B6J.B6N(Cg)-Nos1tm1.1(cre/ERT2)Geno/RjIeg
| Status | Available to order |
| EMMA ID | EM:15641 |
| Citation information | RRID:IMSR_EM:15641 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6J.B6N(Cg)-Nos1tm1.1(cre/ERT2)Geno/RjIeg |
| Alternative name | B6.Cg-Nos1tm1(cre/ERT2)Geno/JRj |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Nos1tm1.1(cre/ERT2)Geno |
| Gene/Transgene symbol | Nos1 |
Information from provider
| Provider | Alexandre Fraichard |
| Provider affiliation | genOway |
| Genetic information | A targeting vector was designed to insert IRES.Cre-ERT2 in frame into exon 33 of the neuronal nitric oxide (Nos1) gene, followed by a frt-flanked neomycin resistance cassette. The construct was electroporated into C57BL/6N-derived embryonic stem cells. Correctly targeted embryonic stem cells were injected into BALB/c blastocysts and resulting chimeric males were bred to C57BL/6J females. Offspring was bred with C57BL/6 deleter mice to excise the neo cassette and to generate heterozygous mice carrying the knock-in allele. |
| Phenotypic information | Homozygous:No phenotypic aberrations recordedHeterozygous:No phenotypic aberrations recorded |
| Breeding history | Mice have been backcrossed to C57BL/6JRj mice for 17 generations |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | heterozygous C57BL/6J males |