MGI phenotypes (allele matching)
B6.129-Rasd2tm1Rdl/Cnrm
| Status | Available to order |
| EMMA ID | EM:01823 |
| Citation information | RRID:IMSR_EM:01823 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129-Rasd2tm1Rdl/Cnrm |
| Alternative name | RHES KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Rasd2tm1Rdl |
| Gene/Transgene symbol | Rasd2 |
Information from provider
| Provider | Roberto DI LAURO |
| Provider affiliation | BioGeM |
| Genetic information | The Rasd2 (or Rhes, Ras homolog enriched in striatum) gene encodes for a 266-aminoacid protein, predominantly expressed in the striatum, that belongs to the RASD subfamily of the Ras-related GTP-binding protein superfamily. We generated mice carrying null mutations in the Rhes gene by gene-targeting techniques. The Rhes genomic locus was isolated by genotyping screening of the phage artificial chromosome library RPCI-21 (provided by the Yac Screening Center, DIBIT-HSR and IGBE/IGM-CNR, Milan, Italy). The internal ribosome entry site-EGFP cassette and the PGKneo cassette, flanked by loxP sequences, were flanked by two Rhes genomic DNA fragments: a 2.9 kb fragment including the Rhes translational start codon and a 5 kb fragment. The 2.9 kb genomic fragment underwent a site-directed mutagenesis reaction which allowed insertion of a stop codon in each reading frame and EcoRI and XhoI restriction sites downstream of the Rhes translational start codon. |
| Phenotypic information | Mice homozygous for the Rasd2 (Rhes) mutation are viable and fertile but smaller than wild-type mice. Furthermore, they show a gender-dependent increase in anxiety levels and a motor coordination deficit but no learning or memory impairment. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Literature references
- Rhes is involved in striatal function.;Spano Daniela, Branchi Igor, Rosica Annamaria, Pirro Maria Teresa, Riccio Antonio, Mithbaokar Pratibha, Affuso Andrea, Arra Claudio, Campolongo Patrizia, Terracciano Daniela, Macchia Vincenzo, Bernal Juan, Alleva Enrico, Di Lauro Roberto, ;2004;Molecular and cellular biology;24;5788-96; 15199135