STOCK Rag2tm1Fwa/Orl

Status

Available to order

EMMA IDEM:00161
International strain nameSTOCK Rag2tm1Fwa/Orl
Alternative nameRag2°
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolRag2tm1Fwa
Gene/Transgene symbolRag2

Information from provider

ProviderFrederick Alt
Provider affiliationNational Cancer Institute
Genetic informationMutant mice deficient in Rag2 gene. Obtained by mutation of the Rag2 gene by homologous recombination (insertion of the PMC1neo gene into the unique exon of Rag2 gene, leading to the deletion of 286 amino acids).
Phenotypic informationHomozygous mutants are viable even if they are smaller in size than wild-type or heterozygous littermates. Homozygous animals do not produce mature B or T lymphocytes. Deletion of a large portion of the Rag2 gene produces a total inability to initiate V(D)J rearrangement, leading to a severe combined immune deficiency (SCID).
References
  • RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.;Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, ;1992;Cell;68;855-67; 1547487

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous 129S2/SvPas, heterozygous 129S2/SvPas
Stage of embryos2-cell

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • increased granulocyte number / MGI
  • abnormal spleen morphology / MGI
  • spleen hypoplasia / MGI
  • decreased body size / MGI
  • arrested B cell differentiation / MGI
  • decreased IgM level / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • increased susceptibility to infection / MGI
  • abnormal effector T cell morphology / MGI
  • increased natural killer cell mediated cytotoxicity / MGI
  • decreased double-positive T cell number / MGI
  • increased macrophage cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • lymph node hypoplasia / MGI
  • absent mature B cells / MGI
  • abnormal T cell receptor V(D)J recombination / MGI
  • abnormal immunoglobulin V(D)J recombination / MGI
  • decreased thymocyte number / MGI
  • lung inflammation / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal macrophage physiology / MGI
  • colitis / MGI
  • abnormal T-helper 1 physiology / MGI
  • abnormal response to transplant / MGI
  • abnormal interferon secretion / MGI
  • abnormal chemokine secretion / MGI
  • decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
  • abnormal intestinal mucosa morphology / MGI
  • abnormal B cell morphology / MGI
  • decreased susceptibility to parasitic infection / MGI
  • decreased double-negative T cell number / MGI
  • increased double-negative T cell number / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • abnormal stomach mucosa morphology / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal B cell number / MGI
  • decreased pre-B cell number / MGI
  • decreased mature B cell number / MGI
  • increased immature B cell number / MGI
  • decreased immature B cell number / MGI
  • abnormal gamma-delta T cell differentiation / MGI
MGI phenotypes (gene matching)
  • decreased monocyte cell number / MGI
  • increased granulocyte number / MGI
  • decreased bone marrow cell number / MGI
  • alopecia / MGI
  • abnormal digestive system morphology / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal intestinal mucosa morphology / MGI
  • abnormal spleen morphology / MGI
  • small spleen / MGI
  • spleen hypoplasia / MGI
  • abnormal Peyer's patch morphology / MGI
  • abnormal thymus morphology / MGI
  • decreased thymocyte number / MGI
  • abnormal immune system cell morphology / MGI
  • decreased body size / MGI
  • abnormal humoral immune response / MGI
  • arrested B cell differentiation / MGI
  • decreased IgG level / MGI
  • decreased IgM level / MGI
  • decreased IgA level / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • lung inflammation / MGI
  • neoplasm / MGI
  • abnormal B cell differentiation / MGI
  • no abnormal phenotype detected / MGI
  • small lymph nodes / MGI
  • abnormal lymph node morphology / MGI
  • abnormal lymphopoiesis / MGI
  • abnormal pre-B cell morphology / MGI
  • increased susceptibility to infection / MGI
  • abnormal double-negative T cell morphology / MGI
  • abnormal double-positive T cell morphology / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal effector T cell morphology / MGI
  • abnormal macrophage physiology / MGI
  • abnormal B cell number / MGI
  • abnormal B cell physiology / MGI
  • abnormal immune system organ morphology / MGI
  • colitis / MGI
  • absent Peyer's patches / MGI
  • increased natural killer cell mediated cytotoxicity / MGI
  • erythroderma / MGI
  • abnormal lymphocyte physiology / MGI
  • abnormal B cell morphology / MGI
  • decreased lymphocyte cell number / MGI
  • decreased B cell number / MGI
  • decreased T cell number / MGI
  • decreased susceptibility to parasitic infection / MGI
  • decreased double-negative T cell number / MGI
  • increased double-negative T cell number / MGI
  • decreased double-positive T cell number / MGI
  • decreased T cell proliferation / MGI
  • cachexia / MGI
  • increased macrophage cell number / MGI
  • abnormal T-helper 1 physiology / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • abnormal response to transplant / MGI
  • abnormal T cell morphology / MGI
  • decreased NK T cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • lymph node hypoplasia / MGI
  • decreased pre-B cell number / MGI
  • decreased mature B cell number / MGI
  • absent mature B cells / MGI
  • increased immature B cell number / MGI
  • decreased immature B cell number / MGI
  • abnormal gamma-delta T cell differentiation / MGI
  • decreased spleen white pulp amount / MGI
  • abnormal interferon secretion / MGI
  • abnormal chemokine secretion / MGI
  • abnormal T cell receptor V(D)J recombination / MGI
  • abnormal immunoglobulin V(D)J recombination / MGI
  • absent Hassall's corpuscle / MGI
  • small inguinal lymph nodes / MGI
  • decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
  • increased DN3 thymocyte number / MGI
  • abnormal stomach mucosa morphology / MGI
  • small cervical lymph nodes / MGI

Literature references

  • RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.;Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, ;1992;Cell;68;855-67; 1547487

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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