MGI phenotypes (allele matching)
- decreased rhombomere 3 size / MGI
STOCK Egr2tm3Pch Egr3tm1Jmi Tg(CD2-icre)4Kio Tg(Tbx21-AmCyan)Jfz/H
| Status | Only small colony available |
| EMMA ID | EM:15287 |
| Citation information | RRID:IMSR_EM:15287 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | STOCK Egr2tm3Pch Egr3tm1Jmi Tg(CD2-icre)4Kio Tg(Tbx21-AmCyan)Jfz/H |
| Alternative name | AmCyanTbet-Egr3/2 double Knockout |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(CD2-icre)4Kio, Egr2tm3Pch, Egr3tm1Jmi, Tg-AmCyanTbet |
| Gene/Transgene symbol | Tg(CD2-icre)4Kio, Egr2, Egr3, Tg-AmCyanTbet |
Information from provider
| Provider | Suling Li |
| Provider affiliation | Brunel University |
| Genetic information | AmCyan-T-bet reporter mouse line (Yu et al., Nat. Immunol., 2015) crossed with a Egr3-/- and CD2-specific Egr2-/-(CD2-Egr2-/-;Egr3-/-) mice line, which was generated by interbreeding Egr3 KO (Tourtellotte and Milbrandt, Nat Genet 1998; MGI:2180063, symbol Egr3tm1Jmi) and CD2-specific Egr2 KO mice (Zhu et al., J Exp Med 2008; Li et al., Immunity 2012; Egr2-flox: MGI:2183227, symbol Egr2tm3Pch; CD2-Cre: MGI:2449947, symbol Tg(CD2-icre)4Kio). More than 30 backcrosses. |
| Phenotypic information | Homozygous:The knock-out mice with deletion of both transcription factors Egr2 and Egr3 in lymphocytes resulted in a lethal autoimmune syndrome with excessive serum proinflammatory cytokines but also impaired antigen receptor-induced proliferation of B and T cells.Heterozygous:Inflammation similar as homozygous but less severe. |
| Breeding history | The AmCyan-T-bet reporter mouse line (Yu et al., 2015) was crossed with a Egr3-/- and CD2-specific Egr2-/- (CD2-Egr2-/-Egr3-/-) mice line, which was generated by interbreeding Egr3 knock-out (Tourtellotte and Milbrandt, 1998) and CD2-specific Egr2 knock-out mice (Zhu et al., J Exp Med 2008; Li et al., Immunity 2012). More than 30 backcrosses. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Dejerine-Sottas syndrome / Orphanet_64748
- Charcot-Marie-Tooth disease type 1D / Orphanet_101084
- Charcot-Marie-Tooth disease type 4E / Orphanet_99951
MGI phenotypes (gene matching)
- abnormal mandible morphology / MGI
- tremors / MGI
- weakness / MGI
- paralysis / MGI
- hindlimb paralysis / MGI
- abnormal myelination / MGI
- abnormal rhombomere morphology / MGI
- abnormal motor neuron morphology / MGI
- abnormal spinal cord morphology / MGI
- abnormal neuromuscular synapse morphology / MGI
- abnormal cranial nerve morphology / MGI
- abnormal trigeminal nerve morphology / MGI
- abnormal abducens nerve morphology / MGI
- abnormal facial nerve morphology / MGI
- abnormal glossopharyngeal nerve morphology / MGI
- abnormal vagus nerve morphology / MGI
- abnormal cranial ganglia morphology / MGI
- abnormal geniculate ganglion morphology / MGI
- abnormal trigeminal ganglion morphology / MGI
- abnormal glossopharyngeal ganglion morphology / MGI
- abnormal vagus ganglion morphology / MGI
- abnormal Schwann cell morphology / MGI
- decreased body weight / MGI
- weight loss / MGI
- decreased body size / MGI
- delayed eyelid opening / MGI
- hypoactivity / MGI
- impaired coordination / MGI
- abnormal gait / MGI
- impaired limb coordination / MGI
- postnatal growth retardation / MGI
- respiratory distress / MGI
- abnormal motor capabilities/coordination/movement / MGI
- abnormal skeleton development / MGI
- abnormal neural tube morphology / MGI
- abnormal sciatic nerve morphology / MGI
- abnormal aortic valve morphology / MGI
- abnormal somatic nervous system morphology / MGI
- short tibia / MGI
- abnormal cochlear ganglion morphology / MGI
- abnormal bone mineralization / MGI
- no phenotypic analysis / MGI
- abnormal long bone epiphyseal plate morphology / MGI
- short femur / MGI
- decreased width of hypertrophic chondrocyte zone / MGI
- abnormal long bone morphology / MGI
- abnormal bone structure / MGI
- short radius / MGI
- fusion of glossopharyngeal and vagus nerve / MGI
- decreased length of long bones / MGI
- increased pulmonary respiratory rate / MGI
- abnormal neuronal migration / MGI
- abnormal hindbrain development / MGI
- decreased diameter of long bones / MGI
- decreased diameter of femur / MGI
- decreased diameter of radius / MGI
- decreased diameter of tibia / MGI
- decreased nerve conduction velocity / MGI
- enlarged aortic valve / MGI
- thick aortic valve cusps / MGI
- thick pulmonary valve cusps / MGI
- integument phenotype / MGI
- lethality at weaning, complete penetrance / MGI
- postnatal lethality, complete penetrance / MGI
- postnatal lethality, incomplete penetrance / MGI
- neonatal lethality, incomplete penetrance / MGI
- increased Schwann cell proliferation / MGI
- decreased rhombomere 3 size / MGI
- absent rhombomere 3 / MGI
- decreased rhombomere 5 size / MGI
- absent rhombomere 5 / MGI
- decreased rhombomere 6 size / MGI