B6;CBA-Tg(H4C16-mCherry)/Cnbc
| Status | Available to order |
| EMMA ID | EM:15239 |
| International strain name | B6;CBA-Tg(H4C16-mCherry)/Cnbc |
| Alternative name | B6.H4-mCherry |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(H4C16-mCherry) |
| Gene/Transgene symbol | Tg(H4C16-mCherry) |
Information from provider
| Provider | Lluís Espinosa |
| Provider affiliation | Cancer, IMIM (Institut de Recerca Hospital del Mar) |
| Additional owner | PBRPB CAG-mCherry-IRES-PURO plasmid from Addgene (#106333) was used for the generation of this mutant. |
| Genetic information | A Homo sapiens H4 histone 16 (H4C16) mRNA (NCBI Reference Sequence: NM_175054.2) was fused to an mCherry protein-coding sequence. This construct was cloned into piggy bac transposon system. The line was generated by pronuclear injection of B6CBA zygotes with 30 ng/μl hyPBase mRNA+ 30 ng/μl PB_CAG_Cherry-histoneH4 plasmid (BRPB CAG-mCherry-IRES-PURO Addgene #106333) and transfer into pseudo-pregnant 0.5 dpc CD1 females. |
| Phenotypic information | Homozygous:Unknown.Heterozygous:No phenotypic effects in heterozygous mice. |
| Breeding history | A male H4-mCherry from the first generation was crossed with C57BL/6 females and transgene positive male offspring were subsequently mated to C57BL/6 females. This mating scheme was repeated for 17 generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
Literature references
- Dynamic chromatin association of IκBα is regulated by acetylation and cleavage of histone H4.;Marruecos Laura, Bertran Joan, Álvarez-Villanueva Daniel, Mulero María Carmen, Guillén Yolanda, Palma Luis G, Floor Martin, Vert Anna, Arce-Gallego Sara, Pecharroman Irene, Batlle Laura, Villà-Freixa Jordi, Ghosh Gourisankar, Bigas Anna, Espinosa Lluís, ;2021;EMBO reports;22;e52649; 34224210