STOCK Egr2tm3Pch Egr3tm1Jmi Tg(CD2-icre)4Kio Tg(Tbx21-AmCyan)Jfz/H

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EMMA IDEM:15287
International strain nameSTOCK Egr2tm3Pch Egr3tm1Jmi Tg(CD2-icre)4Kio Tg(Tbx21-AmCyan)Jfz/H
Alternative nameAmCyanTbet-Egr3/2 double Knockout
Strain typeTransgenic Strains
Allele/Transgene symbolTg-AmCyanTbet
Gene/Transgene symbolTg-AmCyanTbet

Information from provider

ProviderSuling Li
Provider affiliationBrunel University
Genetic informationAmCyan-T-bet reporter mouse line (Yu et al., Nat. Immunol., 2015) crossed with a Egr3-/- and CD2-specific Egr2-/-(CD2-Egr2-/-;Egr3-/-) mice line, which was generated by interbreeding Egr3 KO (Tourtellotte and Milbrandt, Nat Genet 1998; MGI:2180063, symbol Egr3tm1Jmi) and CD2-specific Egr2 KO mice (Zhu et al., J Exp Med 2008; Li et al., Immunity 2012; Egr2-flox: MGI:2183227, symbol Egr2tm3Pch; CD2-Cre: MGI:2449947, symbol Tg(CD2-icre)4Kio). More than 30 backcrosses.
Phenotypic informationHomozygous:
The knock-out mice with deletion of both transcription factors Egr2 and Egr3 in lymphocytes resulted in a lethal autoimmune syndrome with excessive serum proinflammatory cytokines but also impaired antigen receptor-induced proliferation of B and T cells.

Heterozygous:
Inflammation similar as homozygous but less severe.
Breeding historyThe AmCyan-T-bet reporter mouse line (Yu et al., 2015) was crossed with a Egr3-/- and CD2-specific Egr2-/- (CD2-Egr2-/-Egr3-/-) mice line, which was generated by interbreeding Egr3 knock-out (Tourtellotte and Milbrandt, 1998) and CD2-specific Egr2 knock-out mice (Zhu et al., J Exp Med 2008; Li et al., Immunity 2012). More than 30 backcrosses.
ReferencesNone available
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • decreased rhombomere 3 size / MGI

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