CD1(B6)-Tg(Hoxb1-cre)r4Mist/Cnrm
| Status | Available to order |
| EMMA ID | EM:01796 |
| International strain name | CD1(B6)-Tg(Hoxb1-cre)r4Mist/Cnrm |
| Alternative name | R4-CRE |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Hoxb1-cre)r4Mist |
| Gene/Transgene symbol | Tg(Hoxb1-cre)r4Mist |
Information from provider
| Provider | Michèle Studer |
| Provider affiliation | TIGEM (Telethon Institute of Genetics and Medicine) |
| Genetic information | Generation of the rhombomere (r)4-cre transgenic line: A 2,7 kb XhoI-HindIII construct containing the cre recombinase gene and the metallothionein-1 polyadenylation signal obtained from pBS185 (GibcoBRL, Cat# 10347-011) was cloned into EcoRV -SacII of pWexP3β (Danielian and McMahon 1996; gift from Paul S. Danielian), which includes a Wnt1 basic promoter. The 741 bp ApaI-SpeI fragment containing the Hoxb1 r3/r5 repressor and the 1043 bp SpeI-SmaI fragment containing the Hoxb1 5' r4 enhancer were cloned inverted (Studer et al. 1994) and inserted into the BglII of the above plasmid. This combination leads to restricted expression of cre recombinase in rhombomere 4 of developing embryo hindbrain. The targeting construct was digested with NotI and injected into pronuclei of CD1 oocytes. |
| Phenotypic information | None. |
| Breeding history | The strain was maintained on a C57BL/6 background. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Original background CD1, then backcrossed to B6 and further backcrossed less than 3 times to CD1 |
Literature references
- Role of a conserved retinoic acid response element in rhombomere restriction of Hoxb-1.;Studer M, Pöpperl H, Marshall H, Kuroiwa A, Krumlauf R, ;1994;Science (New York, N.Y.);265;1728-32; 7916164