C57BL/6N-Kif5ctm1.2Ics/Ics
| Status | Available to order |
| EMMA ID | EM:15621 |
| International strain name | C57BL/6N-Kif5ctm1.2Ics/Ics |
| Alternative name | Kif5ctm1.2Ics (G4563) |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Kif5ctm1.2Ics |
| Gene/Transgene symbol | Kif5c |
Information from provider
| Provider | ICS, Institut Clinique de la Souris |
| Provider affiliation | ICS, Institut Clinique de la Souris |
| Genetic information | This line was obtained by modification of BD10 in house derived C57BL/6N embryonic stem cells.The A to T mutation (codon GAA to GTA) leading to the E237V point mutation has been inserted in exon 8 (ENSMUSE00000162501) and allows the expression of the mutated protein. The same exon 8 was also floxed so that the excision of this critical exon will lead to a knock-out after cre recombinase-mediated excision in between the two LoxP sites. The flipped NeoR cassette was removed by breeding the male chimeras with flp recombinase deleter females. The knock-out allele was obtained after an additional breeding of the conditional allele with a cre recombinase deleter line. The line was generated on a pure C57BL/6N inbred genetic background. For detailed information on the genetic description of this strain, please have a look at this report. |
| Phenotypic information | Homozygous:Homozygous mice are subviableHeterozygous:DOI: 10.3390/biomedicines10123148 |
| Breeding history | Inbred C57BL/6N |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France |
Literature references
- Large-Scale Functional Assessment of Genes Involved in Rare Diseases with Intellectual Disabilities Unravels Unique Developmental and Behaviour Profiles in Mouse Models.;Meziane Hamid, Birling Marie-Christine, Wendling Olivia, Leblanc Sophie, Dubos Aline, Selloum Mohammed, Pavlovic Guillaume, Sorg Tania, Kalscheuer Vera M, Billuart Pierre, Laumonnier Frédéric, Chelly Jamel, van Bokhoven Hans, Herault Yann, ;2022;Biomedicines;10;; 36551904