B6J.B6N(Cg)-Nos1tm1.1(cre/ERT2)Geno/RjIeg
| Status | Available to order |
| EMMA ID | EM:15641 |
| International strain name | B6J.B6N(Cg)-Nos1tm1.1(cre/ERT2)Geno/RjIeg |
| Alternative name | B6.Cg-Nos1tm1(cre/ERT2)Geno/JRj |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Nos1tm1.1(cre/ERT2)Geno |
| Gene/Transgene symbol | Nos1 |
Information from provider
| Provider | Alexandre Fraichard |
| Provider affiliation | genOway |
| Genetic information | A targeting vector was designed to insert IRES.Cre-ERT2 in frame into exon 33 of the neuronal nitric oxide (Nos1) gene, followed by a frt-flanked neomycin resistance cassette. The construct was electroporated into C57BL/6N-derived embryonic stem cells. Correctly targeted embryonic stem cells were injected into BALB/c blastocysts and resulting chimeric males were bred to C57BL/6J females. Offspring was bred with C57BL/6 deleter mice to excise the neo cassette and to generate heterozygous mice carrying the knock-in allele. |
| Phenotypic information | Homozygous:No phenotypic aberrations recordedHeterozygous:No phenotypic aberrations recorded |
| Breeding history | Mice have been backcrossed to C57BL/6JRj mice for 17 generations |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6J |