B6Brd;B6N-Tyrc-Brd Snx5tm1a(KOMP)Wtsi/WtsiPh

Status

Under development - register interest

EMMA IDEM:15202
International strain nameB6Brd;B6N-Tyrc-Brd Snx5tm1a(KOMP)Wtsi/WtsiPh
Alternative nameEPD0033_3_D07
Strain typeTargeted Mutant Strains
Allele/Transgene symbolSnx5tm1a(KOMP)Wtsi
Gene/Transgene symbolSnx5
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from KOMP ES clone EPD0033_3_D07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centreInstitute of Molecular Genetics, Prague, Czech Republic

Disease and phenotype information

IMPC phenotypes (allele matching)
  • decreased circulating glucose level / IMPC
  • decreased circulating potassium level / IMPC
IMPC phenotypes (gene matching)
  • decreased circulating glucose level / IMPC
  • abnormal vertebrae morphology / IMPC
  • increased hemoglobin content / IMPC
  • decreased hematocrit / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • decreased circulating iron level / IMPC
  • decreased blood urea nitrogen level / IMPC
  • decreased mean corpuscular volume / IMPC
  • decreased circulating potassium level / IMPC
MGI phenotypes (allele matching)
  • decreased circulating LDL cholesterol level / MGI
  • decreased circulating HDL cholesterol level / MGI
  • increased body weight / MGI
  • increased hematocrit / MGI
  • decreased circulating alanine transaminase level / MGI
  • decreased circulating cholesterol level / MGI
  • decreased circulating potassium level / MGI
MGI phenotypes (gene matching)
  • decreased circulating LDL cholesterol level / MGI
  • decreased circulating HDL cholesterol level / MGI
  • increased body weight / MGI
  • decreased body weight / MGI
  • decreased body size / MGI
  • cyanosis / MGI
  • postnatal growth retardation / MGI
  • respiratory failure / MGI
  • abnormal coat/hair pigmentation / MGI
  • abnormal pulmonary alveolus morphology / MGI
  • increased hematocrit / MGI
  • decreased circulating alanine transaminase level / MGI
  • decreased circulating cholesterol level / MGI
  • decreased circulating potassium level / MGI
  • decreased lung weight / MGI
  • decreased type I pneumocyte number / MGI
  • primary atelectasis / MGI
  • abnormal pulmonary alveolar sac morphology / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • neonatal lethality, incomplete penetrance / MGI
  • perinatal lethality, incomplete penetrance / MGI

Literature references

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information on how we integrate external resources can be found here

Register interest

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

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    Practical information

    Example health report
    (Current health report will be provided later)

    Material Transfer Agreement (MTA)
    Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

    EMMA conditions
    Legally binding conditions for the transfer

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