proceedings of the National Academy of Sciences of the United States of America;94;8093-8; 9223320

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

MGI phenotypes (allele matching)
  • increased susceptibility to bacterial infection / MGI
  • increased susceptibility to fungal infection / MGI
  • abnormal cerebral cortex morphology / MGI
  • abnormal lung morphology / MGI
  • impaired coordination / MGI
  • abnormal motor coordination/ balance / MGI
  • lung inflammation / MGI
  • decreased apoptosis / MGI
  • abnormal long term spatial reference memory / MGI
  • altered susceptibility to infection / MGI
  • decreased IgG level / MGI
  • abnormal cytokine secretion / MGI
  • decreased susceptibility to autoimmune disorder / MGI
  • abnormal follicular dendritic cell physiology / MGI
  • abnormal spleen B cell follicle morphology / MGI
  • absent spleen germinal center / MGI
  • decreased spleen white pulp amount / MGI
  • decreased circulating tumor necrosis factor level / MGI
  • abnormal chemokine secretion / MGI
  • decreased susceptibility to endotoxin shock / MGI
  • liver/biliary system phenotype / MGI
  • increased neutrophil cell number / MGI
  • skin lesions / MGI
  • decreased immunoglobulin level / MGI
  • abnormal neutrophil physiology / MGI
  • skin inflammation / MGI
  • increased susceptibility to parasitic infection / MGI
  • lymph node hypoplasia / MGI
  • increased interferon-gamma secretion / MGI
  • abnormal lymph node cell ratio / MGI
  • increased leukocyte cell number / MGI
  • abnormal Peyer's patch follicle morphology / MGI
  • abnormal Peyer's patch germinal center morphology / MGI
  • abnormal Peyer's patch T cell area morphology / MGI
  • decreased susceptibility to bacterial infection / MGI
  • increased lymphocyte cell number / MGI
  • decreased Peyer's patch number / MGI
  • small Peyer's patches / MGI
  • absent follicular dendritic cells / MGI
MGI phenotypes (gene matching)
  • increased bone mineral density / MGI
  • abnormal trabecular bone morphology / MGI
  • increased leukocyte cell number / MGI
  • increased neutrophil cell number / MGI
  • increased cell proliferation / MGI
  • alopecia / MGI
  • enlarged liver / MGI
  • abnormal liver physiology / MGI
  • abnormal spleen morphology / MGI
  • enlarged spleen / MGI
  • abnormal Peyer's patch morphology / MGI
  • abnormal cerebral cortex morphology / MGI
  • abnormal spermatogenesis / MGI
  • abnormal lung morphology / MGI
  • skin lesions / MGI
  • increased body length / MGI
  • increased body weight / MGI
  • decreased body weight / MGI
  • increased anxiety-related response / MGI
  • hypoactivity / MGI
  • impaired coordination / MGI
  • increased grooming behavior / MGI
  • abnormal sleep pattern / MGI
  • abnormal motor coordination/balance / MGI
  • abnormal myelopoiesis / MGI
  • abnormal immune system physiology / MGI
  • altered susceptibility to infection / MGI
  • impaired macrophage phagocytosis / MGI
  • abnormal humoral immune response / MGI
  • decreased IgG level / MGI
  • decreased IgA level / MGI
  • thymus hypoplasia / MGI
  • autoimmune response / MGI
  • abnormal inflammatory response / MGI
  • intestinal inflammation / MGI
  • lung inflammation / MGI
  • decreased inflammatory response / MGI
  • abnormal glucose homeostasis / MGI
  • premature death / MGI
  • abnormal lipid homeostasis / MGI
  • abnormal lymph node B cell domain morphology / MGI
  • abnormal lymph node primary follicle morphology / MGI
  • abnormal spleen white pulp morphology / MGI
  • abnormal spleen marginal zone morphology / MGI
  • abnormal spleen marginal sinus morphology / MGI
  • abnormal Peyer's patch follicle morphology / MGI
  • abnormal Peyer's patch germinal center morphology / MGI
  • abnormal Peyer's patch T cell area morphology / MGI
  • decreased susceptibility to bacterial infection / MGI
  • increased susceptibility to bacterial infection / MGI
  • increased susceptibility to viral infection / MGI
  • abnormal T cell physiology / MGI
  • abnormal macrophage physiology / MGI
  • decreased immunoglobulin level / MGI
  • abnormal neutrophil physiology / MGI
  • increased IgM level / MGI
  • hepatic steatosis / MGI
  • decreased circulating triglyceride level / MGI
  • oligozoospermia / MGI
  • enhanced wound healing / MGI
  • decreased circulating insulin level / MGI
  • abnormal aortic valve morphology / MGI
  • decreased vertical activity / MGI
  • abnormal testes secretion / MGI
  • steatorrhea / MGI
  • absent Peyer's patches / MGI
  • increased heart weight / MGI
  • abnormal joint morphology / MGI
  • increased liver weight / MGI
  • arthritis / MGI
  • abnormal cytokine secretion / MGI
  • no phenotypic analysis / MGI
  • decreased coping response / MGI
  • cardiac fibrosis / MGI
  • prolonged QT interval / MGI
  • small intestinal inflammation / MGI
  • decreased cardiac output / MGI
  • decreased susceptibility to induced arthritis / MGI
  • rheumatoid arthritis / MGI
  • abnormal female reproductive system physiology / MGI
  • abnormal T cell subpopulation ratio / MGI
  • abnormal lymphocyte physiology / MGI
  • abnormal spermiation / MGI
  • long tibia / MGI
  • decreased incidence of tumors by chemical induction / MGI
  • decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
  • abnormal class switch recombination / MGI
  • decreased testis weight / MGI
  • abnormal testis physiology / MGI
  • skin inflammation / MGI
  • increased spleen weight / MGI
  • increased osteoblast cell number / MGI
  • abnormal CD8-positive, alpha beta T cell morphology / MGI
  • increased lymphocyte cell number / MGI
  • increased B cell number / MGI
  • decreased T cell number / MGI
  • decreased susceptibility to parasitic infection / MGI
  • increased susceptibility to parasitic infection / MGI
  • abnormal cytotoxic T cell physiology / MGI
  • increased susceptibility to injury / MGI
  • abnormal mesenteric lymph node morphology / MGI
  • decreased heart rate / MGI
  • abnormal fat pad morphology / MGI
  • decreased susceptibility to autoimmune disorder / MGI
  • liver/biliary system phenotype / MGI
  • immune system phenotype / MGI
  • increased susceptibility to fungal infection / MGI
  • abnormal food intake / MGI
  • decreased percent body fat/body weight / MGI
  • abnormal CD4-positive, alpha-beta T cell physiology / MGI
  • decreased circulating glucose level / MGI
  • decreased ventricle muscle contractility / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • increased susceptibility to diet-induced obesity / MGI
  • decreased susceptibility to diet-induced obesity / MGI
  • abnormal circulating leptin level / MGI
  • decreased circulating leptin level / MGI
  • abnormal response to transplant / MGI
  • increased apoptosis / MGI
  • decreased apoptosis / MGI
  • increased body mass index / MGI
  • aortic valve stenosis / MGI
  • absent lymph nodes / MGI
  • increased CD4-positive, alpha beta T cell number / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • lymph node hypoplasia / MGI
  • increased dendritic cell number / MGI
  • decreased Peyer's patch number / MGI
  • small Peyer's patches / MGI
  • absent germinal center B cells / MGI
  • abnormal follicular dendritic cell physiology / MGI
  • decreased follicular dendritic cell number / MGI
  • absent follicular dendritic cells / MGI
  • absent spleen marginal zone / MGI
  • abnormal metallophilic macrophage morphology / MGI
  • abnormal gamma-delta T cell morphology / MGI
  • abnormal alpha-beta intraepithelial T cell morphology / MGI
  • abnormal long term spatial reference memory / MGI
  • absent peripheral lymph nodes / MGI
  • absent mesenteric lymph nodes / MGI
  • abnormal spleen B cell follicle morphology / MGI
  • abnormal spleen primary B follicle morphology / MGI
  • abnormal spleen follicular dendritic cell network / MGI
  • absent spleen germinal center / MGI
  • intermingled spleen red and white pulp / MGI
  • decreased spleen white pulp amount / MGI
  • decreased spleen germinal center number / MGI
  • decreased spleen germinal center size / MGI
  • decreased IgG1 level / MGI
  • decreased IgG2a level / MGI
  • decreased IgG3 level / MGI
  • absent lymph node germinal center / MGI
  • increased circulating tumor necrosis factor level / MGI
  • decreased circulating tumor necrosis factor level / MGI
  • increased tumor necrosis factor secretion / MGI
  • decreased tumor necrosis factor secretion / MGI
  • increased interferon-gamma secretion / MGI
  • decreased interferon-gamma secretion / MGI
  • decreased circulating interferon-gamma level / MGI
  • decreased circulating interleukin-6 level / MGI
  • decreased circulating interleukin-1 alpha level / MGI
  • decreased circulating interleukin-1 beta level / MGI
  • increased interleukin-2 secretion / MGI
  • abnormal cytokine level / MGI
  • abnormal chemokine secretion / MGI
  • decreased susceptibility to endotoxin shock / MGI
  • increased susceptibility to endotoxin shock / MGI
  • abnormal lymph node cell ratio / MGI
  • abnormal vagina weight / MGI
  • prolonged estrus / MGI
  • ileum inflammation / MGI
  • decreased keratinocyte proliferation / MGI
  • absent inguinal lymph nodes / MGI
  • absent brachial lymph nodes / MGI
  • absent axillary lymph nodes / MGI
  • absent cervical lymph nodes / MGI
  • absent popliteal lymph nodes / MGI
  • increased sensitivity to induced morbidity/mortality / MGI
  • decreased sensitivity to induced morbidity/mortality / MGI
  • increased susceptibility to bacterial infection induced morbidity/mortality / MGI
  • increased serotonin level / MGI
  • abnormal intestine physiology / MGI
  • abnormal circulating chemokine level / MGI
  • abnormal T-helper 17 cell physiology / MGI
  • decreased liver free fatty acids level / MGI
  • thick aortic valve / MGI
  • increased CD4-positive, alpha-beta memory T cell number / MGI
  • decreased macrophage cytokine production / MGI
  • abnormal aorta bulb morphology / MGI
  • increased fibroblast proliferation / MGI
  • increased fibroblast cell migration / MGI
  • increased cell migration / MGI
  • abnormal aortic valve flow / MGI
  • alveolar process atrophy / MGI

Literature references

  • Characterization of tumor necrosis factor-deficient mice.;Marino M W, Dunn A, Grail D, Inglese M, Noguchi Y, Richards E, Jungbluth A, Wada H, Moore M, Williamson B, Basu S, Old L J, ;1997;Proceedings of the National Academy of Sciences of the United States of America;94;8093-8; 9223320

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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