STOCK Axin2^tm1Wbm/Ieg

Status	Available to order
EMMA ID	EM:01789
International strain name	STOCK Axin2^tm1Wbm/Ieg
Alternative name	Conductin (Axin2)-lacZ
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Axin2^tm1Wbm
Gene/Transgene symbol	Axin2

Information from provider

Provider	Boris Jerchow
Provider affiliation	Max-Delbrueck-Center for Molecular Medicine - HGF
Genetic information	Axin2 null mutation. Knock-in of a NLS-lacZ cassette in frame with the ATG start codon of Axin2. Antisense PGK-neo cassette flanked by loxP-sites following the lacZ. Only splice acceptor and donor of first coding exon (exon 2) left.
Phenotypic information	Minor defect: craniosynosthosis.
Breeding history	Backcrossed to C57BL/6. Number of generations unknown. Currently backcrossed to C57BL/6 and mated to (C57BL/6 x DBA/2)F1 hybrids.
References	The role of Axin2 in calvarial morphogenesis and craniosynostosis.;Yu Hsiao-Man Ivy, Jerchow Boris, Sheu Tzong-Jen, Liu Bo, Costantini Frank, Puzas J Edward, Birchmeier Walter, Hsu Wei, ;2005;Development (Cambridge, England);132;1995-2005; 15790973 Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and liver tumors.;Lustig Barbara, Jerchow Boris, Sachs Martin, Weiler Sigrid, Pietsch Torsten, Karsten Uwe, van de Wetering Marc, Clevers Hans, Schlag Peter M, Birchmeier Walter, Behrens Jürgen, ;2002;Molecular and cellular biology;22;1184-93; 11809809

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

MGI allele-associated human disease models

craniosynostosis / DOID:2340

Orphanet associated rare diseases, based on orthologous gene matching

AXIN2-related attenuated familial adenomatous polyposis / Orphanet_401911
Oligodontia / Orphanet_99798
Oligodontia-cancer predisposition syndrome / Orphanet_300576

IMPC phenotypes (gene matching)

polydactyly / IMPC
preweaning lethality, complete penetrance / IMPC
abnormal head shape / IMPC
abnormal embryo size / IMPC
protruding tongue / IMPC
abnormal limb morphology / IMPC
microphthalmia / IMPC
unresponsive to tactile stimuli / IMPC
abnormal eyelid fusion / IMPC
abnormal facial morphology / IMPC
abnormal skin coloration / IMPC
abnormal embryo development / IMPC
abnormal body wall morphology / IMPC
anophthalmia / IMPC

MGI phenotypes (allele matching)

abnormal neurocranium morphology / MGI
shortened head / MGI
abnormal cranium morphology / MGI
abnormal eye morphology / MGI
abnormal skeleton development / MGI
premature intramembranous bone ossification / MGI
abnormal osteoblast physiology / MGI
decreased cranium height / MGI
enhanced osteoblast differentiation / MGI
premature metopic suture closure / MGI
absent jugum limitans / MGI
increased osteoblast proliferation / MGI

MGI phenotypes (gene matching)

abnormal neurocranium morphology / MGI
abnormal heart morphology / MGI
shortened head / MGI
abnormal cranium morphology / MGI
kinked tail / MGI
exencephaly / MGI
open neural tube / MGI
embryonic growth arrest / MGI
postnatal growth retardation / MGI
abnormal eye morphology / MGI
abnormal tail morphology / MGI
abnormal skeleton development / MGI
no abnormal phenotype detected / MGI
abnormal primitive streak morphology / MGI
no phenotypic analysis / MGI
curly tail / MGI
premature intramembranous bone ossification / MGI
cardia bifida / MGI
abnormal osteoblast physiology / MGI
abnormal neural plate morphology / MGI
abnormal tail development / MGI
decreased cranium height / MGI
prenatal lethality, incomplete penetrance / MGI
embryonic lethality during organogenesis, incomplete penetrance / MGI
enhanced osteoblast differentiation / MGI
premature metopic suture closure / MGI
absent jugum limitans / MGI
increased osteoblast proliferation / MGI

Literature references

The role of Axin2 in calvarial morphogenesis and craniosynostosis.;Yu Hsiao-Man Ivy, Jerchow Boris, Sheu Tzong-Jen, Liu Bo, Costantini Frank, Puzas J Edward, Birchmeier Walter, Hsu Wei, ;2005;Development (Cambridge, England);132;1995-2005; 15790973
Negative feedback loop of Wnt signaling through upregulation of conductin/axin2 in colorectal and liver tumors.;Lustig Barbara, Jerchow Boris, Sachs Martin, Weiler Sigrid, Pietsch Torsten, Karsten Uwe, van de Wetering Marc, Clevers Hans, Schlag Peter M, Birchmeier Walter, Behrens Jürgen, ;2002;Molecular and cellular biology;22;1184-93; 11809809
In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration.;Huch Meritxell, Dorrell Craig, Boj Sylvia F, van Es Johan H, Li Vivian S W, van de Wetering Marc, Sato Toshiro, Hamer Karien, Sasaki Nobuo, Finegold Milton J, Haft Annelise, Vries Robert G, Grompe Markus, Clevers Hans, ;2013;Nature;494;247-50; 23354049
Unlimited in vitro expansion of adult bi-potent pancreas progenitors through the Lgr5/R-spondin axis.;Huch Meritxell, Bonfanti Paola, Boj Sylvia F, Sato Toshiro, Loomans Cindy J M, van de Wetering Marc, Sojoodi Mozhdeh, Li Vivian S W, Schuijers Jurian, Gracanin Ana, Ringnalda Femke, Begthel Harry, Hamer Karien, Mulder Joyce, van Es Johan H, de Koning Eelco, Vries Robert G J, Heimberg Harry, Clevers Hans, ;2013;The EMBO journal;32;2708-21; 24045232
Beta-catenin/T-cell factor signaling is activated during lung injury and promotes the survival and migration of alveolar epithelial cells.;Flozak Annette S, Lam Anna P, Russell Susan, Jain Manu, Peled Ofra N, Sheppard Kerry A, Beri Rohinee, Mutlu Gökhan M, Budinger G R Scott, Gottardi Cara J, ;2010;The Journal of biological chemistry;285;3157-67; 19933277
Activation of Wnt signaling promotes hippocampal neurogenesis in experimental autoimmune encephalomyelitis.;Schneider Reiner, Koop Barbara, Schröter Friederike, Cline Jason, Ingwersen Jens, Berndt Carsten, Hartung Hans-Peter, Aktas Orhan, Prozorovski Tim, ;2016;Molecular neurodegeneration;11;53; 27480121

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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STOCK Axin2tm1Wbm/Ieg