C3El.Cg-Gpi1^b-m1Neu/Ieg

Status	Available to order
EMMA ID	EM:01816
Citation information	RRID:IMSR_EM:01816 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	C3El.Cg-Gpi1^b-m1Neu/Ieg
Alternative name	GPI4314
Strain type	Induced Mutant Strains : Chemically-induced
Allele/Transgene symbol	Gpi1^b-m1Neu
Gene/Transgene symbol	Gpi1

Information from provider

Provider	Walter Pretsch
Provider affiliation	Institute of Experimental Genetics, Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH)
Genetic information	Point mutation GTG -> GAG resulting in an amino acid alteration Val -> Glu at position 118.
Phenotypic information	Glucose-6-phosphate isomerase deficiency (approximately 60% residual activity in heterozygous and approximately 20% residual activity in homozygous mutants) in blood and other organs.
Breeding history	C3H/El (47th generation).
References	Linear dose-response relationship of erythrocyte enzyme-activity mutations in offspring of ethylnitrosourea-treated mice.;Charles D J, Pretsch W, ;1987;Mutation research;176;81-91; 3796661 Glucose phosphate isomerase enzyme-activity mutants in Mus musculus: genetical and biochemical characterization.;Pretsch W, Merkle S, ;1990;Biochemical genetics;28;97-110; 2344351 Glucose-6-phosphate isomerase deficiency associated with nonspherocytic hemolytic anemia in the mouse: an animal model for the human disease.;Merkle S, Pretsch W, ;1993;Blood;81;206-13; 8417789

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

MGI allele-associated human disease models

congenital nonspherocytic hemolytic anemia / DOID:2861

MGI phenotypes (allele matching)

decreased hematocrit / MGI
decreased body weight / MGI
abnormal erythrocyte morphology / MGI
increased mean corpuscular volume / MGI
reticulocytosis / MGI
increased heart weight / MGI
decreased erythrocyte cell number / MGI
increased liver weight / MGI
abnormal erythrocyte physiology / MGI
abnormal erythrocyte osmotic lysis / MGI
increased spleen weight / MGI
increased circulating bilirubin level / MGI
increased circulating glucose level / MGI
decreased mean corpuscular hemoglobin / MGI
abnormal enzyme/coenzyme activity / MGI
decreased mean corpuscular hemoglobin concentration / MGI
lethality, incomplete penetrance / MGI
postnatal lethality, incomplete penetrance / MGI

MGI phenotypes (gene matching)

decreased hematocrit / MGI
decreased body weight / MGI
prenatal lethality / MGI
abnormal embryonic tissue morphology / MGI
abnormal erythrocyte morphology / MGI
increased mean corpuscular volume / MGI
reticulocytosis / MGI
increased heart weight / MGI
decreased erythrocyte cell number / MGI
increased liver weight / MGI
abnormal erythrocyte physiology / MGI
abnormal erythrocyte osmotic lysis / MGI
increased spleen weight / MGI
increased circulating bilirubin level / MGI
increased circulating glucose level / MGI
decreased mean corpuscular hemoglobin / MGI
abnormal enzyme/coenzyme activity / MGI
increased lung weight / MGI
decreased mean corpuscular hemoglobin concentration / MGI
lethality, incomplete penetrance / MGI
postnatal lethality, incomplete penetrance / MGI
prenatal lethality, complete penetrance / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality between implantation and placentation, complete penetrance / MGI
preweaning lethality, complete penetrance / MGI

Literature references

Linear dose-response relationship of erythrocyte enzyme-activity mutations in offspring of ethylnitrosourea-treated mice.;Charles D J, Pretsch W, ;1987;Mutation research;176;81-91; 3796661
Glucose phosphate isomerase enzyme-activity mutants in Mus musculus: genetical and biochemical characterization.;Pretsch W, Merkle S, ;1990;Biochemical genetics;28;97-110; 2344351
Glucose-6-phosphate isomerase deficiency associated with nonspherocytic hemolytic anemia in the mouse: an animal model for the human disease.;Merkle S, Pretsch W, ;1993;Blood;81;206-13; 8417789

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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C3El.Cg-Gpi1b-m1Neu/Ieg