IMPC phenotypes (gene matching)
- decreased body weight / IMPC
B6J.Cg-Atp5if1tm1b(EUCOMM)Wtsi/Cnbc
| Status | Available to order |
| EMMA ID | EM:15298 |
| International strain name | B6J.Cg-Atp5if1tm1b(EUCOMM)Wtsi/Cnbc |
| Alternative name | Atp5if1-global KO also named Global IF1 knockout |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Atp5if1tm1b(EUCOMM)Wtsi |
| Gene/Transgene symbol | Atp5if1 |
Information from provider
| Provider | José M Cuezva |
| Provider affiliation | Centro de Biología Molecular, Universidad Autónoma de Madrid |
| Genetic information | We used the Atp5if1-floxed mouse line generated and described in our PLoS Biol 2021 publication (doi.org/10.1371/journal.pbio.3001252) and the B6.Cg-Tg(Camk2a-cre)T29-1Stl/J mouse strain. The latter expresses cre recombinase in neurons and in the germline. Germline recombination of the floxed Atp5if1 (IF1) allele was assessed by PCR in different tissues and its inheritance by breeding with wild-type C57BL/6J mice verified in tissues that naturally express IF1, which showed no expression of the protein (see Commun Biol 2023, 6:836, doi.org/10.1038/s42003-023-05214-1). |
| Phenotypic information | Homozygous:None except when stressed.Heterozygous:Not studied. |
| Breeding history | 6-7 generations in C57BL/6J. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6J |
Disease and phenotype information
Literature references
- IF1 promotes oligomeric assemblies of sluggish ATP synthase and outlines the heterogeneity of the mitochondrial membrane potential.;Romero-Carramiñana Inés, Esparza-Moltó Pau B, Domínguez-Zorita Sonia, Nuevo-Tapioles Cristina, Cuezva José M, ;2023;Communications biology;6;836; 37573449