B6.129P2-Mapk8^tm1Wag/Kctt

Status	Available to order
EMMA ID	EM:01772
International strain name	B6.129P2-Mapk8^tm1Wag/Kctt
Alternative name	Jnk1-C57Bl6
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Mapk8^tm1Wag
Gene/Transgene symbol	Mapk8

Information from provider

Provider	Ingrid Fossar-Larsen
Provider affiliation	Department of Molecular Cancer Biology, Danish Cancer Society
Genetic information	The Mapk8 (Jnk1) gene is disrupted by insertion of the lacZ cassette at the first exon. This mutation inactivates all known Mapk8 splicing variants.
Phenotypic information	No major defects. Defects in T-cell apoptosis and proliferation. Mice are resistant to type 2 diabetes.
References	c-Jun NH2-terminal kinase (JNK)1 and JNK2 have similar and stage-dependent roles in regulating T cell apoptosis and proliferation.;Sabapathy K, Kallunki T, David J P, Graef I, Karin M, Wagner E F, ;2001;The Journal of experimental medicine;193;317-28; 11157052 A central role for JNK in obesity and insulin resistance.;Hirosumi Jiro, Tuncman Gürol, Chang Lufen, Görgün Cem Z, Uysal K Teoman, Maeda Kazuhisa, Karin Michael, Hotamisligil Gökhan S, ;2002;Nature;420;333-6; 12447443 JNK1 is required for maintenance of neuronal microtubules and controls phosphorylation of microtubule-associated proteins.;Chang Lufen, Jones Ying, Ellisman Mark H, Goldstein Lawrence S B, Karin Michael, ;2003;Developmental cell;4;521-33; 12689591 Constitutively active cytoplasmic c-Jun N-terminal kinase 1 is a dominant regulator of dendritic architecture: role of microtubule-associated protein 2 as an effector.;Björkblom Benny, Ostman Nina, Hongisto Vesa, Komarovski Vladislav, Filén Jan-Jonas, Nyman Tuula A, Kallunki Tuula, Courtney Michael J, Coffey Eleanor T, ;2005;The Journal of neuroscience : the official journal of the Society for Neuroscience;25;6350-61; 16000625

Information from EMMA

Archiving centre	Karolinska Institutet, Stockholm, Sweden

Disease and phenotype information

MGI phenotypes (allele matching)

decreased circulating insulin level / MGI
increased insulin sensitivity / MGI
improved glucose tolerance / MGI
decreased circulating glucose level / MGI
decreased susceptibility to diet-induced obesity / MGI
decreased subcutaneous adipose tissue amount / MGI
abnormal fat cell morphology / MGI
increased epididymal fat pad weight / MGI
decreased total body fat amount / MGI
abnormal T cell activation / MGI
decreased T cell proliferation / MGI
decreased T cell apoptosis / MGI

MGI phenotypes (gene matching)

thin epidermis / MGI
abnormal epidermis stratum basale morphology / MGI
decreased body length / MGI
decreased body weight / MGI
abnormal T cell activation / MGI
decreased circulating insulin level / MGI
increased insulin sensitivity / MGI
abnormal cytokine secretion / MGI
decreased insulin secretion / MGI
cardiac fibrosis / MGI
increased cardiomyocyte apoptosis / MGI
decreased cardiomyocyte apoptosis / MGI
increased response of heart to induced stress / MGI
increased incidence of tumors by chemical induction / MGI
decreased T cell proliferation / MGI
decreased cardiac muscle contractility / MGI
improved glucose tolerance / MGI
abnormal myocardium layer morphology / MGI
increased T cell proliferation / MGI
liver/biliary system phenotype / MGI
homeostasis/metabolism phenotype / MGI
decreased percent body fat/body weight / MGI
decreased circulating glucose level / MGI
abnormal enzyme/coenzyme activity / MGI
decreased susceptibility to diet-induced obesity / MGI
decreased circulating leptin level / MGI
decreased T cell apoptosis / MGI
abnormal T-helper 1 cell differentiation / MGI
decreased subcutaneous adipose tissue amount / MGI
abnormal fat cell morphology / MGI
decreased gonadal fat pad weight / MGI
increased epididymal fat pad weight / MGI
absent keratohyalin granules / MGI
decreased total body fat amount / MGI
increased respiratory quotient / MGI
decreased food intake / MGI

Literature references

c-Jun NH2-terminal kinase (JNK)1 and JNK2 have similar and stage-dependent roles in regulating T cell apoptosis and proliferation.;Sabapathy K, Kallunki T, David J P, Graef I, Karin M, Wagner E F, ;2001;The Journal of experimental medicine;193;317-28; 11157052
A central role for JNK in obesity and insulin resistance.;Hirosumi Jiro, Tuncman Gürol, Chang Lufen, Görgün Cem Z, Uysal K Teoman, Maeda Kazuhisa, Karin Michael, Hotamisligil Gökhan S, ;2002;Nature;420;333-6; 12447443
JNK1 is required for maintenance of neuronal microtubules and controls phosphorylation of microtubule-associated proteins.;Chang Lufen, Jones Ying, Ellisman Mark H, Goldstein Lawrence S B, Karin Michael, ;2003;Developmental cell;4;521-33; 12689591
Constitutively active cytoplasmic c-Jun N-terminal kinase 1 is a dominant regulator of dendritic architecture: role of microtubule-associated protein 2 as an effector.;Björkblom Benny, Ostman Nina, Hongisto Vesa, Komarovski Vladislav, Filén Jan-Jonas, Nyman Tuula A, Kallunki Tuula, Courtney Michael J, Coffey Eleanor T, ;2005;The Journal of neuroscience : the official journal of the Society for Neuroscience;25;6350-61; 16000625

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6.129P2-Mapk8tm1Wag/Kctt