C57BL/6N-Mcpt8tm1.1(cre)Ics/Ics
| Status | Available to order |
| EMMA ID | EM:15553 |
| International strain name | C57BL/6N-Mcpt8tm1.1(cre)Ics/Ics |
| Alternative name | Mcpt8tm1.1(Cre/TdTomato)Ics |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Mcpt8tm1.1(cre)Ics |
| Gene/Transgene symbol | Mcpt8 |
Information from provider
| Provider | Nicolas Charles |
| Provider affiliation | Centre de Recherche sur l, Université Paris Cité |
| Genetic information | This line was obtained by modification of S3 C57BL/6N embryonic stem cells. A Cre-T2A-TdTomato cassette was inserted after the STOP codon of Mcpt8, by homologous recombination in ES cells. The pPGK-Neo selection cassette flanked by FRT sites was removed by breeding chimeras with a C57BL/6N flp recombinase deleter line. This line has been published, with description of cre recombinase and Td-Tomato specific expression in basophils. A detailed technical report of model generation is also enclosed. For detailed information on the genetic description of this strain, please see this report. |
| Phenotypic information | Homozygous:NAHeterozygous:NA |
| Breeding history | Inbred to C57BL/6N for 2 generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | ICS, Institut Clinique de la Souris, Illkirch-Graffenstaden, France |
Literature references
- CT-M8 Mice: A New Mouse Model Demonstrates That Basophils Have a Nonredundant Role in Lupus-Like Disease Development.;Tchen John, Simon Quentin, Chapart Léa, Pellefigues Christophe, Karasuyama Hajime, Miyake Kensuke, Blank Ulrich, Benhamou Marc, Daugas Eric, Charles Nicolas, ;2022;Frontiers in immunology;13;900532; 35844602