B6(Cg)-Gata1tm(avidin) Tg(Hnrnpa2b1/Cbx1-birA)1Jstr/Flmg

Status

Available to order

EMMA IDEM:15433
International strain nameB6(Cg)-Gata1tm(avidin) Tg(Hnrnpa2b1/Cbx1-birA)1Jstr/Flmg
Alternative nameAviGata1short/BirA
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolGata1tm(avidin), Tg(Hnrnpa2b1/Cbx1-birA)1Jstr
Gene/Transgene symbolGata1, Tg(Hnrnpa2b1/Cbx1-birA)1Jstr

Information from provider

ProviderJohn Strouboulis
Provider affiliationSchool of Cancer and Pharmaceutical Sciences, King's College London
Additional ownerProfessor Paresh Vyas, Oxford University, Radcliffe Department of Medicine, John Radcliffe Hospital, Headington, Oxford, United Kingdom
Genetic informationMice carry a targeted X-linked Gata1 gene mutation such that the second exon is removed and replaced by an in-frame fusion of the short (14 aa) biotinylatable avidin tag. In this way, the mice express an avidin-tagged, N-terminally truncated mutant of GATA1, called GATA1short. The mouse strain is also transgenic for the BirA bacterial biotin ligase under the control of the hnRNPA2 promoter (see description under EMMA strain ID EM:09008). As a result, this mouse strain expresses in vivo biotinylated GATA1short protein in tissues where the Gata1 gene is normally active (mainly erythroid cells and megakaryocytes).
Phenotypic informationHomozygous:
Embryos homozygous for the targeted GATA1short deletion present with a transient expansion of megakaryocytes with impaired erythropoiesis around E11.5-E14.5. Impaired erythropoiesis results in transient anemia which is lethal in utero for 10-15% of the embryos (mostly male; Gata1 is an X-linked gene). In adult mice, bone marrow erythropoiesis is perturbed with delayed maturation of erythroid cells. A slightly enlarged spleen with erythropoietic activity. Overall, a mild phenotype. No phenotype observed in mice transgenic for the 3xHA BirA/hnRNPA2-CBX transgene.

Heterozygous:
None. For male mice, see above.
Breeding historyThis mouse strain has been continuously bred for the last 8 years or so.
References
  • Oncogenic Gata1 causes stage-specific megakaryocyte differentiation delay.;Juban Gaëtan, Sakakini Nathalie, Chagraoui Hedia, Cruz Hernandez David, Cheng Qian, Soady Kelly, Stoilova Bilyana, Garnett Catherine, Waithe Dominic, Otto Georg, Doondeea Jessica, Usukhbayar Batchimeg, Karkoulia Elena, Alexiou Maria, Strouboulis John, Morrissey Edward, Roberts Irene, Porcher Catherine, Vyas Paresh, ;2021;Haematologica;106;1106-1119; 32527952
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreB.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archivingwild-type C57BL/6J
Stage of embryosMorula

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (gene matching)
  • decreased hematocrit / MGI
  • abnormal leukocyte cell number / MGI
  • increased neutrophil cell number / MGI
  • abnormal thrombopoiesis / MGI
  • abnormal megakaryocyte differentiation / MGI
  • extramedullary hematopoiesis / MGI
  • abnormal erythropoiesis / MGI
  • decreased bone marrow cell number / MGI
  • abnormal liver morphology / MGI
  • liver hypoplasia / MGI
  • pale liver / MGI
  • abnormal spleen morphology / MGI
  • enlarged spleen / MGI
  • abnormal hematopoietic system physiology / MGI
  • anemia / MGI
  • abnormal myelopoiesis / MGI
  • pale yolk sac / MGI
  • autoimmune response / MGI
  • premature death / MGI
  • abnormal definitive hematopoiesis / MGI
  • abnormal respiratory system physiology / MGI
  • no abnormal phenotype detected / MGI
  • abnormal bronchiole morphology / MGI
  • abnormal spleen red pulp morphology / MGI
  • abnormal bone marrow morphology / MGI
  • abnormal megakaryocyte progenitor cell morphology / MGI
  • abnormal proerythroblast morphology / MGI
  • abnormal megakaryocyte morphology / MGI
  • abnormal hematocrit / MGI
  • poikilocytosis / MGI
  • decreased erythrocyte cell number / MGI
  • increased erythroid progenitor cell number / MGI
  • thrombocytopenia / MGI
  • abnormal platelet shape / MGI
  • pallor / MGI
  • polyploidy / MGI
  • increased spleen weight / MGI
  • increased osteoclast cell number / MGI
  • increased osteoblast cell number / MGI
  • decreased eosinophil cell number / MGI
  • decreased lymphocyte cell number / MGI
  • polychromatophilia / MGI
  • respiratory system phenotype / MGI
  • hematopoietic system phenotype / MGI
  • abnormal skin physiology / MGI
  • abnormal common myeloid progenitor cell morphology / MGI
  • increased megakaryocyte cell number / MGI
  • decreased spleen red pulp amount / MGI
  • decreased spleen white pulp amount / MGI
  • decreased circulating interleukin-4 level / MGI
  • decreased circulating interleukin-13 level / MGI
  • decreased survivor rate / MGI
  • abnormal erythroid progenitor cell morphology / MGI
  • abnormal physiological response to xenobiotic / MGI
  • decreased erythroid progenitor cell number / MGI
  • pale spleen / MGI
  • increased splenocyte number / MGI
  • abnormal hemostasis / MGI
  • increased number of Howell-Jolly bodies / MGI
  • increased bone trabecula number / MGI
  • increased trabecular bone volume / MGI
  • increased compact bone volume / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • prenatal lethality, incomplete penetrance / MGI
  • embryonic lethality during organogenesis, incomplete penetrance / MGI
  • lethality throughout fetal growth and development, incomplete penetrance / MGI
  • myelofibrosis / MGI
  • increased bone ossification / MGI

Literature references

  • Oncogenic Gata1 causes stage-specific megakaryocyte differentiation delay.;Juban Gaëtan, Sakakini Nathalie, Chagraoui Hedia, Cruz Hernandez David, Cheng Qian, Soady Kelly, Stoilova Bilyana, Garnett Catherine, Waithe Dominic, Otto Georg, Doondeea Jessica, Usukhbayar Batchimeg, Karkoulia Elena, Alexiou Maria, Strouboulis John, Morrissey Edward, Roberts Irene, Porcher Catherine, Vyas Paresh, ;2021;Haematologica;106;1106-1119; 32527952

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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