B6N.129S7-Defb41tm1(icre)Psip/Oulu
| Status | Available to order |
| EMMA ID | EM:15669 |
| Citation information | RRID:IMSR_EM:15669 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6N.129S7-Defb41tm1(icre)Psip/Oulu |
| Alternative name | Defb41tm1(iCre) |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Defb41tm1(icre)Psip |
| Gene/Transgene symbol | Defb41 |
Information from provider
| Provider | Petra Sipilä |
| Provider affiliation | University of Turku |
| Genetic information | icre-neomycin phosphotransferase (neo) expression cassette was inserted into the translation initiation site of the Defb41 gene in AB2.2 embryonic stem (ES) cells (129/SvEv background). Correctly targeted ES cells were injected into C57BL/6N blastocysts and chimeric males were bred to wild type C57BL/6N females. icre is expressed under the control of the regulatory region of Defb41. |
| Phenotypic information | Homozygous:As homozygous these animals can be used to study the lack of defensin beta 41 gene. Homozygous male mice are sub-fertile as sperm maturation in the epididymis is affected.Heterozygous:As heterozygous, these animals can be used as cre-expressing mouse line specifically in the principal cells of the epididymal epithelium in crosses with mice carrying floxed genes. Het mice do not have any harmful phenotype. |
| Breeding history | Mouse line has been bred into C57BL/6N background for more than 10 generations. |
| References |
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| Homozygous fertile | females only |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Oulu, Oulu, Finland |
Literature references
- Targeted inactivation of the mouse epididymal beta-defensin 41 alters sperm flagellar beat pattern and zona pellucida binding.;Björkgren Ida, Alvarez Luis, Blank Nelli, Balbach Melanie, Turunen Heikki, Laajala Teemu Daniel, Toivanen Jussi, Krutskikh Anton, Wahlberg Niklas, Huhtaniemi Ilpo, Poutanen Matti, Wachten Dagmar, Sipilä Petra, ;2016;Molecular and cellular endocrinology;427;143-54; 26987518