IMPC phenotypes (gene matching)
B6;129P2-Gjc1tm1Weil/Cnrm
| Status | Available to order |
| EMMA ID | EM:01647 |
| Citation information | RRID:IMSR_EM:01647 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129P2-Gjc1tm1Weil/Cnrm |
| Alternative name | Cx45 flox |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Gjc1tm1Weil |
| Gene/Transgene symbol | Gjc1 |
Information from provider
| Provider | Klaus WILLECKE |
| Provider affiliation | Molekulargenetik, Institut fuer Genetik, Universitaet Bonn |
| Genetic information | Instead of the whole open reading frame of exon 3 of the Gjc1 (Cx45) gene, a loxP sites-flanked construct was inserted, containing the exon 3 of Gjc1. An frt-sites-flanked neomycin-selection cassette was placed in intron 2. After cre recombinase activity an eGFP reporter gene is expressed under the control of the endogenous Gjc1 promoter. |
| Phenotypic information | None (loxP-flanked gene mutation). |
| Breeding history | After blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown coat coloured offspring. This brown offspring was backcrossed to C57BL/6NCrl more than 3 times. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
| Breeding at archiving centre | Backcrossed to C57BL/6J |
Disease and phenotype information
MGI phenotypes (gene matching)
- abnormal heart development / MGI
- abnormal heart looping / MGI
- enlarged heart / MGI
- absent trabeculae carneae / MGI
- absent atrioventricular cushions / MGI
- impaired hematopoiesis / MGI
- decreased embryo size / MGI
- embryonic growth arrest / MGI
- thin myocardium / MGI
- no phenotypic analysis / MGI
- decreased cardiac output / MGI
- embryonic growth retardation / MGI
- small placenta / MGI
- decreased cardiac muscle contractility / MGI
- increased apoptosis / MGI
- heart block / MGI
- embryonic lethality during organogenesis, complete penetrance / MGI
- increased embryonic neuroepithelium apoptosis / MGI
Literature references
- Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission.;Maxeiner Stephan, Dedek Karin, Janssen-Bienhold Ulrike, Ammermüller Josef, Brune Hendrik, Kirsch Taryn, Pieper Mario, Degen Joachim, Krüger Olaf, Willecke Klaus, Weiler Reto, ;2005;The Journal of neuroscience : the official journal of the Society for Neuroscience;25;566-76; 15659592