C57BL/6N-Prg2tm1.2(RFP657/cre)Ics/Ics

Status

Available to order

EMMA IDEM:15556
International strain nameC57BL/6N-Prg2tm1.2(RFP657/cre)Ics/Ics
Alternative namePrg2tm1.2(RFP657/cre)Ics
Strain typeEndonuclease-mediated
Allele/Transgene symbolPrg2tm1.2(RFP657/cre)Ics
Gene/Transgene symbolPrg2

Information from provider

Provider ICS, Institut Clinique de la Souris
Provider affiliationICS, Institut Clinique de la Souris
Genetic informationThis line was obtained by crossing Prg2tm1.1(RFP657/cre/ERT2)Ics animals with the C57BL/6NTac-Gt(ROSA)26Sortm2(CAG-flpo,-EYFP)Ics flp ubiquitous deleter (EMMA strain ID EM:05490) resulting in a En2-RFP657-LF2A-cre-F3 sequence located between exon 2 and exon 3 in the intronic region of Prg2. The pure C57BL/6N background was maintained for all breeding steps. FACS analysis showed efficient and specific cre expression in spleen eosinophils. TagRFP657 is not detected due to the mutation in its cDNA. For detailed information on the genetic description of this strain, please have a look at this report.
Phenotypic informationHomozygous:
NA

Heterozygous:
NA
Breeding historyC57BL/6N inbred (4 generations).
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France

Disease and phenotype information

IMPC phenotypes (gene matching)
  • abnormal coat appearance / IMPC
  • increased respiratory quotient / IMPC
  • abnormal eye posterior chamber depth / IMPC

Information on how we integrate external resources can be found here

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable), as well as a CRISPR surcharge.

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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