IMPC phenotypes (allele matching)
B6.CAST-Gpx1tm1.1(KOMP)Vlcg Cdh23Ahl+/Ieg
| Status | Available to order |
| EMMA ID | EM:15539 |
| International strain name | B6.CAST-Gpx1tm1.1(KOMP)Vlcg Cdh23Ahl+/Ieg |
| Alternative name | C57Bl/6.CAST-Cdh23Ahl+-Gpx1KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Gpx1tm1.1(KOMP)Vlcg, Cdh23Ahl+ |
| Gene/Transgene symbol | Gpx1, Cdh23 |
Information from provider
| Provider | Georg Klump |
| Provider affiliation | Department of Neuroscience, Carl von Ossietzky University Oldenburg |
| Additional owner | Dr. Marten Szibor, BioMediTech & Tampere University Hospital, Faculty of Medicine and Health Technology, FI-33014 Tampere University, Finland. |
| Genetic information | The gene coding the cellular glutathione peroxidase 1 (Gpx1) has been silenced by KOMP as described elsewhere (https://www.mmrrc.org/catalog/sds.php?mmrrc_id=47982). ES cells were propagated and injected into blastocyst stage embryos in the laboratory of Prof. Howard T. Jacobs at the University of Helsinki. Mice were then backcrossed to the C57BL/6.CAST-Cdh23Ahl+ background in the laboratory of Prof. Georg Klump at the University of Oldenburg. This strain is only available as frozen sperm. It is recommended to use B6.CAST-Cdh23Ahl+/Kjn (available from The Jackson Laboratory) as oocyte donors for rederivation and afterwards produce homozygous Gpx1 knock-out mice by intercrossing. In case the rederivation is done in the C57BL/6J background, the recipient will need to produce homozygous mice (i.e. homzoygous for Gpx1 knock-out and for B6.CAST-Cdh23Ahl+ background), by appropriate crossing within the F1 progeny. |
| Phenotypic information | Homozygous:No obvious phenotype. A previous study (Ohlemiller et al., 2000; DOI 10.1007/s101620010043) suggests increased susceptibility to noise-induced hearing loss when knocked-out in mice with C57BL/6 background.Heterozygous:No obvious phenotype. |
| Breeding history | A minimum of eight generations of half- and/or sib mating on C57BL/6.CAST-Cdh23Ahl+ background have been performed with Gpx1 knock-out mice. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
| Animals used for archiving | homozygous 0 |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Usher syndrome type 1 / Orphanet_231169
- Autosomal recessive non-syndromic sensorineural deafness type DFNB / Orphanet_90636
IMPC phenotypes (gene matching)
- decreased circulating calcium level / IMPC
- preweaning lethality, incomplete penetrance / IMPC
- absent pinna reflex / IMPC
- small testis / IMPC
- decreased body length / IMPC
- abnormal testis morphology / IMPC
- decreased circulating glucose level / IMPC
- increased mean corpuscular hemoglobin / IMPC
- trunk curl / IMPC
- increased lean body mass / IMPC
- decreased total body fat amount / IMPC
- increased circulating sodium level / IMPC
- abnormal gait / IMPC
- impaired righting response / IMPC
- decreased fasting circulating glucose level / IMPC
- increased circulating chloride level / IMPC
- stereotypic behavior / IMPC
- abnormal epididymis morphology / IMPC
- decreased leukocyte cell number / IMPC
- increased bone mineral content / IMPC
- enlarged epididymis / IMPC
- decreased body length / IMPC
MGI phenotypes (gene matching)
- abnormal ear position / MGI
- abnormal inner ear morphology / MGI
- abnormal cochlea morphology / MGI
- abnormal organ of Corti morphology / MGI
- organ of Corti degeneration / MGI
- abnormal stria vascularis morphology / MGI
- tremors / MGI
- abnormal maternal nurturing / MGI
- abnormal stationary movement / MGI
- abnormal locomotor behavior / MGI
- ataxia / MGI
- circling / MGI
- bidirectional circling / MGI
- hyperactivity / MGI
- abnormal gait / MGI
- head bobbing / MGI
- decreased grooming behavior / MGI
- abnormal startle reflex / MGI
- decreased startle reflex / MGI
- impaired swimming / MGI
- decreased litter size / MGI
- abnormal reflex / MGI
- abnormal hearing physiology / MGI
- deafness / MGI
- abnormal motor capabilities/coordination/movement / MGI
- abnormal ear morphology / MGI
- abnormal cochlear hair cell morphology / MGI
- head shaking / MGI
- decreased vertical activity / MGI
- abnormal cochlear ganglion morphology / MGI
- cochlear ganglion degeneration / MGI
- abnormal otolith morphology / MGI
- abnormal response to novelty / MGI
- abnormal cochlear sensory epithelium morphology / MGI
- cochlear ganglion hypoplasia / MGI
- abnormal defecation / MGI
- abnormal ear physiology / MGI
- vestibular saccular degeneration / MGI
- vestibular saccular macula degeneration / MGI
- cochlear hair cell degeneration / MGI
- stria vascularis degeneration / MGI
- decreased cochlear inner hair cell number / MGI
- cochlear inner hair cell degeneration / MGI
- abnormal cochlear outer hair cell morphology / MGI
- decreased cochlear outer hair cell number / MGI
- cochlear outer hair cell degeneration / MGI
- decreased cochlear hair cell number / MGI
- abnormal cochlear nerve compound action potential / MGI
- abnormal hair cell mechanoelectric transduction / MGI
- abnormal cochlear hair cell physiology / MGI
- abnormal cochlear outer hair cell physiology / MGI
- abnormal orientation of outer hair cell stereociliary bundles / MGI
- abnormal orientation of inner hair cell stereociliary bundles / MGI
- abnormal vestibular hair cell stereociliary bundle morphology / MGI
- abnormal cochlear hair cell stereociliary bundle morphology / MGI
- abnormal orientation of cochlear hair cell stereociliary bundles / MGI
- decreased cochlear hair cell stereocilia number / MGI
- abnormal outer hair cell stereociliary bundle morphology / MGI
- decreased outer hair cell stereocilia number / MGI
- absent outer hair cell stereocilia / MGI
- abnormal inner hair cell stereociliary bundle morphology / MGI
- abnormal cochlear hair cell inter-stereocilial links morphology / MGI
- abnormal cochlear hair bundle tip links morphology / MGI
- increased susceptibility to noise-induced hearing loss / MGI
- abnormal distortion product otoacoustic emission / MGI
- absent distortion product otoacoustic emissions / MGI
- abnormal auditory brainstem response / MGI
- abnormal vestibular system physiology / MGI
- increased susceptibility to age-related hearing loss / MGI
- absent linear vestibular evoked potential / MGI
- head tilt / MGI
- head tossing / MGI
- novel environmental response-related retropulsion / MGI
- homeostasis/metabolism phenotype / MGI
- hearing/vestibular/ear phenotype / MGI
- behavior/neurological phenotype / MGI
- vision/eye phenotype / MGI
- abnormal eye electrophysiology / MGI
- impaired hearing / MGI
- absent pinna reflex / MGI
- absent startle reflex / MGI
- abnormal inner hair cell kinocilium morphology / MGI
- abnormal outer hair cell kinocilium morphology / MGI
- increased or absent threshold for auditory brainstem response / MGI
- decreased a wave implicit time / MGI
- decreased b wave implicit time / MGI