IMPC phenotypes (gene matching)
C57BL/6N-Prg2tm1.1(RFP657/cre/ERT2)Ics/Ics
| Status | Available to order |
| EMMA ID | EM:15554 |
| International strain name | C57BL/6N-Prg2tm1.1(RFP657/cre/ERT2)Ics/Ics |
| Alternative name | Prg2tm1.1(RFP657/cre/ERT2)Ics |
| Strain type | Endonuclease-mediated |
| Allele/Transgene symbol | Prg2tm1.1(RFP657/cre/ERT2)Ics |
| Gene/Transgene symbol | Prg2 |
Information from provider
| Provider | ICS, Institut Clinique de la Souris |
| Provider affiliation | ICS, Institut Clinique de la Souris |
| Genetic information | This line was obtained by modification of S3 C57BL/6N embryonic stem cells. A 5.6 kb trap cassette was inserted by homologous recombination in ES cells in the intronic region of Prg2 located between exon 2 and exon 3. This cassette contains intron 1 and partial exon sequence of En2 to act as splice donor site, a LF2A (2A self-cleaving peptide) sequence that can induce ribosomal skipping during translation, a mutated TagRFP657 far-red monomeric fluorescent protein sequence, a T2A sequence and a dual cre-F3-ERT2-F3 cassette which allow expression of creERT2 or cre (if crossed with a flp deleter), and a selection cassette. The pPGK-Neo selection cassette flanked by rox attachment sites was deleted in vivo by crossing with a C57BL/6N dre ubiquitous deleter. FACS analysis showed efficient and specific creERT2 expression in spleen eosinophils. TagRFP657 is not detected due to the mutation in its cDNA. For detailed information on the genetic description of this strain, please have a look at this report. |
| Phenotypic information | Homozygous:NAHeterozygous:NA |
| Breeding history | C57BL/6N inbred (3 generations). |
| References | None available |
| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France |