C57BL/6N-Tg(Nr2f2-cre)40Ics/Ics

Status

Available to order

EMMA IDEM:15559
International strain nameC57BL/6N-Tg(Nr2f2-cre)40Ics/Ics
Alternative nameTg(Nr2f2-cre)40.ICS
Strain typeTransgenic Strains
Allele/Transgene symbolTg(Nr2f2-cre)40.ICS
Gene/Transgene symbolTg(Nr2f2-cre)40.ICS

Information from provider

Provider ICS, Institut Clinique de la Souris
Provider affiliationICS, Institut Clinique de la Souris
Genetic informationThis line was obtained by crossing Tg(Nr2f2-cre/ERT2)40.ICS animals with the C57BL/6NTac-Gt(ROSA)26Sor (EMMA strain ID EM:05490) ubiquitous flp deleter resulting in a cre-F3 sequence located at the ATG of the Nrf2f2 gene followed by a roxed PGK-Tn5-Kana/NeoR cassette. The pure C57BL/6N background was maintained for all breeding steps. Efficiency of deletion was confirmed by crossing this line with the cre recombinase-inducible fluorescent reporter Gt(ROSA)26Sor. EGFP labeling was observed in a structure next to the ovary which could be the oviduct/uterus transition, no labeling in the oviduct was observed. In the heart, EGFP labelling is observed in some cardiomyocytes. In uterus, EGFP labelling is observed in smooth muscle cells. In bladder, EGFP labelling is observed in few smooth muscle cells. For detailed information on the genetic description of this strain, please have a look at this report.
Phenotypic informationHomozygous:
NA

Heterozygous:
NA
Breeding historyInbred to C57BL/6N for 3 generations.
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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