C57BL/6J-Apc^Min/5H

Status	Available to order
EMMA ID	EM:01724
International strain name	C57BL/6J-Apc^Min/5H
Alternative name	Apc<Min>, Line V
Strain type	Induced Mutant Strains : Chemically-induced
Allele/Transgene symbol	Apc^Min
Gene/Transgene symbol	Apc

Information from provider

Provider	Michelle Ellender
Provider affiliation	Health Protection Agency, Chilton, Didcot, Oxon OX11 0RQ
Genetic information	Intestinal polyposis (high multiplicity). The mutation is on chromosome 18 and is a transversion point mutation that alters nucleotide 2549 from a T to an A (mRNA: NM_001360980.1; protein: NM_007462.3). This converts codon 850 from one encoding a leucine to a stop codon (p.Leu850*), truncating the expected polypeptide. The Apc mutation is the homologue of the human APC (adenomatous polyposis coli) gene and the Min (multiple intestinal neoplasia) mice are highly susceptible to spontaneous intestinal tumour formation. This line (#5 or V) is distinct from the other Apc^Min line (#2 or II) also submitted to EMMA (strain ID EM:01715). These different Apc^Min lines have been described as having different severity of phenotype (Haines J et al., PNAS, 2005, doi: 10.1073/pnas.0500039102).
Phenotypic information	Mice develop life-shortening intestinal polyposis.
References	A dominant mutation that predisposes to multiple intestinal neoplasia in the mouse.;Moser A R, Pitot H C, Dove W F, ;1990;Science (New York, N.Y.);247;322-4; 2296722 Genetic basis of variation in adenoma multiplicity in ApcMin/+ Mom1S mice.;Haines Jackie, Johnson Victoria, Pack Kevin, Suraweera Nirosha, Slijepcevic Predrag, Cabuy Erik, Coster Margaret, Ilyas Mohammad, Wilding Jennifer, Sieber Oliver, Bodmer Walter, Tomlinson Ian, Silver Andrew, ;2005;Proceedings of the National Academy of Sciences of the United States of America;102;2868-73; 15710876

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

Desmoid tumor / Orphanet_873
Cenani-Lenz syndrome / Orphanet_3258
Turcot syndrome with polyposis / Orphanet_99818
Gastric adenocarcinoma and proximal polyposis of the stomach / Orphanet_314022
Gardner syndrome / Orphanet_79665
APC-related attenuated familial adenomatous polyposis / Orphanet_247806

MGI phenotypes (allele matching)

abnormal mammary gland development / MGI
abnormal ovarian folliculogenesis / MGI
abnormal ovarian follicle morphology / MGI
absent mature ovarian follicles / MGI
absent corpus luteum / MGI
abnormal vagina epithelium morphology / MGI
decreased body weight / MGI
increased incidence of induced tumors / MGI
ovary atrophy / MGI
uterus atrophy / MGI
reproductive system phenotype / MGI
adrenal gland hyperplasia / MGI
absent nipple / MGI
increased mammary gland tumor incidence / MGI
mammary gland hyperplasia / MGI
anemia / MGI
premature death / MGI
increased intestinal adenoma incidence / MGI
increased intestinal adenocarcinoma incidence / MGI
abnormal pregnancy / MGI
extended life span / MGI
aneuploidy / MGI
increased incidence of tumors by chemical induction / MGI
decreased intestinal adenoma incidence / MGI
decreased hematocrit / MGI
hyperlipidemia / MGI
melena / MGI
rectal prolapse / MGI
abnormal intestinal mucosa morphology / MGI
increased mammary adenocarcinoma incidence / MGI
intestinal obstruction / MGI
abnormal intestinal goblet cell morphology / MGI
abnormal large intestine crypts of Lieberkuhn morphology / MGI
intestine polyps / MGI
abnormal enterocyte proliferation / MGI
decreased T cell number / MGI
decreased NK cell number / MGI
decreased splenocyte number / MGI
neoplasm / MGI
increased circulating triglyceride level / MGI
increased circulating free fatty acid level / MGI
postnatal growth retardation / MGI
increased colonic adenoma incidence / MGI
hepatic steatosis / MGI
decreased macrophage cell number / MGI
increased prostaglandin level / MGI
abnormal embryo development / MGI
abnormal embryo size / MGI
abnormal egg cylinder morphology / MGI
abnormal cell physiology / MGI
embryonic lethality, complete penetrance / MGI
decreased embryo size / MGI
embryonic lethality between implantation and placentation / MGI
absent mandible / MGI
abnormal brain development / MGI
acrania / MGI
perinatal lethality, complete penetrance / MGI
lethality throughout fetal growth and development, incomplete penetrance / MGI
absent midbrain / MGI
absent forebrain / MGI

MGI phenotypes (gene matching)

absent mandible / MGI
decreased hematocrit / MGI
increased cell proliferation / MGI
abnormal digestive system morphology / MGI
gastrointestinal hemorrhage / MGI
abnormal foregut morphology / MGI
abnormal crypts of Lieberkuhn morphology / MGI
rectal prolapse / MGI
abnormal intestinal mucosa morphology / MGI
abnormal mammary gland development / MGI
mammary gland hyperplasia / MGI
abnormal brain development / MGI
abnormal floor plate morphology / MGI
abnormal ovarian folliculogenesis / MGI
abnormal ovarian follicle morphology / MGI
absent mature ovarian follicles / MGI
absent corpus luteum / MGI
abnormal vagina epithelium morphology / MGI
dermal cysts / MGI
decreased body weight / MGI
decreased body size / MGI
increased metastatic potential / MGI
abnormal nursing / MGI
hunched posture / MGI
hyperlipidemia / MGI
increased circulating triglyceride level / MGI
increased circulating free fatty acid level / MGI
anemia / MGI
extended life span / MGI
abnormal embryo development / MGI
abnormal embryo size / MGI
decreased embryo size / MGI
incomplete embryo turning / MGI
postnatal growth retardation / MGI
increased mammary adenocarcinoma incidence / MGI
neoplasm / MGI
increased pilomatricoma incidence / MGI
increased tumor incidence / MGI
increased incidence of induced tumors / MGI
increased colon adenoma incidence / MGI
premature death / MGI
abnormal developmental patterning / MGI
abnormal embryonic tissue morphology / MGI
abnormal neural tube morphology / MGI
no abnormal phenotype detected / MGI
increased intestinal adenoma incidence / MGI
hepatic steatosis / MGI
increased trichoepithelioma incidence / MGI
increased intestinal adenocarcinoma incidence / MGI
no phenotypic analysis / MGI
abnormal egg cylinder morphology / MGI
acrania / MGI
colon polyps / MGI
intestinal obstruction / MGI
melena / MGI
rectal hemorrhage / MGI
gastric polyps / MGI
increased hepatocellular carcinoma incidence / MGI
epidermal cyst / MGI
decreased tumor growth/size / MGI
abnormal tumor morphology / MGI
abnormal intestinal goblet cell morphology / MGI
increased tumor growth/size / MGI
increased osteosarcoma incidence / MGI
decreased macrophage cell number / MGI
abnormal embryonic epiblast morphology / MGI
aneuploidy / MGI
abnormal primitive node morphology / MGI
cardia bifida / MGI
increased incidence of tumors by chemical induction / MGI
split notochord / MGI
ovary atrophy / MGI
abnormal large intestine crypts of Lieberkuhn morphology / MGI
uterus atrophy / MGI
decreased T cell number / MGI
increased circulating cholesterol level / MGI
cellular phenotype / MGI
reproductive system phenotype / MGI
abnormal cell physiology / MGI
increased apoptosis / MGI
abnormal hindbrain development / MGI
absent male preputial gland / MGI
intestine polyps / MGI
increased liver tumor incidence / MGI
decreased NK cell number / MGI
abnormal anterior visceral endoderm morphology / MGI
embryonic lethality / MGI
abnormal enterocyte proliferation / MGI
adrenal gland hyperplasia / MGI
abnormal mesendoderm development / MGI
increased small intestine adenocarcinoma incidence / MGI
increased large intestine adenocarcinoma incidence / MGI
decreased splenocyte number / MGI
abnormal pregnancy / MGI
absent nipple / MGI
increased prostaglandin level / MGI
embryonic lethality between implantation and placentation / MGI
increased gastrointestinal tumor incidence / MGI
increased mammary gland tumor incidence / MGI
increased skin tumor incidence / MGI
increased stomach tumor incidence / MGI
increased osteoma incidence / MGI
increased desmoid tumor incidence / MGI
gastrointestinal tract polyps / MGI
increased adenoma incidence / MGI
altered tumor pathology / MGI
mortality/aging / MGI
postnatal lethality, complete penetrance / MGI
postnatal lethality, incomplete penetrance / MGI
perinatal lethality, complete penetrance / MGI
prenatal lethality, complete penetrance / MGI
embryonic lethality, complete penetrance / MGI
embryonic lethality during organogenesis, complete penetrance / MGI
embryonic lethality between implantation and somite formation, incomplete penetrance / MGI
lethality throughout fetal growth and development, incomplete penetrance / MGI
absent midbrain / MGI
embryonic-extraembryonic boundary constriction / MGI
absent forebrain / MGI
rostral-caudal axis duplication / MGI
rostral body truncation / MGI
absent neural folds / MGI
abnormal head development / MGI
decreased intestinal adenoma incidence / MGI
abnormal endocardial heart tube morphology / MGI

Literature references

A dominant mutation that predisposes to multiple intestinal neoplasia in the mouse.;Moser A R, Pitot H C, Dove W F, ;1990;Science (New York, N.Y.);247;322-4; 2296722
Genetic basis of variation in adenoma multiplicity in ApcMin/+ Mom1S mice.;Haines Jackie, Johnson Victoria, Pack Kevin, Suraweera Nirosha, Slijepcevic Predrag, Cabuy Erik, Coster Margaret, Ilyas Mohammad, Wilding Jennifer, Sieber Oliver, Bodmer Walter, Tomlinson Ian, Silver Andrew, ;2005;Proceedings of the National Academy of Sciences of the United States of America;102;2868-73; 15710876

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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C57BL/6J-ApcMin/5H