B6;129P2-Gjc1tm1Weil/Cnrm
Status | Available to order |
EMMA ID | EM:01647 |
International strain name | B6;129P2-Gjc1tm1Weil/Cnrm |
Alternative name | Cx45 flox |
Strain type | Targeted Mutant Strains : Conditional mutation |
Allele/Transgene symbol | Gjc1tm1Weil |
Gene/Transgene symbol | Gjc1 |
Information from provider
Provider | Klaus WILLECKE |
Provider affiliation | Molekulargenetik, Institut fuer Genetik, Universitaet Bonn |
Genetic information | Instead of the whole open reading frame of exon 3 of the Gjc1 (Cx45) gene, a loxP sites-flanked construct was inserted, containing the exon 3 of Gjc1. An frt-sites-flanked neomycin-selection cassette was placed in intron 2. After cre recombinase activity an eGFP reporter gene is expressed under the control of the endogenous Gjc1 promoter. |
Phenotypic information | None (loxP-flanked gene mutation). |
Breeding history | After blastocyst injection the chimeras were bred with C57BL/6NCrl to obtain brown coat coloured offspring. This brown offspring was backcrossed to C57BL/6NCrl more than 3 times. |
References |
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Information from EMMA
Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Breeding at archiving centre | Backcrossed to C57BL/6J |
Disease and phenotype information
IMPC phenotypes (gene matching)
MGI phenotypes (gene matching)
- abnormal heart development / MGI
- abnormal heart looping / MGI
- enlarged heart / MGI
- absent trabeculae carneae / MGI
- absent atrioventricular cushions / MGI
- impaired hematopoiesis / MGI
- decreased embryo size / MGI
- embryonic growth arrest / MGI
- thin myocardium / MGI
- no phenotypic analysis / MGI
- decreased cardiac output / MGI
- embryonic growth retardation / MGI
- small placenta / MGI
- decreased cardiac muscle contractility / MGI
- increased apoptosis / MGI
- heart block / MGI
- embryonic lethality during organogenesis, complete penetrance / MGI
- increased embryonic neuroepithelium apoptosis / MGI
Literature references
- Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission.;Maxeiner Stephan, Dedek Karin, Janssen-Bienhold Ulrike, Ammermüller Josef, Brune Hendrik, Kirsch Taryn, Pieper Mario, Degen Joachim, Krüger Olaf, Willecke Klaus, Weiler Reto, ;2005;The Journal of neuroscience : the official journal of the Society for Neuroscience;25;566-76; 15659592
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