B6.Cg-Rag2^tm1Fwa Tyr^c Foxn1^nu/FwaOrl

Status	Available to order
EMMA ID	EM:00164
International strain name	B6.Cg-Rag2^tm1Fwa Tyr^c Foxn1^nu/FwaOrl
Alternative name	Rag2°-nu
Strain type	Spontaneous
Allele/Transgene symbol	Foxn1^nu, Rag2^tm1Fwa
Gene/Transgene symbol	Foxn1, Rag2

Information from provider

Provider	Frederick Alt
Provider affiliation	National Cancer Institute
Genetic information	B6-Rag2: mutant mice deficient in the Rag2 gene; obtained by mutation of the Rag2 gene by homologous recombination (insertion of the PMC1neo gene into the unique exon of Rag2 gene, leading to the deletion of 286 amino acids); nu (nude): forkhead box N1 nude mutation (Foxn1^nu).
Phenotypic information	Rag2 knock-out: homozygous mutants are viable even if they are smaller in size than wild-type or heterozygous littermates; homozygous animals do not produce mature B or T lymphocytes; deletion of a large portion of the Rag2 gene produces a total inability to initiate V(D)J rearrangement, leading to a severe combined immune deficiency (SCID). Foxn1^nu (nude): hairlessness, athymia and T-cell deficiency (immunodeficiency). We can provide only B6-Rag2 males and nu/+ females and the customer should have to do the breeding.
References	RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.;Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, ;1992;Cell;68;855-67; 1547487

Information from EMMA

Archiving centre	Institut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Severe combined immunodeficiency due to FOXN1 deficiency / Orphanet_169095
Severe combined immunodeficiency due to complete RAG1/2 deficiency / Orphanet_331206
Omenn syndrome / Orphanet_39041
Combined immunodeficiency with granulomatosis / Orphanet_157949

IMPC phenotypes (gene matching)

dystrophy / IMPC
preweaning lethality, incomplete penetrance / IMPC
small superior vagus ganglion / IMPC
abnormal thymus morphology / IMPC
athymia / IMPC
abnormal skin morphology / IMPC
hydrometra / IMPC

MGI phenotypes (allele matching)

small thymus / MGI
abnormal thymus morphology / MGI
increased incidence of corneal inflammation / MGI
increased susceptibility to viral infection / MGI
blepharitis / MGI
corneal vascularization / MGI
abnormal nail morphology / MGI
nail dystrophy / MGI
abnormal nail plate morphology / MGI
abnormal nail matrix morphology / MGI
decreased susceptibility to parasitic infection / MGI
decreased leukocyte cell number / MGI
abnormal liver morphology / MGI
abnormal spleen morphology / MGI
small spleen / MGI
athymia / MGI
small ovary / MGI
thin skin / MGI
decreased body weight / MGI
decreased body size / MGI
wavy vibrissae / MGI
short vibrissae / MGI
absent vibrissae / MGI
postnatal growth retardation / MGI
reduced male fertility / MGI
female infertility / MGI
abnormal estrous cycle / MGI
premature death / MGI
abnormal lymph node T cell domain morphology / MGI
abnormal Peyer's patch germinal center morphology / MGI
asthenozoospermia / MGI
hairless / MGI
abnormal venule morphology / MGI
abnormal splenic cell ratio / MGI
coiled sperm flagellum / MGI
postnatal lethality, incomplete penetrance / MGI
impaired spatial learning / MGI
increased bone mineral density / MGI
abnormal trabecular bone morphology / MGI
abnormal compact bone morphology / MGI
abnormal coat/ hair morphology / MGI
abnormal hair follicle morphology / MGI
deformed nails / MGI
abnormal thymus development / MGI
abnormal epidermal layer morphology / MGI
abnormal epidermis stratum basale morphology / MGI
abnormal epidermis stratum granulosum morphology / MGI
abnormal epidermis stratum corneum morphology / MGI
abnormal embryonic tissue morphology / MGI
abnormal hair shaft morphology / MGI
abnormal hair cuticle / MGI
abnormal hair cortex morphology / MGI
endocrine/exocrine gland phenotype / MGI
abnormal cutaneous collagen fibril morphology / MGI
reduced hair shaft melanin granule number / MGI
abnormal hair cortex keratinization / MGI
abnormal hair follicle inner root sheath morphology / MGI
short nails / MGI
decreased susceptibility to bacterial infection / MGI
decreased circulating alanine transaminase level / MGI
ascites / MGI
decreased circulating tumor necrosis factor level / MGI
decreased interferon-gamma secretion / MGI
decreased circulating interleukin-6 level / MGI
decreased circulating interleukin-2 level / MGI
chromosomal instability / MGI
increased susceptibility to viral infection induced morbidity/mortality / MGI
abnormal circulating cytokine level / MGI
increased plasmacytoma incidence / MGI
decreased circulating luteinizing hormone level / MGI
decreased circulating follicle stimulating hormone level / MGI
abnormal circulating gonadotropin level / MGI
abnormal T cell activation / MGI
abnormal lymph node morphology / MGI
increased length of allograft survival / MGI
decreased lymphocyte cell number / MGI
decreased T cell number / MGI
abnormal spleen physiology / MGI
abnormal muscle morphology / MGI
decreased skeletal muscle mass / MGI
decreased skeletal muscle fiber size / MGI
increased granulocyte number / MGI
spleen hypoplasia / MGI
arrested B cell differentiation / MGI
decreased IgM level / MGI
thymus hypoplasia / MGI
arrested T cell differentiation / MGI
increased susceptibility to infection / MGI
abnormal effector T cell morphology / MGI
increased natural killer cell mediated cytotoxicity / MGI
decreased double-positive T cell number / MGI
increased macrophage cell number / MGI
decreased CD4-positive, alpha beta T cell number / MGI
decreased CD8-positive, alpha-beta T cell number / MGI
lymph node hypoplasia / MGI
absent mature B cells / MGI
abnormal T cell receptor V(D)J recombination / MGI
abnormal immunoglobulin V(D)J recombination / MGI
decreased thymocyte number / MGI
lung inflammation / MGI
increased susceptibility to bacterial infection / MGI
abnormal macrophage physiology / MGI
colitis / MGI
abnormal T-helper 1 physiology / MGI
abnormal response to transplant / MGI
abnormal interferon secretion / MGI
abnormal chemokine secretion / MGI
decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
abnormal intestinal mucosa morphology / MGI
abnormal B cell morphology / MGI
decreased double-negative T cell number / MGI
increased double-negative T cell number / MGI
decreased susceptibility to type IV hypersensitivity reaction / MGI
abnormal stomach mucosa morphology / MGI
abnormal intestinal epithelium morphology / MGI
abnormal B cell number / MGI
decreased pre-B cell number / MGI
decreased mature B cell number / MGI
increased immature B cell number / MGI
decreased immature B cell number / MGI
abnormal gamma-delta T cell differentiation / MGI

MGI phenotypes (gene matching)

increased bone mineral density / MGI
abnormal trabecular bone morphology / MGI
abnormal leukocyte cell number / MGI
increased neutrophil cell number / MGI
decreased leukocyte cell number / MGI
increased granulocyte number / MGI
abnormal coat/ hair morphology / MGI
abnormal hair follicle morphology / MGI
underdeveloped hair follicles / MGI
abnormal nail morphology / MGI
deformed nails / MGI
abnormal liver morphology / MGI
abnormal immune system morphology / MGI
abnormal spleen morphology / MGI
small spleen / MGI
spleen hypoplasia / MGI
abnormal Peyer's patch morphology / MGI
abnormal thymus morphology / MGI
abnormal thymus development / MGI
athymia / MGI
small thymus / MGI
thymus hyperplasia / MGI
decreased thymocyte number / MGI
abnormal immune system cell morphology / MGI
abnormal lymphocyte cell number / MGI
small ovary / MGI
thin skin / MGI
wrinkled skin / MGI
abnormal epidermal layer morphology / MGI
abnormal epidermis stratum basale morphology / MGI
abnormal epidermis stratum granulosum morphology / MGI
abnormal epidermis stratum corneum morphology / MGI
decreased body weight / MGI
decreased body size / MGI
curly vibrissae / MGI
wavy vibrissae / MGI
short vibrissae / MGI
sparse vibrissae / MGI
absent vibrissae / MGI
increased incidence of corneal inflammation / MGI
postnatal growth retardation / MGI
abnormal immune system physiology / MGI
abnormal humoral immune response / MGI
thymus hypoplasia / MGI
abnormal T cell activation / MGI
reduced male fertility / MGI
reduced female fertility / MGI
female infertility / MGI
abnormal estrous cycle / MGI
decreased litter size / MGI
increased sarcoma incidence / MGI
abnormal hair growth / MGI
premature death / MGI
abnormal embryonic tissue morphology / MGI
abnormal skin pigmentation / MGI
abnormal vibrissa morphology / MGI
abnormal muscle morphology / MGI
abnormal T cell differentiation / MGI
no abnormal phenotype detected / MGI
abnormal lymph node morphology / MGI
abnormal lymph node T cell domain morphology / MGI
abnormal Peyer's patch germinal center morphology / MGI
decreased susceptibility to bacterial infection / MGI
increased susceptibility to viral infection / MGI
abnormal CD4-positive, alpha beta T cell morphology / MGI
decreased immunoglobulin level / MGI
asthenozoospermia / MGI
abnormal immune system organ morphology / MGI
decreased circulating luteinizing hormone level / MGI
decreased circulating follicle stimulating hormone level / MGI
decreased circulating alanine transaminase level / MGI
abnormal circulating gonadotropin level / MGI
abnormal hair follicle development / MGI
abnormal compact bone morphology / MGI
abnormal hair shaft morphology / MGI
abnormal hair cuticle / MGI
abnormal hair cortex morphology / MGI
hairless / MGI
brittle hair / MGI
abnormal venule morphology / MGI
decreased incidence of tumors by chemical induction / MGI
increased length of allograft survival / MGI
decreased skeletal muscle mass / MGI
abnormal CD8-positive, alpha beta T cell morphology / MGI
increased eosinophil cell number / MGI
increased B cell number / MGI
decreased lymphocyte cell number / MGI
decreased T cell number / MGI
decreased susceptibility to parasitic infection / MGI
decreased double-negative T cell number / MGI
increased double-positive T cell number / MGI
decreased double-positive T cell number / MGI
cachexia / MGI
blepharitis / MGI
ascites / MGI
endocrine/exocrine gland phenotype / MGI
immune system phenotype / MGI
corneal vascularization / MGI
absent T cells / MGI
decreased CD4-positive, alpha beta T cell number / MGI
decreased single-positive T cell number / MGI
lymph node hypoplasia / MGI
abnormal cutaneous collagen fibril morphology / MGI
decreased circulating tumor necrosis factor level / MGI
decreased interferon-gamma secretion / MGI
decreased circulating interleukin-6 level / MGI
decreased circulating interleukin-2 level / MGI
reduced hair shaft melanin granule number / MGI
abnormal spleen physiology / MGI
abnormal splenic cell ratio / MGI
chromosomal instability / MGI
coiled sperm flagellum / MGI
decreased skeletal muscle fiber size / MGI
abnormal thymus epithelium morphology / MGI
increased susceptibility to viral infection induced morbidity/mortality / MGI
abnormal hair cortex keratinization / MGI
increased DN1 thymic pro-T cell number / MGI
abnormal circulating cytokine level / MGI
absent thymus cortex / MGI
decreased lymphoma incidence / MGI
increased plasmacytoma incidence / MGI
abnormal hair follicle bulb morphology / MGI
abnormal hair follicle inner root sheath morphology / MGI
integument phenotype / MGI
postnatal lethality, incomplete penetrance / MGI
increased or absent threshold for auditory brainstem response / MGI
impaired spatial learning / MGI
short nails / MGI
nail dystrophy / MGI
abnormal nail plate morphology / MGI
abnormal nail matrix morphology / MGI
absent thymus medulla / MGI
thymus cysts / MGI
abnormal corneocyte morphology / MGI
decreased monocyte cell number / MGI
increased granulocyte number / MGI
decreased bone marrow cell number / MGI
alopecia / MGI
abnormal digestive system morphology / MGI
abnormal intestinal epithelium morphology / MGI
abnormal intestinal mucosa morphology / MGI
abnormal spleen morphology / MGI
small spleen / MGI
spleen hypoplasia / MGI
abnormal Peyer's patch morphology / MGI
abnormal thymus morphology / MGI
decreased thymocyte number / MGI
abnormal immune system cell morphology / MGI
decreased body size / MGI
abnormal humoral immune response / MGI
arrested B cell differentiation / MGI
decreased IgG level / MGI
decreased IgM level / MGI
decreased IgA level / MGI
thymus hypoplasia / MGI
arrested T cell differentiation / MGI
lung inflammation / MGI
neoplasm / MGI
abnormal B cell differentiation / MGI
no abnormal phenotype detected / MGI
small lymph nodes / MGI
abnormal lymph node morphology / MGI
abnormal lymphopoiesis / MGI
abnormal pre-B cell morphology / MGI
increased susceptibility to infection / MGI
abnormal double-negative T cell morphology / MGI
abnormal double-positive T cell morphology / MGI
increased susceptibility to bacterial infection / MGI
abnormal effector T cell morphology / MGI
abnormal macrophage physiology / MGI
abnormal B cell number / MGI
abnormal B cell physiology / MGI
abnormal immune system organ morphology / MGI
colitis / MGI
absent Peyer's patches / MGI
increased natural killer cell mediated cytotoxicity / MGI
erythroderma / MGI
abnormal lymphocyte physiology / MGI
abnormal B cell morphology / MGI
decreased lymphocyte cell number / MGI
decreased B cell number / MGI
decreased T cell number / MGI
decreased susceptibility to parasitic infection / MGI
decreased double-negative T cell number / MGI
increased double-negative T cell number / MGI
decreased double-positive T cell number / MGI
decreased T cell proliferation / MGI
cachexia / MGI
increased macrophage cell number / MGI
abnormal T-helper 1 physiology / MGI
decreased susceptibility to type IV hypersensitivity reaction / MGI
abnormal response to transplant / MGI
abnormal T cell morphology / MGI
decreased NK T cell number / MGI
decreased CD4-positive, alpha beta T cell number / MGI
decreased CD8-positive, alpha-beta T cell number / MGI
lymph node hypoplasia / MGI
decreased pre-B cell number / MGI
decreased mature B cell number / MGI
absent mature B cells / MGI
increased immature B cell number / MGI
decreased immature B cell number / MGI
abnormal gamma-delta T cell differentiation / MGI
decreased spleen white pulp amount / MGI
abnormal interferon secretion / MGI
abnormal chemokine secretion / MGI
abnormal T cell receptor V(D)J recombination / MGI
abnormal immunoglobulin V(D)J recombination / MGI
absent Hassall's corpuscle / MGI
small inguinal lymph nodes / MGI
decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
increased DN3 thymocyte number / MGI
abnormal stomach mucosa morphology / MGI
small cervical lymph nodes / MGI

Literature references

RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.;Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, ;1992;Cell;68;855-67; 1547487

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6.Cg-Rag2tm1Fwa Tyrc Foxn1nu/FwaOrl